SEQA_ACTSZ
ID SEQA_ACTSZ Reviewed; 212 AA.
AC A6VMY4;
DT 16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=Negative modulator of initiation of replication {ECO:0000255|HAMAP-Rule:MF_00908};
GN Name=seqA {ECO:0000255|HAMAP-Rule:MF_00908}; OrderedLocusNames=Asuc_0963;
OS Actinobacillus succinogenes (strain ATCC 55618 / DSM 22257 / CCUG 43843 /
OS 130Z).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Actinobacillus.
OX NCBI_TaxID=339671;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 55618 / DSM 22257 / CCUG 43843 / 130Z;
RX PubMed=21118570; DOI=10.1186/1471-2164-11-680;
RA McKinlay J.B., Laivenieks M., Schindler B.D., McKinlay A.A.,
RA Siddaramappa S., Challacombe J.F., Lowry S.R., Clum A., Lapidus A.L.,
RA Burkhart K.B., Harkins V., Vieille C.;
RT "A genomic perspective on the potential of Actinobacillus succinogenes for
RT industrial succinate production.";
RL BMC Genomics 11:680-680(2010).
CC -!- FUNCTION: Negative regulator of replication initiation, which
CC contributes to regulation of DNA replication and ensures that
CC replication initiation occurs exactly once per chromosome per cell
CC cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC region, thus preventing assembly of replication proteins and re-
CC initiation at newly replicated origins. Repression is relieved when the
CC region becomes fully methylated. {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000255|HAMAP-
CC Rule:MF_00908}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000746; ABR74331.1; -; Genomic_DNA.
DR RefSeq; WP_012072708.1; NC_009655.1.
DR AlphaFoldDB; A6VMY4; -.
DR SMR; A6VMY4; -.
DR STRING; 339671.Asuc_0963; -.
DR EnsemblBacteria; ABR74331; ABR74331; Asuc_0963.
DR KEGG; asu:Asuc_0963; -.
DR eggNOG; COG3057; Bacteria.
DR HOGENOM; CLU_099733_0_0_6; -.
DR OMA; RTRTYFA; -.
DR OrthoDB; 1625515at2; -.
DR Proteomes; UP000001114; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.1220.10; -; 1.
DR Gene3D; 1.20.1380.10; -; 1.
DR HAMAP; MF_00908; SeqA; 1.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR010985; Ribbon_hlx_hlx.
DR InterPro; IPR005621; SeqA.
DR InterPro; IPR026577; SeqA_DNA-bd_C.
DR InterPro; IPR036835; SeqA_DNA-bd_C_sf.
DR InterPro; IPR033761; SeqA_N.
DR Pfam; PF03925; SeqA; 1.
DR Pfam; PF17206; SeqA_N; 1.
DR PIRSF; PIRSF019401; SeqA; 1.
DR SUPFAM; SSF47598; SSF47598; 1.
DR SUPFAM; SSF82808; SSF82808; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication inhibitor; DNA-binding; Reference proteome.
FT CHAIN 1..212
FT /note="Negative modulator of initiation of replication"
FT /id="PRO_0000413917"
FT REGION 113..114
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
FT REGION 144..148
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
SQ SEQUENCE 212 AA; 23654 MW; 98F5E1309D84FEB1 CRC64;
MKIIEVDEEL YQFIASHTQS IGESASDILR RLLNLPTSSV SSVALETVAV ESAEAANPET
PSAVKNDFVL EPVAEKSSLE KTVKKQPEQV ICHIVNKVRQ VIESEAFQHE TKAVVRFLAI
LTALYRTNPE GFSLAIESEQ VQGRTRVYFA RDEATLLAAG THTKPKQIPD TPYWVITNNN
SGRKMIMLEG VMHGMQLPDE LIEDIRGYFI VN
