SER2_CAEEL
ID SER2_CAEEL Reviewed; 455 AA.
AC O02213; Q6LA87; Q6LA88; Q6LA89; Q6LA90;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 4.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Tyramine receptor Ser-2;
GN Name=ser-2; ORFNames=C02D4.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 12-455 (ISOFORMS A/B AND E/G), FUNCTION, AND
RP ALTERNATIVE SPLICING.
RX PubMed=12354282; DOI=10.1046/j.1471-4159.2002.01065.x;
RA Rex E., Komuniecki R.W.;
RT "Characterization of a tyramine receptor from Caenorhabditis elegans.";
RL J. Neurochem. 82:1352-1359(2002).
RN [3]
RP ALTERNATIVE PROMOTER USAGE, ALTERNATIVE SPLICING, TISSUE SPECIFICITY,
RP MUTAGENESIS OF ASP-155 AND ALA-368, AND DISRUPTION PHENOTYPE.
RX PubMed=14568548; DOI=10.1016/s0012-1606(03)00447-0;
RA Tsalik E.L., Niacaris T., Wenick A.S., Pau K., Avery L., Hobert O.;
RT "LIM homeobox gene-dependent expression of biogenic amine receptors in
RT restricted regions of the C. elegans nervous system.";
RL Dev. Biol. 263:81-102(2003).
CC -!- FUNCTION: G-protein coupled receptor for tyramine, a known
CC neurotransmitter and neuromodulator and direct precursor of octopamine.
CC The rank order of potency is tyramine > octopamine > dopamine >
CC serotonin > epinephrine = norepinephrine.
CC {ECO:0000269|PubMed:12354282}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative promoter usage, Alternative splicing; Named isoforms=6;
CC Comment=Additional isoforms seem to exist.;
CC Name=e;
CC IsoId=O02213-5; Sequence=Displayed;
CC Name=a;
CC IsoId=O02213-1; Sequence=VSP_001920;
CC Name=b;
CC IsoId=O02213-2; Sequence=VSP_011787, VSP_001920;
CC Name=c;
CC IsoId=O02213-3; Sequence=VSP_011787, VSP_011789, VSP_011790;
CC Name=d;
CC IsoId=O02213-4; Sequence=VSP_011788, VSP_011789, VSP_011790;
CC Name=g;
CC IsoId=O02213-6; Sequence=VSP_011787;
CC -!- TISSUE SPECIFICITY: The different isoforms are expressed in specific,
CC but overlapping sets of sensory, inter- and motor neurons, including
CC AIY, AIZ and RIA interneurons. They are also expressed in pharyngeal
CC cells, head muscles and excretory gland cells.
CC {ECO:0000269|PubMed:14568548}.
CC -!- DISRUPTION PHENOTYPE: A loss-of-function mutation does not affect the
CC development of AIY interneurons, or lead to egg-laying or any other
CC behavioral defects. {ECO:0000269|PubMed:14568548}.
CC -!- MISCELLANEOUS: [Isoform e]: Produced by alternative promoter usage.
CC -!- MISCELLANEOUS: [Isoform a]: Produced by alternative splicing of isoform
CC e. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform b]: Produced by alternative splicing of isoform
CC g. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform c]: Produced by alternative splicing of isoform
CC g. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform d]: Produced by alternative promoter usage.
CC {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform g]: Produced by alternative promoter usage.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z81031; CAB02718.4; -; Genomic_DNA.
DR EMBL; Z81031; CAF31464.1; -; Genomic_DNA.
DR EMBL; Z81031; CAF31465.2; -; Genomic_DNA.
DR EMBL; Z81031; CAF31466.2; -; Genomic_DNA.
DR EMBL; Z81031; CAF31467.1; -; Genomic_DNA.
DR EMBL; AF539813; AAN16458.1; -; mRNA.
DR EMBL; AF539814; AAN16459.1; -; mRNA.
DR PIR; T18863; T18863.
DR RefSeq; NP_001024335.1; NM_001029164.1. [O02213-1]
DR RefSeq; NP_001024336.1; NM_001029165.1. [O02213-2]
DR RefSeq; NP_001024337.1; NM_001029166.1. [O02213-3]
DR RefSeq; NP_001024338.1; NM_001029167.1. [O02213-4]
DR RefSeq; NP_001024339.1; NM_001029168.1. [O02213-5]
DR AlphaFoldDB; O02213; -.
DR SMR; O02213; -.
DR STRING; 6239.C02D4.2e; -.
DR PaxDb; O02213; -.
DR PRIDE; O02213; -.
DR EnsemblMetazoa; C02D4.2a.1; C02D4.2a.1; WBGene00004777. [O02213-1]
DR EnsemblMetazoa; C02D4.2b.1; C02D4.2b.1; WBGene00004777. [O02213-2]
DR EnsemblMetazoa; C02D4.2c.1; C02D4.2c.1; WBGene00004777. [O02213-3]
DR EnsemblMetazoa; C02D4.2d.1; C02D4.2d.1; WBGene00004777. [O02213-4]
DR EnsemblMetazoa; C02D4.2e.1; C02D4.2e.1; WBGene00004777. [O02213-5]
DR GeneID; 182110; -.
DR KEGG; cel:CELE_C02D4.2; -.
DR UCSC; C02D4.2f; c. elegans. [O02213-1]
DR CTD; 182110; -.
DR WormBase; C02D4.2a; CE36088; WBGene00004777; ser-2. [O02213-1]
DR WormBase; C02D4.2b; CE36089; WBGene00004777; ser-2. [O02213-2]
DR WormBase; C02D4.2c; CE36423; WBGene00004777; ser-2. [O02213-3]
DR WormBase; C02D4.2d; CE36424; WBGene00004777; ser-2. [O02213-4]
DR WormBase; C02D4.2e; CE36092; WBGene00004777; ser-2. [O02213-5]
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000165278; -.
DR InParanoid; O02213; -.
DR OMA; MKYHNNG; -.
DR OrthoDB; 737211at2759; -.
DR PhylomeDB; O02213; -.
DR Reactome; R-CEL-390696; Adrenoceptors.
DR Reactome; R-CEL-392023; Adrenaline signalling through Alpha-2 adrenergic receptor.
DR Reactome; R-CEL-400042; Adrenaline,noradrenaline inhibits insulin secretion.
DR Reactome; R-CEL-418594; G alpha (i) signalling events.
DR Reactome; R-CEL-418597; G alpha (z) signalling events.
DR PRO; PR:O02213; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00004777; Expressed in larva and 3 other tissues.
DR ExpressionAtlas; O02213; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:WormBase.
DR GO; GO:0008227; F:G protein-coupled amine receptor activity; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0004989; F:octopamine receptor activity; IEA:InterPro.
DR GO; GO:0008226; F:tyramine receptor activity; IDA:UniProtKB.
DR GO; GO:0071880; P:adenylate cyclase-activating adrenergic receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007212; P:dopamine receptor signaling pathway; ISS:WormBase.
DR GO; GO:0007211; P:octopamine or tyramine signaling pathway; IDA:UniProtKB.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR002002; Octopmn_rcpt.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00664; OCTOPAMINER.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Alternative promoter usage; Alternative splicing; Cell membrane;
KW Disulfide bond; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..455
FT /note="Tyramine receptor Ser-2"
FT /id="PRO_0000070108"
FT TOPO_DOM 1..60
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..83
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..93
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..115
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..133
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..174
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..197
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..221
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..243
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..370
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..392
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..407
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..428
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 429..455
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 4
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 26
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 131..209
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VAR_SEQ 1..10
FT /note="MFRNYTDSVQ -> MSSL (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_011788"
FT VAR_SEQ 1..9
FT /note="MFRNYTDSV -> MNDDFS (in isoform b, isoform c and
FT isoform g)"
FT /evidence="ECO:0000305"
FT /id="VSP_011787"
FT VAR_SEQ 182..202
FT /note="VWILSMLISVPPIIGWNNWQE -> SLQSNYGEGKRRRLPSFVMEM (in
FT isoform c and isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_011789"
FT VAR_SEQ 203..455
FT /note="Missing (in isoform c and isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_011790"
FT VAR_SEQ 288..310
FT /note="Missing (in isoform a and isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_001920"
FT MUTAGEN 155
FT /note="D->A: No obvious behavioral defects."
FT /evidence="ECO:0000269|PubMed:14568548"
FT MUTAGEN 368
FT /note="A->E: No obvious behavioral defects."
FT /evidence="ECO:0000269|PubMed:14568548"
FT CONFLICT 71
FT /note="T -> A (in Ref. 2; AAN16459)"
FT /evidence="ECO:0000305"
FT CONFLICT 268
FT /note="D -> V (in Ref. 2; AAN16459)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 455 AA; 50870 MW; A890DB8A9101E29F CRC64;
MFRNYTDSVQ EMVLRAIDSI RDSVINASSA VSTTTLPPLD IPMTSMKPPS IIPTVELVLG
TITYLVIIAM TVVGNTLVVV AVFSYRPLKK VQNYFLVSLA ASDLAVAIFV MPLHVVTFLA
GGKWLLGVTV CQFFTTADIL LCTSSILNLC AIALDRYWAI HNPINYAQKR TTKFVCIVIV
IVWILSMLIS VPPIIGWNNW QENMMEDSCG LSTEKAFVVF SAAGSFFLPL LVMVVVYVKI
FISARQRIRT NRGRSALMRI QNAEGDDDYR KMSIKRASVE SARTSSRVGE KTPLVIADGQ
TTVTTLAAHS TDGGSLPKDE TTKHMKYHNN GSCKVKVKDV KEDEGNPNPT AVLRKREKIS
VAKEKRAAKT IAVIIFVFSF CWLPFFVAYV IRPFCETCKL HAKVEQAFTW LGYINSSLNP
FLYGILNLEF RRAFKKILCP KAVLEQRRRR MSAQP
