SEQA_HAEP3
ID SEQA_HAEP3 Reviewed; 213 AA.
AC E1W6L8;
DT 16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=Negative modulator of initiation of replication {ECO:0000255|HAMAP-Rule:MF_00908};
GN Name=seqA {ECO:0000255|HAMAP-Rule:MF_00908}; OrderedLocusNames=PARA_19120;
OS Haemophilus parainfluenzae (strain T3T1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=862965;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T3T1;
RA Crook D., Hood D., Moxon R., Parkhill J., Aslett M., Bentley S.D.;
RT "The genome sequence of Haemophilus parainfluenzae T3T1.";
RL Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Negative regulator of replication initiation, which
CC contributes to regulation of DNA replication and ensures that
CC replication initiation occurs exactly once per chromosome per cell
CC cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC region, thus preventing assembly of replication proteins and re-
CC initiation at newly replicated origins. Repression is relieved when the
CC region becomes fully methylated. {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000255|HAMAP-
CC Rule:MF_00908}.
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DR EMBL; FQ312002; CBW16012.1; -; Genomic_DNA.
DR RefSeq; WP_014065626.1; NC_015964.1.
DR AlphaFoldDB; E1W6L8; -.
DR SMR; E1W6L8; -.
DR STRING; 862965.PARA_19120; -.
DR EnsemblBacteria; CBW16012; CBW16012; PARA_19120.
DR KEGG; hpr:PARA_19120; -.
DR PATRIC; fig|862965.3.peg.1904; -.
DR eggNOG; COG3057; Bacteria.
DR HOGENOM; CLU_099733_0_0_6; -.
DR OMA; RTRTYFA; -.
DR OrthoDB; 1625515at2; -.
DR Proteomes; UP000007052; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.1220.10; -; 1.
DR Gene3D; 1.20.1380.10; -; 1.
DR HAMAP; MF_00908; SeqA; 1.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR010985; Ribbon_hlx_hlx.
DR InterPro; IPR005621; SeqA.
DR InterPro; IPR026577; SeqA_DNA-bd_C.
DR InterPro; IPR036835; SeqA_DNA-bd_C_sf.
DR InterPro; IPR033761; SeqA_N.
DR Pfam; PF03925; SeqA; 1.
DR Pfam; PF17206; SeqA_N; 1.
DR PIRSF; PIRSF019401; SeqA; 1.
DR SUPFAM; SSF47598; SSF47598; 1.
DR SUPFAM; SSF82808; SSF82808; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication inhibitor; DNA-binding.
FT CHAIN 1..213
FT /note="Negative modulator of initiation of replication"
FT /id="PRO_0000413923"
FT REGION 116..117
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
FT REGION 145..149
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
FT REGION 179..185
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
SQ SEQUENCE 213 AA; 23946 MW; D650E3036997255E CRC64;
MKIIEVDEEL YQYIAAQTQS IGESASDILR RLLNLPTHAT SSVDFFESVA SAETPKSAVH
AEPVLTEKTT EVSQPKVEPV EPPKAVKKQS DEAINHIVDK VRALLNSAEF KEEPKAVVRF
LSILRTLYRT NPESFAQATE SLQGRTRVYF ARDEGTLLVA GNHTKPKQIP DTPYWVITNT
NSGRKMLMLE GAMQSMHLPE YLIDEVRPYF VSN
