ID   SEQA_SHIFL              Reviewed;         181 AA.
AC   P0AFZ0; P36658;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Negative modulator of initiation of replication {ECO:0000255|HAMAP-Rule:MF_00908};
GN   Name=seqA {ECO:0000255|HAMAP-Rule:MF_00908};
GN   OrderedLocusNames=SF0606, S0617;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Negative regulator of replication initiation, which
CC       contributes to regulation of DNA replication and ensures that
CC       replication initiation occurs exactly once per chromosome per cell
CC       cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC       region, thus preventing assembly of replication proteins and re-
CC       initiation at newly replicated origins. Repression is relieved when the
CC       region becomes fully methylated. {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC       {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00908}.
CC   -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00908}.
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DR   EMBL; AE005674; AAN42244.2; -; Genomic_DNA.
DR   EMBL; AE014073; AAP16115.1; -; Genomic_DNA.
DR   RefSeq; NP_706537.2; NC_004337.2.
DR   RefSeq; WP_000848387.1; NZ_WPGW01000002.1.
DR   AlphaFoldDB; P0AFZ0; -.
DR   SMR; P0AFZ0; -.
DR   STRING; 198214.SF0606; -.
DR   EnsemblBacteria; AAN42244; AAN42244; SF0606.
DR   EnsemblBacteria; AAP16115; AAP16115; S0617.
DR   GeneID; 1023526; -.
DR   GeneID; 66671042; -.
DR   KEGG; sfl:SF0606; -.
DR   KEGG; sfx:S0617; -.
DR   PATRIC; fig|198214.7.peg.707; -.
DR   HOGENOM; CLU_099733_0_0_6; -.
DR   OMA; RTRTYFA; -.
DR   OrthoDB; 1625515at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   Gene3D; 1.20.1380.10; -; 1.
DR   HAMAP; MF_00908; SeqA; 1.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   InterPro; IPR005621; SeqA.
DR   InterPro; IPR026577; SeqA_DNA-bd_C.
DR   InterPro; IPR036835; SeqA_DNA-bd_C_sf.
DR   InterPro; IPR033761; SeqA_N.
DR   Pfam; PF03925; SeqA; 1.
DR   Pfam; PF17206; SeqA_N; 1.
DR   PIRSF; PIRSF019401; SeqA; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
DR   SUPFAM; SSF82808; SSF82808; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication inhibitor; DNA-binding; Reference proteome.
FT   CHAIN           1..181
FT                   /note="Negative modulator of initiation of replication"
FT                   /id="PRO_0000097686"
FT   REGION          87..88
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
FT   REGION          116..120
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
FT   REGION          150..156
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00908"
SQ   SEQUENCE   181 AA;  20315 MW;  37D9078CA3D7229B CRC64;
     MKTIEVDDEL YSYIASHTKH IGESASDILR RMLKFSAASQ PAAPVTKEVR VASPAIVEAK
     PVKTIKDKVR AMRELLLSDE YAEQKRAVNR FMLLLSTLYS LDAQAFAEAT ESLHGRTRVY
     FAADEQTLLK NGNQTKPKHV PGTPYWVITN TNTGRKCSMI EHIMQSMQFP AELIEKVCGT
     I