ID   SEQA_HAMD5              Reviewed;         176 AA.
AC   C4K392;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Negative modulator of initiation of replication {ECO:0000255|HAMAP-Rule:MF_00908};
GN   Name=seqA {ECO:0000255|HAMAP-Rule:MF_00908}; OrderedLocusNames=HDEF_0270;
OS   Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; aphid secondary symbionts; Candidatus Hamiltonella.
OX   NCBI_TaxID=572265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=5AT;
RX   PubMed=19451630; DOI=10.1073/pnas.0900194106;
RA   Degnan P.H., Yu Y., Sisneros N., Wing R.A., Moran N.A.;
RT   "Hamiltonella defensa, genome evolution of protective bacterial
RT   endosymbiont from pathogenic ancestors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:9063-9068(2009).
CC   -!- FUNCTION: Negative regulator of replication initiation, which
CC       contributes to regulation of DNA replication and ensures that
CC       replication initiation occurs exactly once per chromosome per cell
CC       cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC       region, thus preventing assembly of replication proteins and re-
CC       initiation at newly replicated origins. Repression is relieved when the
CC       region becomes fully methylated. {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC       {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00908}.
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DR   EMBL; CP001277; ACQ67035.1; -; Genomic_DNA.
DR   RefSeq; WP_012737998.1; NC_012751.1.
DR   AlphaFoldDB; C4K392; -.
DR   SMR; C4K392; -.
DR   STRING; 572265.HDEF_0270; -.
DR   EnsemblBacteria; ACQ67035; ACQ67035; HDEF_0270.
DR   GeneID; 66260186; -.
DR   KEGG; hde:HDEF_0270; -.
DR   eggNOG; COG3057; Bacteria.
DR   HOGENOM; CLU_099733_0_0_6; -.
DR   OMA; RTRTYFA; -.
DR   Proteomes; UP000002334; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   Gene3D; 1.20.1380.10; -; 1.
DR   HAMAP; MF_00908; SeqA; 1.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   InterPro; IPR005621; SeqA.
DR   InterPro; IPR026577; SeqA_DNA-bd_C.
DR   InterPro; IPR036835; SeqA_DNA-bd_C_sf.
DR   InterPro; IPR033761; SeqA_N.
DR   Pfam; PF03925; SeqA; 1.
DR   Pfam; PF17206; SeqA_N; 1.
DR   PIRSF; PIRSF019401; SeqA; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
DR   SUPFAM; SSF82808; SSF82808; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication inhibitor; DNA-binding; Reference proteome.
FT   CHAIN           1..176
FT                   /note="Negative modulator of initiation of replication"
FT                   /id="PRO_0000413925"
SQ   SEQUENCE   176 AA;  20300 MW;  43D5AD26D465B032 CRC64;
     MKLIEVDEEL YRYIAAHTQY IGESASEILR RMFSLTQKKQ TEIKIKKTPK IHQSSVKATL
     FSRIQILNDI LSSETYASKN KVIARFMLIL STLYDLDSKA FSVAAESLQG RKRAYFSGSK
     NFLLKNGTHT KPKQIPKTPY WVITNTNSER KRTMLKNMMI AMKFPNEIAK KVSQTI