ID   SEQA_PANAA              Reviewed;         183 AA.
AC   F2EP55;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   16-NOV-2011, sequence version 2.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=Negative modulator of initiation of replication {ECO:0000255|HAMAP-Rule:MF_00908};
GN   Name=seqA {ECO:0000255|HAMAP-Rule:MF_00908}; OrderedLocusNames=PAJ_0475;
OS   Pantoea ananatis (strain AJ13355).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea.
OX   NCBI_TaxID=932677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AJ13355;
RX   PubMed=22159605; DOI=10.1007/s00253-011-3713-5;
RA   Hara Y., Kadotani N., Izui H., Katashkina J.I., Kuvaeva T.M.,
RA   Andreeva I.G., Golubeva L.I., Malko D.B., Makeev V.J., Mashko S.V.,
RA   Kozlov Y.I.;
RT   "The complete genome sequence of Pantoea ananatis AJ13355, an organism with
RT   great biotechnological potential.";
RL   Appl. Microbiol. Biotechnol. 93:331-341(2012).
CC   -!- FUNCTION: Negative regulator of replication initiation, which
CC       contributes to regulation of DNA replication and ensures that
CC       replication initiation occurs exactly once per chromosome per cell
CC       cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC       region, thus preventing assembly of replication proteins and re-
CC       initiation at newly replicated origins. Repression is relieved when the
CC       region becomes fully methylated. {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC       {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00908}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAK10555.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AP012032; BAK10555.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_019104980.1; NC_017531.2.
DR   AlphaFoldDB; F2EP55; -.
DR   SMR; F2EP55; -.
DR   STRING; 932677.PAJ_0475; -.
DR   EnsemblBacteria; BAK10555; BAK10555; PAJ_0475.
DR   KEGG; paj:PAJ_0475; -.
DR   PATRIC; fig|553.3.peg.3157; -.
DR   eggNOG; COG3057; Bacteria.
DR   HOGENOM; CLU_099733_0_0_6; -.
DR   BioCyc; PANA932677:PAJ_RS02630-MON; -.
DR   Proteomes; UP000006690; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   Gene3D; 1.20.1380.10; -; 1.
DR   HAMAP; MF_00908; SeqA; 1.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   InterPro; IPR005621; SeqA.
DR   InterPro; IPR026577; SeqA_DNA-bd_C.
DR   InterPro; IPR036835; SeqA_DNA-bd_C_sf.
DR   InterPro; IPR033761; SeqA_N.
DR   Pfam; PF03925; SeqA; 1.
DR   Pfam; PF17206; SeqA_N; 1.
DR   PIRSF; PIRSF019401; SeqA; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
DR   SUPFAM; SSF82808; SSF82808; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication inhibitor; DNA-binding.
FT   CHAIN           1..183
FT                   /note="Negative modulator of initiation of replication"
FT                   /id="PRO_0000413928"
FT   REGION          43..70
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          89..90
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
FT   REGION          118..122
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
FT   REGION          152..158
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
SQ   SEQUENCE   183 AA;  20422 MW;  8A83323065D64D31 CRC64;
     MKTIEVDEEL YRYIASHTQH IGESASDILR RMLKFTAGQP AAVNDTQPVS APAPSKAAPS
     AGNESRPQDR VRAVRELMLS DEYAEQKRAV NRFMLILSTL YRLDTAAFAE ATTSLQGRTR
     IYFAGDEHTL LQSGTHTKPK HVPGTPYWVI TNTNTGRKRS MVEHIMLSMQ FPSELAEKVC
     GTI