BGL05_ORYSJ
ID BGL05_ORYSJ Reviewed; 513 AA.
AC Q5JK35; A0A0P0VCE1; Q0JGC3;
DT 15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Beta-glucosidase 5;
DE Short=Os1bglu5;
DE EC=3.2.1.21;
DE Flags: Precursor;
GN Name=BGLU5; OrderedLocusNames=Os01g0930800, LOC_Os01g70520;
GN ORFNames=OSJNBa0052O12.35, P0506E04.13;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17196101; DOI=10.1186/1471-2229-6-33;
RA Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A.,
RA Ketudat Cairns J.R.;
RT "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12
RT beta-glucosidase.";
RL BMC Plant Biol. 6:33-33(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC with release of beta-D-glucose.; EC=3.2.1.21;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF07205.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AP003272; BAD87322.1; -; Genomic_DNA.
DR EMBL; AP004330; BAD88178.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF07205.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014957; BAS76034.1; -; Genomic_DNA.
DR EMBL; AK119221; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015615756.1; XM_015760270.1.
DR AlphaFoldDB; Q5JK35; -.
DR SMR; Q5JK35; -.
DR STRING; 4530.OS01T0930800-01; -.
DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR PaxDb; Q5JK35; -.
DR PRIDE; Q5JK35; -.
DR EnsemblPlants; Os01t0930800-01; Os01t0930800-01; Os01g0930800.
DR GeneID; 4326948; -.
DR Gramene; Os01t0930800-01; Os01t0930800-01; Os01g0930800.
DR KEGG; osa:4326948; -.
DR eggNOG; KOG0626; Eukaryota.
DR HOGENOM; CLU_001859_1_0_1; -.
DR InParanoid; Q5JK35; -.
DR OMA; MSRAFRC; -.
DR OrthoDB; 408001at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q5JK35; OS.
DR GO; GO:0033907; F:beta-D-fucosidase activity; IEA:UniProt.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProt.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; PTHR10353; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..513
FT /note="Beta-glucosidase 5"
FT /id="PRO_0000390324"
FT ACT_SITE 192
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 405
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 46
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 336
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 447
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 454..455
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 224
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 273
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 412
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 211..220
FT /evidence="ECO:0000250"
FT CONFLICT 19
FT /note="G -> C (in Ref. 5; AK119221)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="F -> L (in Ref. 5; AK119221)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 513 AA; 57448 MW; 4E72B6E3037E507C CRC64;
MAAAIAVVYL SLLLLLLHGA APAVLGYTRG DFPEDFVFGS ATSSYQYEGG FDEDGRSPSN
WDIFTHQGKM PGRSTADVAA DGYHKYKDDL KLMVDTNLEA YRLSISWSRI IPNGRGDVNP
KGLQYYNDII DGLVKNGIQV HIMLYQLDLP QVLEDEYDGW LSPRILEDFK AYADVCFKEF
GDRVAHWITI DEPNVASIGS YDSGQLAPGR CSDPFGIRKC TVGNSSVEPY IAVHNMLLAH
ASVTKLYREK YQVAGKGIIG ISVYTFWAYP LTNSTVDLEA TKRCQDFIVH WVLRPLVFGD
YPQVMKNIVG SRLPSFTKAQ SEDVKGSLDF IGMNHYYSLY VNDRPLGKGT RDFVADISIY
YRGSKTDPPP GKAAPTSIGP DPQGLRLMVQ YLQETYGNLP IYILENGYGS SNDTVHDNDR
VDYLKSYIGS ILTALRNGAN VKGYFVWSFV DVFEYLTGYG QSYGLYRVDF ADESRPRQAR
LSARWYSGFL KNREMDVDQS ELAMAAAESR AQQ
