SEQA_SHEDO
ID SEQA_SHEDO Reviewed; 253 AA.
AC Q12MQ7;
DT 16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Negative modulator of initiation of replication {ECO:0000255|HAMAP-Rule:MF_00908};
GN Name=seqA {ECO:0000255|HAMAP-Rule:MF_00908}; OrderedLocusNames=Sden_1986;
OS Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=318161;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OS217 / ATCC BAA-1090 / DSM 15013;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.;
RT "Complete sequence of Shewanella denitrificans OS217.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Negative regulator of replication initiation, which
CC contributes to regulation of DNA replication and ensures that
CC replication initiation occurs exactly once per chromosome per cell
CC cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC region, thus preventing assembly of replication proteins and re-
CC initiation at newly replicated origins. Repression is relieved when the
CC region becomes fully methylated. {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00908}.
CC -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000255|HAMAP-
CC Rule:MF_00908}.
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DR EMBL; CP000302; ABE55269.1; -; Genomic_DNA.
DR RefSeq; WP_011496425.1; NC_007954.1.
DR AlphaFoldDB; Q12MQ7; -.
DR SMR; Q12MQ7; -.
DR STRING; 318161.Sden_1986; -.
DR EnsemblBacteria; ABE55269; ABE55269; Sden_1986.
DR KEGG; sdn:Sden_1986; -.
DR eggNOG; COG3057; Bacteria.
DR HOGENOM; CLU_099733_0_0_6; -.
DR OMA; FEPNAID; -.
DR OrthoDB; 1625515at2; -.
DR Proteomes; UP000001982; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.1220.10; -; 1.
DR Gene3D; 1.20.1380.10; -; 1.
DR HAMAP; MF_00908; SeqA; 1.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR010985; Ribbon_hlx_hlx.
DR InterPro; IPR005621; SeqA.
DR InterPro; IPR026577; SeqA_DNA-bd_C.
DR InterPro; IPR036835; SeqA_DNA-bd_C_sf.
DR InterPro; IPR033761; SeqA_N.
DR Pfam; PF03925; SeqA; 1.
DR Pfam; PF17206; SeqA_N; 1.
DR SUPFAM; SSF47598; SSF47598; 1.
DR SUPFAM; SSF82808; SSF82808; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication inhibitor; DNA-binding; Reference proteome.
FT CHAIN 1..253
FT /note="Negative modulator of initiation of replication"
FT /id="PRO_0000413936"
FT REGION 66..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 154..155
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
SQ SEQUENCE 253 AA; 27382 MW; AE5411B944634457 CRC64;
MKYIEVDEEL YRFIASKTER IGESASDILR RLLTLPVDTV TPFEPNAIDE PSLDVAHHQA
SNFAASNQEQ QTGHGHAGEP SAVQTPESND YAKAQPHSSG YQPGQLEGHK SEPQTLAQLN
SQPLAVGQSS QSATSQVDFN ALVNEHLLSQ QKGAVGRFMW LLEGLAALAP SQFNKVLLVQ
GKGRLYFARS KDELLASSAS ANPKEIGTTG YWVTTNNNTA KKQAILVEVL TKLHCDETLA
SAIADRICDK VAG
