SEPS_METMP
ID SEPS_METMP Reviewed; 537 AA.
AC Q6LZE1;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=O-phosphoserine--tRNA(Cys) ligase {ECO:0000255|HAMAP-Rule:MF_01674};
DE Short=O-phosphoserine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_01674};
DE EC=6.1.1.27 {ECO:0000255|HAMAP-Rule:MF_01674};
DE AltName: Full=Non-canonical O-phosphoseryl-tRNA(Cys) synthetase {ECO:0000255|HAMAP-Rule:MF_01674};
DE AltName: Full=O-phosphoseryl-tRNA(Cys) synthetase {ECO:0000255|HAMAP-Rule:MF_01674};
DE Short=SepRS {ECO:0000255|HAMAP-Rule:MF_01674};
GN Name=sepS {ECO:0000255|HAMAP-Rule:MF_01674}; OrderedLocusNames=MMP0688;
OS Methanococcus maripaludis (strain S2 / LL).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanococcaceae; Methanococcus.
OX NCBI_TaxID=267377;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S2 / LL;
RX PubMed=15466049; DOI=10.1128/jb.186.20.6956-6969.2004;
RA Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J.,
RA Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M.,
RA Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A.,
RA Moore B.C., Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D.,
RA Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W.,
RA Olson M.V., Leigh J.A.;
RT "Complete genome sequence of the genetically tractable hydrogenotrophic
RT methanogen Methanococcus maripaludis.";
RL J. Bacteriol. 186:6956-6969(2004).
CC -!- FUNCTION: Catalyzes the attachment of O-phosphoserine (Sep) to
CC tRNA(Cys). {ECO:0000255|HAMAP-Rule:MF_01674}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + O-phospho-L-serine + tRNA(Cys) = AMP + diphosphate + O-
CC phospho-L-seryl-tRNA(Cys); Xref=Rhea:RHEA:25678, Rhea:RHEA-COMP:9661,
CC Rhea:RHEA-COMP:9719, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57524, ChEBI:CHEBI:78442, ChEBI:CHEBI:78551,
CC ChEBI:CHEBI:456215; EC=6.1.1.27; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01674};
CC -!- SUBUNIT: Homotetramer. Interacts with SepCysS. {ECO:0000255|HAMAP-
CC Rule:MF_01674}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC O-phosphoseryl-tRNA(Cys) synthetase subfamily. {ECO:0000255|HAMAP-
CC Rule:MF_01674}.
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DR EMBL; BX950229; CAF30244.1; -; Genomic_DNA.
DR RefSeq; WP_011170632.1; NC_005791.1.
DR PDB; 2ODR; X-ray; 3.23 A; A/B/C/D=1-537.
DR PDBsum; 2ODR; -.
DR AlphaFoldDB; Q6LZE1; -.
DR SMR; Q6LZE1; -.
DR DIP; DIP-60876N; -.
DR STRING; 267377.MMP0688; -.
DR EnsemblBacteria; CAF30244; CAF30244; MMP0688.
DR GeneID; 2762711; -.
DR KEGG; mmp:MMP0688; -.
DR PATRIC; fig|267377.15.peg.705; -.
DR eggNOG; arCOG00411; Archaea.
DR HOGENOM; CLU_506822_0_0_2; -.
DR OMA; RSHMTSG; -.
DR OrthoDB; 6499at2157; -.
DR BioCyc; MMAR267377:MMP_RS03605-MON; -.
DR BRENDA; 6.1.1.27; 3262.
DR EvolutionaryTrace; Q6LZE1; -.
DR Proteomes; UP000000590; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0043816; F:phosphoserine-tRNA(Cys) ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR GO; GO:0043039; P:tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.930.10; -; 1.
DR HAMAP; MF_01674; Sep_tRNA_synth; 1.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR005246; O-Pseryl-tRNA(Cys)_ligase.
DR InterPro; IPR002319; Phenylalanyl-tRNA_Synthase.
DR InterPro; IPR041590; SepRS_C.
DR PANTHER; PTHR11538:SF38; PTHR11538:SF38; 1.
DR Pfam; PF18006; SepRS_C; 1.
DR Pfam; PF01409; tRNA-synt_2d; 1.
DR SUPFAM; SSF55681; SSF55681; 1.
DR TIGRFAMs; TIGR00470; sepS; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..537
FT /note="O-phosphoserine--tRNA(Cys) ligase"
FT /id="PRO_0000363750"
FT BINDING 186..188
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01674"
FT BINDING 231..233
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01674"
FT BINDING 273..274
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01674"
FT BINDING 317
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01674"
FT HELIX 5..13
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 15..22
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 23..25
FT /evidence="ECO:0007829|PDB:2ODR"
FT TURN 31..33
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 47..61
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 71..74
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 75..82
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 83..85
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 86..91
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 94..98
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 180..183
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 188..198
FT /evidence="ECO:0007829|PDB:2ODR"
FT TURN 199..201
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 206..215
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 227..237
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 243..255
FT /evidence="ECO:0007829|PDB:2ODR"
FT TURN 256..258
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 262..266
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 280..286
FT /evidence="ECO:0007829|PDB:2ODR"
FT TURN 287..290
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 291..301
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 303..308
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 315..321
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 322..329
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 335..339
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 341..343
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 350..354
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 357..360
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 366..381
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 427..431
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 450..459
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 460..466
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 467..481
FT /evidence="ECO:0007829|PDB:2ODR"
FT STRAND 505..507
FT /evidence="ECO:0007829|PDB:2ODR"
FT HELIX 509..517
FT /evidence="ECO:0007829|PDB:2ODR"
SQ SEQUENCE 537 AA; 61148 MW; 3AB053C02E6571AB CRC64;
MFKREEIIEM ANKDFEKAWI ETKDLIKAKK INESYPRIKP VFGKTHPVND TIENLRQAYL
RMGFEEYINP VIVDERDIYK QFGPEAMAVL DRCFYLAGLP RPDVGLSDEK ISQIEKLGIK
VSEHKESLQK ILHGYKKGTL DGDDLVLEIS NALEISSEMG LKILEDVFPE FKDLTAVSSK
LTLRSHMTSG WFLTVSDLMN KKPLPFKLFS IDRCFRREQK EDKSHLMTYH SASCAIAGEG
VDINDGKAIA EGLLSQFGFT NFKFIPDEKK SKYYTPETQT EVYAYHPKLK EWLEVATFGV
YSPVALSKYG IDVPVMNLGL GVERLAMISG NFADVREMVY PQFYEHKLND RNVASMVKLD
KVPVMDEIYD LTKELIESCV KNKDLKSPCE LAIEKTFSFG KTKKNVKINI FEKEEGKNLL
GPSILNEIYV YDGNVIGIPE SFDGVKEEFK DFLEKGKSEG VATGIRYIDA LCFKITSKLE
EAFVSNTTEF KVKVPIVRSL SDINLKIDDI ALKQIMSKNK VIDVRGPVFL NVEVKIE
