SER3_DROME
ID SER3_DROME Reviewed; 272 AA.
AC P17207; Q9VAD8;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Serine protease 3;
DE EC=3.4.21.-;
DE AltName: Full=Protein Jonah 99Ci;
DE Flags: Precursor;
GN Name=Jon99Ci; Synonyms=SER3, Ser99Dc; ORFNames=CG17951;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 212-272, FUNCTION, TISSUE SPECIFICITY,
RP AND DEVELOPMENTAL STAGE.
RX PubMed=2469005; DOI=10.1128/mcb.9.2.692-700.1989;
RA Yun Y., Davis R.L.;
RT "Levels of RNA from a family of putative serine protease genes are reduced
RT in Drosophila melanogaster dunce mutants and are regulated by cyclic AMP.";
RL Mol. Cell. Biol. 9:692-700(1989).
CC -!- FUNCTION: Its major function may be to aid in digestion.
CC {ECO:0000269|PubMed:2469005}.
CC -!- TISSUE SPECIFICITY: Abundantly expressed in the larval gut.
CC {ECO:0000269|PubMed:2469005}.
CC -!- DEVELOPMENTAL STAGE: Began to appear at late embryo stage and continued
CC to increase in abundance throughout the larval stage. They are not
CC present in pupae but reappeared in the adult.
CC {ECO:0000269|PubMed:2469005}.
CC -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00274}.
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DR EMBL; AE014297; AAF56973.1; -; Genomic_DNA.
DR EMBL; AY070605; AAL48076.1; -; mRNA.
DR EMBL; M24380; AAB02551.1; -; Genomic_DNA.
DR PIR; PS0049; PS0049.
DR RefSeq; NP_524555.1; NM_079831.4.
DR AlphaFoldDB; P17207; -.
DR SMR; P17207; -.
DR STRING; 7227.FBpp0084877; -.
DR MEROPS; S01.B24; -.
DR PaxDb; P17207; -.
DR PRIDE; P17207; -.
DR DNASU; 43545; -.
DR EnsemblMetazoa; FBtr0085511; FBpp0084877; FBgn0003358.
DR GeneID; 43545; -.
DR KEGG; dme:Dmel_CG31039; -.
DR CTD; 43545; -.
DR FlyBase; FBgn0003358; Jon99Ci.
DR VEuPathDB; VectorBase:FBgn0003358; -.
DR eggNOG; KOG3627; Eukaryota.
DR GeneTree; ENSGT00840000130082; -.
DR HOGENOM; CLU_006842_7_6_1; -.
DR InParanoid; P17207; -.
DR OMA; AFIRYPE; -.
DR OrthoDB; 1522379at2759; -.
DR PhylomeDB; P17207; -.
DR BioGRID-ORCS; 43545; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43545; -.
DR PRO; PR:P17207; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0003358; Expressed in adult midgut (Drosophila) and 15 other tissues.
DR Genevisible; P17207; DM.
DR GO; GO:0004175; F:endopeptidase activity; ISM:FlyBase.
DR GO; GO:0017171; F:serine hydrolase activity; HDA:FlyBase.
DR GO; GO:0004252; F:serine-type endopeptidase activity; ISM:FlyBase.
DR GO; GO:0006508; P:proteolysis; ISM:FlyBase.
DR CDD; cd00190; Tryp_SPc; 1.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001314; Peptidase_S1A.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR InterPro; IPR033116; TRYPSIN_SER.
DR Pfam; PF00089; Trypsin; 1.
DR PRINTS; PR00722; CHYMOTRYPSIN.
DR SMART; SM00020; Tryp_SPc; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS50240; TRYPSIN_DOM; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
DR PROSITE; PS00135; TRYPSIN_SER; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Hydrolase; Protease; Reference proteome; Serine protease;
KW Signal; Zymogen.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT PROPEP 18..40
FT /evidence="ECO:0000250"
FT /id="PRO_0000028139"
FT CHAIN 41..272
FT /note="Serine protease 3"
FT /id="PRO_0000028140"
FT DOMAIN 41..269
FT /note="Peptidase S1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT ACT_SITE 84
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 127
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 222
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT DISULFID 69..85
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 193..208
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 218..246
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT CONFLICT 212
FT /note="P -> M (in Ref. 4; AAB02551)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 272 AA; 29620 MW; A61E9DE3AFCA93AF CRC64;
MRGLTLLSLA FLGVCSALTV PHSLVHPRDL EIRHGGIEGR ITNGNLASEG QVPYIVGVSL
NSNGNWWWCG GSIIGHTWVL TAAHCTAGAD EASLYYGAVN YNEPAFRHTV SSENFIRYPH
YVGLDHDLAL IKTPHVDFYS LVNKIELPSL DDRYNSYENN WVQAAGWGAI YDGSNVVEDL
RVVDLKVISV AECQAYYGTD TASENTICVE TPDGKATCQG DSGGPLVTKE GDKLIGITSF
VSAYGCQVGG PAGFTRVTKY LEWIKEETGI YY
