ID   BGL25_ORYSJ             Reviewed;         501 AA.
AC   Q0DA21; B7EF46;
DT   15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 2.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Beta-glucosidase 25;
DE            Short=Os6bglu25;
DE            EC=3.2.1.21;
DE   Flags: Precursor;
GN   Name=BGLU25; OrderedLocusNames=Os06g0683300, LOC_Os06g46940;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17196101; DOI=10.1186/1471-2229-6-33;
RA   Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A.,
RA   Ketudat Cairns J.R.;
RT   "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12
RT   beta-glucosidase.";
RL   BMC Plant Biol. 6:33-33(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC         with release of beta-D-glucose.; EC=3.2.1.21;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q0DA21-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q0DA21-2; Sequence=VSP_038508, VSP_038509, VSP_038510;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF20302.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP008212; BAF20302.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014962; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK068614; BAG90993.1; -; mRNA.
DR   EMBL; AK120488; BAH00037.1; -; mRNA.
DR   RefSeq; XP_015643676.1; XM_015788190.1. [Q0DA21-1]
DR   AlphaFoldDB; Q0DA21; -.
DR   SMR; Q0DA21; -.
DR   STRING; 4530.OS06T0683300-01; -.
DR   CAZy; GH1; Glycoside Hydrolase Family 1.
DR   PaxDb; Q0DA21; -.
DR   PRIDE; Q0DA21; -.
DR   GeneID; 4341870; -.
DR   KEGG; osa:4341870; -.
DR   eggNOG; KOG0626; Eukaryota.
DR   InParanoid; Q0DA21; -.
DR   OrthoDB; 408001at2759; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   GO; GO:0033907; F:beta-D-fucosidase activity; IEA:UniProt.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProt.
DR   GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR   GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353; PTHR10353; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 1.
DR   PRINTS; PR00131; GLHYDRLASE1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW   Reference proteome; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..501
FT                   /note="Beta-glucosidase 25"
FT                   /id="PRO_0000390342"
FT   ACT_SITE        186
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        402
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         39
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         140
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         185
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         329
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         452
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         459..460
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        107
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        478
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        205..213
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         1..86
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:12869764"
FT                   /id="VSP_038508"
FT   VAR_SEQ         303..312
FT                   /note="GDRLPQFSTH -> ETIDCGSGNL (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:12869764"
FT                   /id="VSP_038509"
FT   VAR_SEQ         313..501
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:12869764"
FT                   /id="VSP_038510"
SQ   SEQUENCE   501 AA;  57191 MW;  09FEA57303269054 CRC64;
     MSLLTLVHIL VSFSACVEAI SRADFPPGFI FGTASSAYQY EGAVNEGQRG PTIWDTLTKR
     PGRVIDFSNA DVAVDHYHRY KEDVELMNDI GMDAYRFSIS WSRIFPNGTG EPNEEGLSYY
     NSLIDALLDK GIEPYVTLFH WDLPQALEDR YGGWLNSEII EDFVQYAFTC FKEFGDRVKH
     WITFNEPYNF AIDGYDLGIQ APGRCSILSH VFCREGKSST EPYIVAHNIL LAHAGAFRAY
     EQHFKNEQGG LIGIALNSRW YEPFSNADED TEAAARAMDF ELGWFLDPLM FGHYPPSMQK
     LAGDRLPQFS THASKLVSGS LDFVGINHYT TLYARNDRLR IRKLVMDDAS TDSAVIPTAY
     RHGKKIGETA ASSWLHIVPW GMFKLMKHVK EKYGNPPVVI TENGMDDANH PFSRLEDVLQ
     DDKRIQYHND YMSNLLDAIR KEGCNVHGYF VWSLLDNWEW NSGYTVRFGL YYIDYKNNLT
     RIPKASVQWF SQVLAQKTAI I