SEPS_METBU
ID SEPS_METBU Reviewed; 539 AA.
AC Q12XG0;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=O-phosphoserine--tRNA(Cys) ligase {ECO:0000255|HAMAP-Rule:MF_01674};
DE Short=O-phosphoserine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_01674};
DE EC=6.1.1.27 {ECO:0000255|HAMAP-Rule:MF_01674};
DE AltName: Full=Non-canonical O-phosphoseryl-tRNA(Cys) synthetase {ECO:0000255|HAMAP-Rule:MF_01674};
DE AltName: Full=O-phosphoseryl-tRNA(Cys) synthetase {ECO:0000255|HAMAP-Rule:MF_01674};
DE Short=SepRS {ECO:0000255|HAMAP-Rule:MF_01674};
GN Name=sepS {ECO:0000255|HAMAP-Rule:MF_01674}; OrderedLocusNames=Mbur_0923;
OS Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 /
OS ACE-M).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanococcoides.
OX NCBI_TaxID=259564;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6242 / NBRC 107633 / OCM 468 / ACE-M;
RX PubMed=19404327; DOI=10.1038/ismej.2009.45;
RA Allen M.A., Lauro F.M., Williams T.J., Burg D., Siddiqui K.S.,
RA De Francisci D., Chong K.W., Pilak O., Chew H.H., De Maere M.Z., Ting L.,
RA Katrib M., Ng C., Sowers K.R., Galperin M.Y., Anderson I.J., Ivanova N.,
RA Dalin E., Martinez M., Lapidus A., Hauser L., Land M., Thomas T.,
RA Cavicchioli R.;
RT "The genome sequence of the psychrophilic archaeon, Methanococcoides
RT burtonii: the role of genome evolution in cold adaptation.";
RL ISME J. 3:1012-1035(2009).
CC -!- FUNCTION: Catalyzes the attachment of O-phosphoserine (Sep) to
CC tRNA(Cys). {ECO:0000255|HAMAP-Rule:MF_01674}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + O-phospho-L-serine + tRNA(Cys) = AMP + diphosphate + O-
CC phospho-L-seryl-tRNA(Cys); Xref=Rhea:RHEA:25678, Rhea:RHEA-COMP:9661,
CC Rhea:RHEA-COMP:9719, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57524, ChEBI:CHEBI:78442, ChEBI:CHEBI:78551,
CC ChEBI:CHEBI:456215; EC=6.1.1.27; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01674};
CC -!- SUBUNIT: Homotetramer. Interacts with SepCysS. {ECO:0000255|HAMAP-
CC Rule:MF_01674}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC O-phosphoseryl-tRNA(Cys) synthetase subfamily. {ECO:0000255|HAMAP-
CC Rule:MF_01674}.
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DR EMBL; CP000300; ABE51866.1; -; Genomic_DNA.
DR RefSeq; WP_011499016.1; NC_007955.1.
DR AlphaFoldDB; Q12XG0; -.
DR SMR; Q12XG0; -.
DR STRING; 259564.Mbur_0923; -.
DR EnsemblBacteria; ABE51866; ABE51866; Mbur_0923.
DR GeneID; 3998666; -.
DR KEGG; mbu:Mbur_0923; -.
DR HOGENOM; CLU_506822_0_0_2; -.
DR OMA; RSHMTSG; -.
DR OrthoDB; 6499at2157; -.
DR Proteomes; UP000001979; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0043816; F:phosphoserine-tRNA(Cys) ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR GO; GO:0043039; P:tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.930.10; -; 1.
DR HAMAP; MF_01674; Sep_tRNA_synth; 1.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR005246; O-Pseryl-tRNA(Cys)_ligase.
DR InterPro; IPR002319; Phenylalanyl-tRNA_Synthase.
DR InterPro; IPR041590; SepRS_C.
DR PANTHER; PTHR11538:SF38; PTHR11538:SF38; 1.
DR Pfam; PF18006; SepRS_C; 1.
DR Pfam; PF01409; tRNA-synt_2d; 1.
DR SUPFAM; SSF55681; SSF55681; 1.
DR TIGRFAMs; TIGR00470; sepS; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..539
FT /note="O-phosphoserine--tRNA(Cys) ligase"
FT /id="PRO_0000363748"
FT BINDING 188..190
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01674"
FT BINDING 233..235
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01674"
FT BINDING 275..276
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01674"
FT BINDING 327
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01674"
SQ SEQUENCE 539 AA; 61176 MW; 69075BEAC71BD47B CRC64;
MKFDPESIKK AAKEDFDSAW TSGKDLIKKT GLNQQYPHTS FHFGKAHPVY DTIAKLREAY
LRMGFDEMMN PLIVDEKEVY KQFGHEALAV LDRCYYLAGL PRPNVGISDQ RIAKIKEMLG
GIDDEGIETI RKVLHSYKKG EVEGDDLVPE IALKLNVSDA LVVEMIDKVF PEFKELTPQA
TTKTLRSHMT SGWFISLSGI LERSRPPFHL FSIDRSFRRE QQEDASRLMT YYSASCVIMD
EDVTVDHGKA VAQGLLAQFG FEKFMFRPDE KRSKYYVPDT QIEVFAYHPK LVGSNTKYSD
GWIEIATFGI YSPTALAEYN IPCPVMNLGL GVERLAMILH DSTDLRGMTY PQLPQYAEWE
LKDNELARMI FVDKLPETPE GQEILEGIVM QCDMHGSEPS PCEFVAWEGI LKGKKVKVSV
IEPEEDTKLC GPAAYNEVLV HENDVLGLPN NKKWKKAFEN HSARTGVRFI EAFAAQAAKE
IEEAVEKGEK ECETRVRIVK VPSEINIKLD PLAQRYITGK KQKIDIRGPV FTTVRAEIE
