BGL19_ARATH
ID BGL19_ARATH Reviewed; 527 AA.
AC Q9LIF9; Q93Z07;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Beta-glucosidase 19;
DE Short=AtBGLU19;
DE EC=3.2.1.21;
DE Flags: Precursor;
GN Name=BGLU19; OrderedLocusNames=At3g21370; ORFNames=MHC9.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15604686; DOI=10.1007/s11103-004-0790-1;
RA Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R.,
RA Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.;
RT "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase
RT family 1.";
RL Plant Mol. Biol. 55:343-367(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC with release of beta-D-glucose.; EC=3.2.1.21;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000255|PROSITE-
CC ProRule:PRU10138}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
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DR EMBL; AP001305; BAB03050.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76501.1; -; Genomic_DNA.
DR EMBL; AY058865; AAL24252.1; -; mRNA.
DR EMBL; AY064046; AAL36402.1; -; mRNA.
DR EMBL; AY096383; AAM20024.1; -; mRNA.
DR RefSeq; NP_188774.2; NM_113032.4.
DR AlphaFoldDB; Q9LIF9; -.
DR SMR; Q9LIF9; -.
DR BioGRID; 7023; 2.
DR IntAct; Q9LIF9; 1.
DR STRING; 3702.AT3G21370.1; -.
DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR iPTMnet; Q9LIF9; -.
DR PaxDb; Q9LIF9; -.
DR PRIDE; Q9LIF9; -.
DR ProteomicsDB; 240395; -.
DR EnsemblPlants; AT3G21370.1; AT3G21370.1; AT3G21370.
DR GeneID; 821691; -.
DR Gramene; AT3G21370.1; AT3G21370.1; AT3G21370.
DR KEGG; ath:AT3G21370; -.
DR Araport; AT3G21370; -.
DR TAIR; locus:2089433; AT3G21370.
DR eggNOG; KOG0626; Eukaryota.
DR HOGENOM; CLU_001859_1_0_1; -.
DR InParanoid; Q9LIF9; -.
DR OMA; QPNTAKM; -.
DR OrthoDB; 408001at2759; -.
DR PhylomeDB; Q9LIF9; -.
DR BioCyc; ARA:AT3G21370-MON; -.
DR PRO; PR:Q9LIF9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LIF9; baseline and differential.
DR Genevisible; Q9LIF9; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0019762; P:glucosinolate catabolic process; IBA:GO_Central.
DR GO; GO:0009651; P:response to salt stress; IBA:GO_Central.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; PTHR10353; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00014; ER_TARGET; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Endoplasmic reticulum; Glycoprotein; Glycosidase;
KW Hydrolase; Reference proteome; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..527
FT /note="Beta-glucosidase 19"
FT /id="PRO_0000389581"
FT MOTIF 524..527
FT /note="Prevents secretion from ER"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10138"
FT ACT_SITE 201
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 418
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 52
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 155
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 200
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 345
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 469
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 476..477
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 462
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 495
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 220..231
FT /evidence="ECO:0000250"
FT CONFLICT 349
FT /note="F -> L (in Ref. 3; AAL24252)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 527 AA; 60015 MW; A9EB34BC818BF88A CRC64;
MKIPLLGLLL LISLVGSPTR AEEGPVCPKT ETLSRASFPE GFMFGTATAA FQVEGAVNEG
CRGPSLWDIY TKKFPHRVKN HNADEAVDFY HRYKEDIQLM KKLNTDGFRL SISWPRIFPH
GRMEKGISKE GVQFYHDLID ELLKNDITPL VTVFHWDTPA DLEDEYGGFL SERIVPDFVE
YANFTFHEYG DKVKNWITFN EPWVFSRSGY DVGKKAPGRC SPYVKEFGKL CQDGRSGFEP
YVVSHNLLVG HAEAVDAFRK CEKCKGGKIG IAHSPAWFEP EDVEGGQATV NRVLDFVIGW
HLDPTTFGDY PQSMKDAVGS RLPRFTKAQK AKLKDSTDFV GINYYTSFFA KADQKVDSRN
PTWATDALVE FEPKTVDGSI KIGSQPNTAK MAVYAKGLRK LMKYIKDRYN SPEIIITENG
YGEDLGDKDT DLSVALNDHN RKYYLQRHLL ALNEAICEDK VNVTSYFLWS LMDNFEWQDG
YTARFGVYYI DFKNNLTRME KESAKWLSEF LKPGLKPSKS SKLHEEL