BGL1_ASPAC
ID BGL1_ASPAC Reviewed; 860 AA.
AC P48825;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Beta-glucosidase 1;
DE EC=3.2.1.21;
DE AltName: Full=Beta-D-glucoside glucohydrolase;
DE AltName: Full=Cellobiase;
DE AltName: Full=Gentiobiase;
DE Flags: Precursor;
OS Aspergillus aculeatus.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=5053;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=F-50;
RX PubMed=8964516; DOI=10.1016/0378-1119(96)00179-5;
RA Kawaguchi T., Enoki T., Tsurumaki S., Sumitani J., Ueda M., Ooi T.,
RA Arai M.;
RT "Cloning and sequencing of the cDNA encoding beta-glucosidase 1 from
RT Aspergillus aculeatus.";
RL Gene 173:287-288(1996).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC with release of beta-D-glucose.; EC=3.2.1.21;
CC -!- PATHWAY: Glycan metabolism; cellulose degradation.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 3 family. {ECO:0000305}.
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DR EMBL; D64088; BAA10968.1; -; mRNA.
DR PIR; JC4939; JC4939.
DR PDB; 4IIB; X-ray; 1.80 A; A/B=20-860.
DR PDB; 4IIC; X-ray; 1.90 A; A/B=20-860.
DR PDB; 4IID; X-ray; 2.30 A; A/B=20-860.
DR PDB; 4IIE; X-ray; 2.00 A; A/B=20-860.
DR PDB; 4IIF; X-ray; 2.45 A; A/B=20-860.
DR PDB; 4IIG; X-ray; 2.30 A; A/B=20-860.
DR PDB; 4IIH; X-ray; 2.00 A; A/B=20-860.
DR PDBsum; 4IIB; -.
DR PDBsum; 4IIC; -.
DR PDBsum; 4IID; -.
DR PDBsum; 4IIE; -.
DR PDBsum; 4IIF; -.
DR PDBsum; 4IIG; -.
DR PDBsum; 4IIH; -.
DR AlphaFoldDB; P48825; -.
DR SMR; P48825; -.
DR BindingDB; P48825; -.
DR ChEMBL; CHEMBL3493; -.
DR CAZy; GH3; Glycoside Hydrolase Family 3.
DR CLAE; BGL3A_ASPAC; -.
DR CLAE; BGL3A_ASPSA; -.
DR VEuPathDB; FungiDB:ASPACDRAFT_50312; -.
DR BRENDA; 3.2.1.21; 488.
DR UniPathway; UPA00696; -.
DR GO; GO:0008422; F:beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 3.20.20.300; -; 1.
DR Gene3D; 3.40.50.1700; -; 1.
DR InterPro; IPR026891; Fn3-like.
DR InterPro; IPR019800; Glyco_hydro_3_AS.
DR InterPro; IPR002772; Glyco_hydro_3_C.
DR InterPro; IPR036881; Glyco_hydro_3_C_sf.
DR InterPro; IPR001764; Glyco_hydro_3_N.
DR InterPro; IPR036962; Glyco_hydro_3_N_sf.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR Pfam; PF14310; Fn3-like; 1.
DR Pfam; PF00933; Glyco_hydro_3; 1.
DR Pfam; PF01915; Glyco_hydro_3_C; 1.
DR PRINTS; PR00133; GLHYDRLASE3.
DR SMART; SM01217; Fn3_like; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF52279; SSF52279; 1.
DR PROSITE; PS00775; GLYCOSYL_HYDROL_F3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Cellulose degradation; Glycoprotein;
KW Glycosidase; Hydrolase; Polysaccharide degradation; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..860
FT /note="Beta-glucosidase 1"
FT /id="PRO_0000011776"
FT ACT_SITE 280
FT /evidence="ECO:0000250"
FT CARBOHYD 61
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 211
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 252
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 315
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 322
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 354
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 387
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 442
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 523
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 542
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 564
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 658
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 668
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 690
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 712
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT HELIX 38..40
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 41..51
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 56..63
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 70..77
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 81..83
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 89..91
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 109..115
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 118..134
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 157..159
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 165..181
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 185..192
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 202..207
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 217..219
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 222..227
FT /evidence="ECO:0007829|PDB:4IIB"
FT TURN 228..230
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 231..238
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 243..246
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 248..251
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 256..258
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 260..263
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 264..270
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 275..279
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 288..293
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 297..304
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 314..321
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 327..343
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 346..348
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 362..367
FT /evidence="ECO:0007829|PDB:4IIB"
FT TURN 368..371
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 372..377
FT /evidence="ECO:0007829|PDB:4IIB"
FT TURN 386..388
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 389..398
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 401..407
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 416..422
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 423..425
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 436..438
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 449..452
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 462..472
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 476..480
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 486..495
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 497..505
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 517..520
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 530..540
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 542..552
FT /evidence="ECO:0007829|PDB:4IIB"
FT TURN 557..561
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 565..570
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 575..577
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 578..586
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 602..604
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 605..607
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 623..625
FT /evidence="ECO:0007829|PDB:4IIB"
FT TURN 627..630
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 634..639
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 657..666
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 691..694
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 711..714
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 716..720
FT /evidence="ECO:0007829|PDB:4IIB"
FT TURN 723..726
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 729..732
FT /evidence="ECO:0007829|PDB:4IIB"
FT TURN 735..738
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 750..754
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 756..759
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 761..771
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 773..775
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 777..779
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 782..786
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 796..800
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 803..805
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 810..818
FT /evidence="ECO:0007829|PDB:4IIB"
FT HELIX 819..822
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 824..826
FT /evidence="ECO:0007829|PDB:4IIB"
FT TURN 827..830
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 831..833
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 840..846
FT /evidence="ECO:0007829|PDB:4IIB"
FT STRAND 852..855
FT /evidence="ECO:0007829|PDB:4IIB"
SQ SEQUENCE 860 AA; 93053 MW; 4B484778B00FC694 CRC64;
MKLSWLEAAA LTAASVVSAD ELAFSPPFYP SPWANGQGEW AEAYQRAVAI VSQMTLDEKV
NLTTGTGWEL EKCVGQTGGV PRLNIGGMCL QDSPLGIRDS DYNSAFPAGV NVAATWDKNL
AYLRGQAMGQ EFSDKGIDVQ LGPAAGPLGR SPDGGRNWEG FSPDPALTGV LFAETIKGIQ
DAGVVATAKH YILNEQEHFR QVAEAAGYGF NISDTISSNV DDKTIHEMYL WPFADAVRAG
VGAIMCSYNQ INNSYGCQNS YTLNKLLKAE LGFQGFVMSD WGAHHSGVGS ALAGLDMSMP
GDITFDSATS FWGTNLTIAV LNGTVPQWRV DDMAVRIMAA YYKVGRDRLY QPPNFSSWTR
DEYGFKYFYP QEGPYEKVNH FVNVQRNHSE VIRKLGADST VLLKNNNALP LTGKERKVAI
LGEDAGSNSY GANGCSDRGC DNGTLAMAWG SGTAEFPYLV TPEQAIQAEV LKHKGSVYAI
TDNWALSQVE TLAKQASVSL VFVNSDAGEG YISVDGNEGD RNNLTLWKNG DNLIKAAANN
CNNTIVVIHS VGPVLVDEWY DHPNVTAILW AGLPGQESGN SLADVLYGRV NPGAKSPFTW
GKTREAYGDY LVRELNNGNG APQDDFSEGV FIDYRGFDKR NETPIYEFGH GLSYTTFNYS
GLHIQVLNAS SNAQVATETG AAPTFGQVGN ASDYVYPEGL TRISKFIYPW LNSTDLKASS
GDPYYGVDTA EHVPEGATDG SPQPVLPAGG GSGGNPRLYD ELIRVSVTVK NTGRVAGDAV
PQLYVSLGGP NEPKVVLRKF DRLTLKPSEE TVWTTTLTRR DLSNWDVAAQ DWVITSYPKK
VHVGSSSRQL PLHAALPKVQ
