BGL04_ARATH
ID BGL04_ARATH Reviewed; 512 AA.
AC Q9ZUI3;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Beta-glucosidase 4;
DE Short=AtBGLU4;
DE EC=3.2.1.21;
DE Flags: Precursor;
GN Name=BGLU4; OrderedLocusNames=At1g60090; ORFNames=T2K10.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15604686; DOI=10.1007/s11103-004-0790-1;
RA Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R.,
RA Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.;
RT "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase
RT family 1.";
RL Plant Mol. Biol. 55:343-367(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC with release of beta-D-glucose.; EC=3.2.1.21;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD14488.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005966; AAD14488.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33658.1; -; Genomic_DNA.
DR PIR; E96625; E96625.
DR RefSeq; NP_176217.2; NM_104701.3.
DR AlphaFoldDB; Q9ZUI3; -.
DR SMR; Q9ZUI3; -.
DR STRING; 3702.AT1G60090.1; -.
DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR PaxDb; Q9ZUI3; -.
DR PRIDE; Q9ZUI3; -.
DR ProteomicsDB; 240861; -.
DR EnsemblPlants; AT1G60090.1; AT1G60090.1; AT1G60090.
DR GeneID; 842304; -.
DR Gramene; AT1G60090.1; AT1G60090.1; AT1G60090.
DR KEGG; ath:AT1G60090; -.
DR Araport; AT1G60090; -.
DR TAIR; locus:2202710; AT1G60090.
DR eggNOG; KOG0626; Eukaryota.
DR HOGENOM; CLU_001859_1_0_1; -.
DR InParanoid; Q9ZUI3; -.
DR OrthoDB; 408001at2759; -.
DR PhylomeDB; Q9ZUI3; -.
DR BioCyc; ARA:AT1G60090-MON; -.
DR PRO; PR:Q9ZUI3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9ZUI3; baseline and differential.
DR Genevisible; Q9ZUI3; AT.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; PTHR10353; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..512
FT /note="Beta-glucosidase 4"
FT /id="PRO_0000389566"
FT ACT_SITE 186
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 391
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 43
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 140
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 185
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 328
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 446
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 453..454
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 211
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 216
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 436
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 468
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 205..212
FT /evidence="ECO:0000250"
SQ SEQUENCE 512 AA; 57971 MW; 429AC4ACBA52FC98 CRC64;
MEQILALFAI FLAFAFSGKC SDVFSRSDYP EGFVFGAGTS AYQWEGAAAE DGRKPSLWDT
LCHSRDQGNG DIACDGYHKY KDDVKLMVDT NLDAFRFSIS WSRLIPNGRG PVNQKGLQFY
KNLIQELVSH GIEPHVTLYH YDHPQSLEDE YGGWLNHRMI KDFTTYADVC FREFGNHVKL
WTTINEANIF SIGGYNDGDT PPGRCSKPSK NCSSGNSSIE PYIVGHNLLL AHASVSRRYK
QKYKDKQGGS IGFSLFILGL IPTTSSKDDA TATQRAQDFY VGWFLRPLLF GDYPDTMKRT
IGSRLPVFSE KESEQVKGSC DFVGVIHYHA ASVTNIKSKP SLSGNPDFYS YMETDFGKSL
DFQYANTPWA MEVVLEYIKQ SYGNPPVYIL ESAKFTFQWQ QIGTPMKQDS QLKQKDIPRV
EYLHAYIGGV LKSIRNGSDT RGYFVWSFMD LYELLGGYEV GFGLYTVNFS DPHRKRSPKL
SAYWYSDFLK GESAFLDSQG IKELQSKYSS SF
