BGL30_ORYSJ
ID BGL30_ORYSJ Reviewed; 500 AA.
AC Q0J0N4; A0A0P0XPL4; Q93ZK6;
DT 15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Beta-glucosidase 30;
DE Short=Os9bglu30;
DE EC=3.2.1.21;
DE Flags: Precursor;
GN Name=BGLU30; OrderedLocusNames=Os09g0491100, LOC_Os09g31430;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Orion;
RA Opassiri R., Ketudat Cairns J.R., Vichitphan S., Esen A.;
RT "Beta-glucosidase isozyme expression in rice.";
RL Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 135-500 (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17196101; DOI=10.1186/1471-2229-6-33;
RA Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A.,
RA Ketudat Cairns J.R.;
RT "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12
RT beta-glucosidase.";
RL BMC Plant Biol. 6:33-33(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC with release of beta-D-glucose.; EC=3.2.1.21;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0J0N4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0J0N4-2; Sequence=VSP_038512, VSP_038513;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
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DR EMBL; AY056828; AAL14713.1; -; mRNA.
DR EMBL; AP008215; BAF25453.1; -; Genomic_DNA.
DR EMBL; AP014965; BAT08727.1; -; Genomic_DNA.
DR EMBL; AK061340; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015610659.1; XM_015755173.1. [Q0J0N4-1]
DR RefSeq; XP_015610660.1; XM_015755174.1. [Q0J0N4-2]
DR AlphaFoldDB; Q0J0N4; -.
DR SMR; Q0J0N4; -.
DR STRING; 4530.OS09T0491100-01; -.
DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR PaxDb; Q0J0N4; -.
DR PRIDE; Q0J0N4; -.
DR EnsemblPlants; Os09t0491100-01; Os09t0491100-01; Os09g0491100. [Q0J0N4-1]
DR GeneID; 4347441; -.
DR Gramene; Os09t0491100-01; Os09t0491100-01; Os09g0491100. [Q0J0N4-1]
DR KEGG; osa:4347441; -.
DR eggNOG; KOG0626; Eukaryota.
DR InParanoid; Q0J0N4; -.
DR OMA; THQHSEN; -.
DR OrthoDB; 408001at2759; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR ExpressionAtlas; Q0J0N4; baseline and differential.
DR Genevisible; Q0J0N4; OS.
DR GO; GO:0033907; F:beta-D-fucosidase activity; IEA:UniProt.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProt.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; PTHR10353; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..500
FT /note="Beta-glucosidase 30"
FT /id="PRO_0000390347"
FT ACT_SITE 195
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 409
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 46
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 149
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 194
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 338
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 456
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 463..464
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 63
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 114
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 363
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 416
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 417
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 214..222
FT /evidence="ECO:0000250"
FT VAR_SEQ 377..436
FT /note="EFTPIFFNYPPGLRELLLYTKRRYNNPTIYVTENGIDEGNNSTLPEALKDGH
FT RIEFHSKH -> VSVSSSSTPRGDTTTRPSMLQKTASMRVTTAHCQRRSRMDTGSSSTQ
FT STCSSSTTPSRMG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_038512"
FT VAR_SEQ 437..500
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_038513"
FT CONFLICT 191
FT /note="T -> F (in Ref. 1; AAL14713)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 500 AA; 57388 MW; 1909568E1D9EFFD1 CRC64;
MGIRMGRRLL FTLFLGALFC NGVYAKFTRY SFPKDFIFGT GSAAYQYEGA YKEGGKGPSV
WDNFTHIPGK ILNNDNGDVA NDFYHRYKED VSLLKDMNMD AFRFSIAWTR ILPNGSLSGG
INKEGVAFYN SLINDVIAKG MIPFVTIFHW DTPLALESKY GGFLSEDIVK EYVDFAEVCF
REFGDRVKYW TTFNEPFTYS AYGYGKGVFA PGRCSSYVSK SCGVGDSSRE PYLVAHHIHL
SHAAAVQLYR TKYQPTQKGQ IGMVVVTHWF VPYDNSDADR GAVQRSLDFI YGWFMDPIVH
GDYPGTMRGW LGNRLPEFTP EQSAMVKGSY DFIGVNYYTT YYAKSIPPPN SNELSYDLDN
RANTTGFRNG KPIGPQEFTP IFFNYPPGLR ELLLYTKRRY NNPTIYVTEN GIDEGNNSTL
PEALKDGHRI EFHSKHLQFV NHAIKNGVNV KGYFTWTFMD CFEWGDGYLD RFGLIYVDRK
TLKRYRKESS YWIEDFLKRH
