ID   BGL22_ORYSJ             Reviewed;         533 AA.
AC   Q60DX8; A0A0P0WLG6;
DT   15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Beta-glucosidase 22;
DE            Short=Os5bglu22;
DE            EC=3.2.1.21;
DE   Flags: Precursor;
GN   Name=BGLU22; OrderedLocusNames=Os05g0366600, LOC_Os05g30350;
GN   ORFNames=OSJNBa0090H02.10;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA   Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17196101; DOI=10.1186/1471-2229-6-33;
RA   Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A.,
RA   Ketudat Cairns J.R.;
RT   "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12
RT   beta-glucosidase.";
RL   BMC Plant Biol. 6:33-33(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC         with release of beta-D-glucose.; EC=3.2.1.21;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
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DR   EMBL; AC137618; AAV31358.1; -; Genomic_DNA.
DR   EMBL; AP008211; BAF17245.1; -; Genomic_DNA.
DR   EMBL; AP014961; BAS93647.1; -; Genomic_DNA.
DR   EMBL; AK071469; BAG92509.1; -; mRNA.
DR   RefSeq; XP_015640028.1; XM_015784542.1.
DR   AlphaFoldDB; Q60DX8; -.
DR   SMR; Q60DX8; -.
DR   STRING; 4530.OS05T0366600-01; -.
DR   CAZy; GH1; Glycoside Hydrolase Family 1.
DR   PaxDb; Q60DX8; -.
DR   PRIDE; Q60DX8; -.
DR   EnsemblPlants; Os05t0366600-01; Os05t0366600-01; Os05g0366600.
DR   GeneID; 4338560; -.
DR   Gramene; Os05t0366600-01; Os05t0366600-01; Os05g0366600.
DR   KEGG; osa:4338560; -.
DR   eggNOG; KOG0626; Eukaryota.
DR   HOGENOM; CLU_001859_1_0_1; -.
DR   InParanoid; Q60DX8; -.
DR   OMA; MQWNHHK; -.
DR   OrthoDB; 408001at2759; -.
DR   Proteomes; UP000000763; Chromosome 5.
DR   Proteomes; UP000059680; Chromosome 5.
DR   Genevisible; Q60DX8; OS.
DR   GO; GO:0033907; F:beta-D-fucosidase activity; IEA:UniProt.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProt.
DR   GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR   GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353; PTHR10353; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 1.
DR   PRINTS; PR00131; GLHYDRLASE1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW   Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..533
FT                   /note="Beta-glucosidase 22"
FT                   /id="PRO_0000390339"
FT   ACT_SITE        207
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        421
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         61
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         161
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         350
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         466
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         473..474
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        41
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        233
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        238
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        435
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        226..234
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   533 AA;  59543 MW;  AEFE37457D9D166B CRC64;
     MAVSSSTSTC SSFSLLLLLL LLAAAPWRSG EAAAAAARAL NFTRQDFPGE FVFGAGTSAY
     QYEGATDEDG RSPSIWDTFT HAGKMPDKST GDMGAGGYHK YKEDVKLMSD TSLEAYRFSI
     SWSRLIPRGR GPVNPKGLEY YNSLIDELVE RGIEIHVTLY HLDFPQILED EYHGWLSPRV
     IDDFTAYADV CFREFGDRVR HWTTMDEPNV LSIAAYDSGA FPPCRCSPPF GANCTAGNST
     VEPYVVAHNS ILAHASVTRL YRDKYQATQE GFVGMNIYSF WNYPFSSSSA DIAATQRALD
     FMVGWILDPL VYGDYPEIMK KKAGSRIPSF TEEQSELIRG SADFIGINHY TSVYISDASN
     GETVGPRDYS ADMAATFRIS RNDTPSGQFV PTRLPRDPKG LQCMLEYLRD TYQGIPVYIQ
     ENGFGHFGKD DDSLNDTDRV DYLSSYMGST LAALRNGANV KGYFVWSFLD VFELLAGYHS
     PFGLHYVDFE DPNLPRQPKL SAHWYSKFLR GEIGINIEST ISPDEHEHEH ADQ