ID   SEQA_PSET1              Reviewed;         197 AA.
AC   Q3IGV1;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Negative modulator of initiation of replication {ECO:0000255|HAMAP-Rule:MF_00908};
GN   Name=seqA {ECO:0000255|HAMAP-Rule:MF_00908}; OrderedLocusNames=PSHAa1635;
OS   Pseudoalteromonas translucida (strain TAC 125).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Pseudoalteromonadaceae; Pseudoalteromonas.
OX   NCBI_TaxID=326442;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TAC 125;
RX   PubMed=16169927; DOI=10.1101/gr.4126905;
RA   Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N.,
RA   Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C.,
RA   Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C., Rouy Z.,
RA   Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A.;
RT   "Coping with cold: the genome of the versatile marine Antarctica bacterium
RT   Pseudoalteromonas haloplanktis TAC125.";
RL   Genome Res. 15:1325-1335(2005).
CC   -!- FUNCTION: Negative regulator of replication initiation, which
CC       contributes to regulation of DNA replication and ensures that
CC       replication initiation occurs exactly once per chromosome per cell
CC       cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC       region, thus preventing assembly of replication proteins and re-
CC       initiation at newly replicated origins. Repression is relieved when the
CC       region becomes fully methylated. {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC       {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00908}.
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DR   EMBL; CR954246; CAI86708.1; -; Genomic_DNA.
DR   RefSeq; WP_011328313.1; NC_007481.1.
DR   AlphaFoldDB; Q3IGV1; -.
DR   SMR; Q3IGV1; -.
DR   STRING; 326442.PSHAa1635; -.
DR   EnsemblBacteria; CAI86708; CAI86708; PSHAa1635.
DR   KEGG; pha:PSHAa1635; -.
DR   PATRIC; fig|326442.8.peg.1582; -.
DR   eggNOG; COG3057; Bacteria.
DR   HOGENOM; CLU_099733_0_0_6; -.
DR   OMA; RTRTYFA; -.
DR   OrthoDB; 1625515at2; -.
DR   BioCyc; PHAL326442:PSHA_RS08020-MON; -.
DR   Proteomes; UP000006843; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   Gene3D; 1.20.1380.10; -; 1.
DR   HAMAP; MF_00908; SeqA; 1.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   InterPro; IPR005621; SeqA.
DR   InterPro; IPR026577; SeqA_DNA-bd_C.
DR   InterPro; IPR036835; SeqA_DNA-bd_C_sf.
DR   InterPro; IPR033761; SeqA_N.
DR   Pfam; PF03925; SeqA; 1.
DR   Pfam; PF17206; SeqA_N; 1.
DR   PIRSF; PIRSF019401; SeqA; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
DR   SUPFAM; SSF82808; SSF82808; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication inhibitor; DNA-binding; Reference proteome.
FT   CHAIN           1..197
FT                   /note="Negative modulator of initiation of replication"
FT                   /id="PRO_0000413932"
SQ   SEQUENCE   197 AA;  22157 MW;  C133F983F56616EC CRC64;
     MKQIDIDDEL YQYIASNTQS IGESASTILR RLLNLSGEKI QTANVELNQN NQTTTTLPKV
     PPVAEQTPAL AAKPSAVAEK SVQQKNANVF NVLNKEELAM QKGVVGRFLF ILSAFYRTHK
     TDFRAVLDIK GRDRVYFATS KEDLVNSGSS MNPKNITDSE YWVMTNSNTT RKKMMLHEVA
     LCLGYSTEQA EKIRDYL