SEQA_ALIF1
ID SEQA_ALIF1 Reviewed; 180 AA.
AC Q5E6N6;
DT 16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Negative modulator of initiation of replication {ECO:0000255|HAMAP-Rule:MF_00908};
GN Name=seqA {ECO:0000255|HAMAP-Rule:MF_00908}; OrderedLocusNames=VF_0815;
OS Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Aliivibrio.
OX NCBI_TaxID=312309;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700601 / ES114;
RX PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT pathogenic congeners.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC -!- FUNCTION: Negative regulator of replication initiation, which
CC contributes to regulation of DNA replication and ensures that
CC replication initiation occurs exactly once per chromosome per cell
CC cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC region, thus preventing assembly of replication proteins and re-
CC initiation at newly replicated origins. Repression is relieved when the
CC region becomes fully methylated. {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000255|HAMAP-
CC Rule:MF_00908}.
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DR EMBL; CP000020; AAW85310.1; -; Genomic_DNA.
DR RefSeq; WP_005418290.1; NC_006840.2.
DR RefSeq; YP_204198.1; NC_006840.2.
DR AlphaFoldDB; Q5E6N6; -.
DR SMR; Q5E6N6; -.
DR STRING; 312309.VF_0815; -.
DR EnsemblBacteria; AAW85310; AAW85310; VF_0815.
DR GeneID; 64240768; -.
DR KEGG; vfi:VF_0815; -.
DR PATRIC; fig|312309.11.peg.808; -.
DR eggNOG; COG3057; Bacteria.
DR HOGENOM; CLU_099733_0_0_6; -.
DR OMA; RTRTYFA; -.
DR OrthoDB; 1625515at2; -.
DR Proteomes; UP000000537; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.1220.10; -; 1.
DR Gene3D; 1.20.1380.10; -; 1.
DR HAMAP; MF_00908; SeqA; 1.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR010985; Ribbon_hlx_hlx.
DR InterPro; IPR005621; SeqA.
DR InterPro; IPR026577; SeqA_DNA-bd_C.
DR InterPro; IPR036835; SeqA_DNA-bd_C_sf.
DR InterPro; IPR033761; SeqA_N.
DR Pfam; PF03925; SeqA; 1.
DR Pfam; PF17206; SeqA_N; 1.
DR PIRSF; PIRSF019401; SeqA; 1.
DR SUPFAM; SSF47598; SSF47598; 1.
DR SUPFAM; SSF82808; SSF82808; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication inhibitor; DNA-binding; Reference proteome.
FT CHAIN 1..180
FT /note="Negative modulator of initiation of replication"
FT /id="PRO_0000413944"
FT REGION 115..119
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
SQ SEQUENCE 180 AA; 20208 MW; 6512634C1BFA578B CRC64;
MKTIEVDEEL YRFIASQTQH IGESASDILR RLLMQSSPSD LAVSAPVEVQ KKGIVVSKDA
GKEASVDRVK AVRTLLISDE FSALDKAIDR FLTVLSELYK IDSKAFSEAT EVKGRTRVYF
ADNQETLIAS GKTTKPREIV GTPFWVITNT NTNRKRHMVE LLMERMGFQH DLTEKVCAAI
