SEQA_HAEIN
ID SEQA_HAEIN Reviewed; 197 AA.
AC P44564;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Negative modulator of initiation of replication {ECO:0000255|HAMAP-Rule:MF_00908};
GN Name=seqA {ECO:0000255|HAMAP-Rule:MF_00908}; OrderedLocusNames=HI_0192;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: Negative regulator of replication initiation, which
CC contributes to regulation of DNA replication and ensures that
CC replication initiation occurs exactly once per chromosome per cell
CC cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC region, thus preventing assembly of replication proteins and re-
CC initiation at newly replicated origins. Repression is relieved when the
CC region becomes fully methylated. {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000255|HAMAP-
CC Rule:MF_00908}.
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DR EMBL; L42023; AAC21861.1; -; Genomic_DNA.
DR PIR; D64053; D64053.
DR RefSeq; NP_438362.1; NC_000907.1.
DR RefSeq; WP_005648714.1; NC_000907.1.
DR AlphaFoldDB; P44564; -.
DR SMR; P44564; -.
DR STRING; 71421.HI_0192; -.
DR EnsemblBacteria; AAC21861; AAC21861; HI_0192.
DR KEGG; hin:HI_0192; -.
DR PATRIC; fig|71421.8.peg.198; -.
DR eggNOG; COG3057; Bacteria.
DR HOGENOM; CLU_099733_0_0_6; -.
DR OMA; RTRTYFA; -.
DR PhylomeDB; P44564; -.
DR BioCyc; HINF71421:G1GJ1-204-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.1220.10; -; 1.
DR Gene3D; 1.20.1380.10; -; 1.
DR HAMAP; MF_00908; SeqA; 1.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR010985; Ribbon_hlx_hlx.
DR InterPro; IPR005621; SeqA.
DR InterPro; IPR026577; SeqA_DNA-bd_C.
DR InterPro; IPR036835; SeqA_DNA-bd_C_sf.
DR InterPro; IPR033761; SeqA_N.
DR Pfam; PF03925; SeqA; 1.
DR Pfam; PF17206; SeqA_N; 1.
DR PIRSF; PIRSF019401; SeqA; 1.
DR SUPFAM; SSF47598; SSF47598; 1.
DR SUPFAM; SSF82808; SSF82808; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication inhibitor; DNA-binding; Reference proteome.
FT CHAIN 1..197
FT /note="Negative modulator of initiation of replication"
FT /id="PRO_0000097687"
FT REGION 100..101
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
FT REGION 129..133
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
FT REGION 163..169
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
SQ SEQUENCE 197 AA; 22381 MW; 0251E5AF885F30D8 CRC64;
MKIIEVDEEL YQYIASQTRS IGESASDILR RLLSLPVHTS IVNDLIITSA ETDQKPKQAI
NVKEVNIKTT KKQSITAINQ IVEKVQTLLN STEFQEESKA VVRFLAILRV LYRTNPESFA
QATESLQGRT RVYFARDEAT LLMAGNHTKP KQIPDTPYWV ITNTNSGRKM LMLEGAMQSM
ELPETLIDEV RSYFTVN
