BGL27_ORYSJ
ID BGL27_ORYSJ Reviewed; 499 AA.
AC Q84YK7; A0A0P0XIN7; Q7EXZ5;
DT 15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Beta-glucosidase 27;
DE Short=Os8bglu27;
DE EC=3.2.1.21;
DE Flags: Precursor;
GN Name=BGLU27; OrderedLocusNames=Os08g0509200, LOC_Os08g39860;
GN ORFNames=B1168A08.29-1, B1168A08.29-2, OSJNBa0016N23.104-1,
GN OSJNBa0016N23.104-2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17196101; DOI=10.1186/1471-2229-6-33;
RA Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A.,
RA Ketudat Cairns J.R.;
RT "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12
RT beta-glucosidase.";
RL BMC Plant Biol. 6:33-33(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC with release of beta-D-glucose.; EC=3.2.1.21;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q84YK7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q84YK7-2; Sequence=VSP_038511;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
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DR EMBL; AP005816; BAD10670.1; -; Genomic_DNA.
DR EMBL; AP005816; BAD10671.1; -; Genomic_DNA.
DR EMBL; AP006049; BAC57391.1; -; Genomic_DNA.
DR EMBL; AP006049; BAD10730.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF24115.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT06181.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT06182.1; -; Genomic_DNA.
DR EMBL; AK067001; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK120430; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015650451.1; XM_015794965.1. [Q84YK7-1]
DR AlphaFoldDB; Q84YK7; -.
DR SMR; Q84YK7; -.
DR STRING; 4530.OS08T0509200-01; -.
DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR PaxDb; Q84YK7; -.
DR PRIDE; Q84YK7; -.
DR EnsemblPlants; Os08t0509200-01; Os08t0509200-01; Os08g0509200. [Q84YK7-1]
DR EnsemblPlants; Os08t0509200-02; Os08t0509200-02; Os08g0509200. [Q84YK7-2]
DR GeneID; 4345995; -.
DR Gramene; Os08t0509200-01; Os08t0509200-01; Os08g0509200. [Q84YK7-1]
DR Gramene; Os08t0509200-02; Os08t0509200-02; Os08g0509200. [Q84YK7-2]
DR KEGG; osa:4345995; -.
DR eggNOG; KOG0626; Eukaryota.
DR HOGENOM; CLU_001859_1_0_1; -.
DR InParanoid; Q84YK7; -.
DR OMA; YIDYERN; -.
DR OrthoDB; 408001at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; Q84YK7; OS.
DR GO; GO:0033907; F:beta-D-fucosidase activity; IEA:UniProt.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProt.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; PTHR10353; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..499
FT /note="Beta-glucosidase 27"
FT /id="PRO_0000390344"
FT ACT_SITE 192
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 407
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 43
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 146
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 191
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 335
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 456
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 463..464
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 111
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 361
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 408
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 414
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 415
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 211..219
FT /evidence="ECO:0000250"
FT VAR_SEQ 446..499
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_038511"
FT CONFLICT 401
FT /note="P -> T (in Ref. 4; AK067001)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 499 AA; 56804 MW; C49FA55790C20566 CRC64;
MRWLLLALFL VALVSNGAAV HGAFNRFSFP EDFIFGTGSA AYQYEGAVNE GGRGPSIWDT
YAHIPGKVED GSNGDVAVDF YHRYKEDLNF VTDMNMDAFR FSIAWSRILP NGTISGGINK
EGIAFYNSLI NEVISRGLKP FVTIFHFDTP QALEDKYRSF LSENIVKDFV DYADVCFREF
GDRVKSWNTF NEPMIFCAGG YGSGTKAPGR CSPYVSKKCA PGDSGNEPYV AGHNLLLAHA
EAVRLYRQKY QATQKGQIGI TQVSHWFVPY SDAAADKHAV RRSLDFMYGW FMDPIVFGDY
PGTMRKLVGD RLPKFTAEQS ELVKGSYDFI GLNYYTTNYA KSVLRRPSKL KPAYATDNWV
NQTAYRNGVP IGPPAFTKIF FTYAPGLREL LLYTKRKYND PDIYIAENGT DEANNSTIPI
AEALKDDNRI SFHYQHLRFT QLAIKEGVKV KGYFTWTFMD DFEWGDGYTG RFGLIYVDRE
TLKRYRKKSS YWFADFLKR
