SERA_MANSE
ID SERA_MANSE Reviewed; 392 AA.
AC P14754;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Alaserpin;
DE AltName: Full=Serpin-1;
DE Flags: Precursor;
OS Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC Sphingidae; Sphinginae; Sphingini; Manduca.
OX NCBI_TaxID=7130;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Hemolymph;
RX PubMed=2463253; DOI=10.1016/s0021-9258(19)85038-x;
RA Kanost M.R., Prasad S.V., Wells M.A.;
RT "Primary structure of a member of the serpin superfamily of proteinase
RT inhibitors from an insect, Manduca sexta.";
RL J. Biol. Chem. 264:965-972(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Hemocyte;
RX PubMed=8276850; DOI=10.1016/s0021-9258(17)42311-8;
RA Jiang H., Kanost M.R.;
RT "Mutually exclusive exon use and reactive center diversity in insect
RT serpins.";
RL J. Biol. Chem. 269:55-58(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8910411; DOI=10.1074/jbc.271.45.28017;
RA Jiang H., Wang Y., Huang Y., Mulnix A.B., Kadel J., Cole K., Kanost M.R.;
RT "Organization of serpin gene-1 from Manduca sexta. Evolution of a family of
RT alternate exons encoding the reactive site loop.";
RL J. Biol. Chem. 271:28017-28023(1996).
RN [4]
RP DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=14728663; DOI=10.1111/j.1365-2583.2004.00454.x;
RA Gorman M.J., Kankanala P., Kanost M.R.;
RT "Bacterial challenge stimulates innate immune responses in extra-embryonic
RT tissues of tobacco hornworm eggs.";
RL Insect Mol. Biol. 13:19-24(2004).
CC -!- FUNCTION: Inhibits elastase.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced.;
CC Name=1;
CC IsoId=P14754-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Hemolymph.
CC -!- DEVELOPMENTAL STAGE: Expressed in naive 2 h (precellular blastoderm
CC stage) and 24 h (predorsal closure stage) eggs.
CC {ECO:0000269|PubMed:14728663}.
CC -!- INDUCTION: By bacterial infection. {ECO:0000269|PubMed:14728663}.
CC -!- SIMILARITY: Belongs to the serpin family. {ECO:0000305}.
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DR EMBL; M23438; AAA29327.1; -; mRNA.
DR EMBL; L20792; AAA29329.1; -; mRNA.
DR EMBL; U58361; AAC47343.1; -; Genomic_DNA.
DR PIR; A38829; A38829.
DR PDB; 1K9O; X-ray; 2.30 A; I=15-392.
DR PDB; 1SEK; X-ray; 2.10 A; A=15-354.
DR PDBsum; 1K9O; -.
DR PDBsum; 1SEK; -.
DR AlphaFoldDB; P14754; -.
DR SMR; P14754; -.
DR IntAct; P14754; 1.
DR MINT; P14754; -.
DR MEROPS; I04.031; -.
DR EvolutionaryTrace; P14754; -.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0009617; P:response to bacterium; IEP:UniProtKB.
DR Gene3D; 2.30.39.10; -; 1.
DR Gene3D; 3.30.497.10; -; 1.
DR InterPro; IPR015557; Serpin_B1.
DR InterPro; IPR023795; Serpin_CS.
DR InterPro; IPR023796; Serpin_dom.
DR InterPro; IPR000215; Serpin_fam.
DR InterPro; IPR036186; Serpin_sf.
DR InterPro; IPR042178; Serpin_sf_1.
DR InterPro; IPR042185; Serpin_sf_2.
DR PANTHER; PTHR11461; PTHR11461; 1.
DR PANTHER; PTHR11461:SF180; PTHR11461:SF180; 1.
DR Pfam; PF00079; Serpin; 1.
DR SMART; SM00093; SERPIN; 1.
DR SUPFAM; SSF56574; SSF56574; 1.
DR PROSITE; PS00284; SERPIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Glycoprotein; Protease inhibitor;
KW Secreted; Serine protease inhibitor; Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..392
FT /note="Alaserpin"
FT /id="PRO_0000032527"
FT SITE 359..360
FT /note="Reactive bond"
FT /evidence="ECO:0000250"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT HELIX 19..22
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 25..41
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 54..56
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 57..63
FT /evidence="ECO:0007829|PDB:1K9O"
FT TURN 64..66
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 70..78
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 86..94
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 102..104
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 107..117
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 123..125
FT /evidence="ECO:0007829|PDB:1K9O"
FT TURN 127..129
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 130..132
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 136..139
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 144..158
FT /evidence="ECO:0007829|PDB:1K9O"
FT TURN 159..161
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 169..171
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 178..188
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 190..192
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 196..198
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 200..204
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 206..209
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 212..228
FT /evidence="ECO:0007829|PDB:1K9O"
FT TURN 229..232
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 233..240
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 241..243
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 244..254
FT /evidence="ECO:0007829|PDB:1K9O"
FT TURN 255..257
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 258..264
FT /evidence="ECO:0007829|PDB:1K9O"
FT TURN 268..276
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 279..288
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 290..297
FT /evidence="ECO:0007829|PDB:1K9O"
FT HELIX 298..303
FT /evidence="ECO:0007829|PDB:1K9O"
FT TURN 304..306
FT /evidence="ECO:0007829|PDB:1K9O"
FT TURN 313..315
FT /evidence="ECO:0007829|PDB:1K9O"
FT TURN 319..321
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 322..324
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 331..341
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 345..347
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 349..351
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 354..359
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 366..368
FT /evidence="ECO:0007829|PDB:1K9O"
FT STRAND 373..390
FT /evidence="ECO:0007829|PDB:1K9O"
SQ SEQUENCE 392 AA; 43510 MW; 5138617BDE7EDA7A CRC64;
MKIIMCIFGL AALAMAGETD LQKILRESND QFTAQMFSEV VKANPGQNVV LSAFSVLPPL
GQLALASVGE SHDELLRALA LPNDNVTKDV FADLNRGVRA VKGVDLKMAS KIYVAKGLEL
NDDFAAVSRD VFGSEVQNVD FVKSVEAAGA INKWVEDQTN NRIKNLVDPD ALDETTRSVL
VNAIYFKGSW KDKFVKERTM DRDFHVSKDK TIKVPTMIGK KDVRYADVPE LDAKMIEMSY
EGDQASMIII LPNQVDGITA LEQKLKDPKA LSRAEERLYN TEVEIYLPKF KIETTTDLKE
VLSNMNIKKL FTPGAARLEN LLKTKESLYV DAAIQKAFIE VNEEGAEAAA ANAFGIVPAS
LILYPEVHID RPFYFELKID GIPMFNGKVI EP
