ID   SEQA_MANSM              Reviewed;         215 AA.
AC   Q65U90;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   16-NOV-2011, sequence version 2.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Negative modulator of initiation of replication {ECO:0000255|HAMAP-Rule:MF_00908};
GN   Name=seqA {ECO:0000255|HAMAP-Rule:MF_00908}; OrderedLocusNames=MS0863;
OS   Mannheimia succiniciproducens (strain MBEL55E).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Basfia.
OX   NCBI_TaxID=221988;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MBEL55E;
RX   PubMed=15378067; DOI=10.1038/nbt1010;
RA   Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H.,
RA   Jeong H., Hur C.G., Kim J.J.;
RT   "The genome sequence of the capnophilic rumen bacterium Mannheimia
RT   succiniciproducens.";
RL   Nat. Biotechnol. 22:1275-1281(2004).
CC   -!- FUNCTION: Negative regulator of replication initiation, which
CC       contributes to regulation of DNA replication and ensures that
CC       replication initiation occurs exactly once per chromosome per cell
CC       cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC       region, thus preventing assembly of replication proteins and re-
CC       initiation at newly replicated origins. Repression is relieved when the
CC       region becomes fully methylated. {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC       {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00908}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAU37470.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE016827; AAU37470.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_011200041.1; NC_006300.1.
DR   AlphaFoldDB; Q65U90; -.
DR   SMR; Q65U90; -.
DR   STRING; 221988.MS0863; -.
DR   EnsemblBacteria; AAU37470; AAU37470; MS0863.
DR   KEGG; msu:MS0863; -.
DR   eggNOG; COG3057; Bacteria.
DR   HOGENOM; CLU_099733_0_0_6; -.
DR   OrthoDB; 1625515at2; -.
DR   Proteomes; UP000000607; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   Gene3D; 1.20.1380.10; -; 1.
DR   HAMAP; MF_00908; SeqA; 1.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   InterPro; IPR005621; SeqA.
DR   InterPro; IPR026577; SeqA_DNA-bd_C.
DR   InterPro; IPR036835; SeqA_DNA-bd_C_sf.
DR   InterPro; IPR033761; SeqA_N.
DR   Pfam; PF03925; SeqA; 1.
DR   Pfam; PF17206; SeqA_N; 1.
DR   PIRSF; PIRSF019401; SeqA; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
DR   SUPFAM; SSF82808; SSF82808; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication inhibitor; DNA-binding.
FT   CHAIN           1..215
FT                   /note="Negative modulator of initiation of replication"
FT                   /id="PRO_0000413927"
FT   REGION          71..93
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          181..187
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
FT   COMPBIAS        73..93
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   215 AA;  24428 MW;  04A874DEDA8033CE CRC64;
     MKIIEVDEEL YQYIASQTKS IGESASDILR RLLNLPVSGV NLTAVDLTQS TMNSTNEEKG
     TQLPAEKNVV AETPKPSSEQ EIRTPARKQS TQSIQHIVTK VKNLLQSEAF QEESKMVVRF
     LNILSVLYRT NPESFAQATE QETSQGRTRT YYARDEATLL AAGNHTKPRQ IPDTPYWVIT
     NTNSGRKMLM LERTMQFMEL PEELIDEVRP YFAVV