BGL32_ORYSJ
ID BGL32_ORYSJ Reviewed; 508 AA.
AC Q0J0G2;
DT 15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 2.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Beta-glucosidase 32;
DE Short=Os9bglu32;
DE EC=3.2.1.21;
DE Flags: Precursor;
GN Name=BGLU32; OrderedLocusNames=Os09g0511700, LOC_Os09g33690;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17196101; DOI=10.1186/1471-2229-6-33;
RA Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A.,
RA Ketudat Cairns J.R.;
RT "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12
RT beta-glucosidase.";
RL BMC Plant Biol. 6:33-33(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC with release of beta-D-glucose.; EC=3.2.1.21;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0J0G2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0J0G2-2; Sequence=VSP_038514, VSP_038515;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP008215; BAF25553.1; -; Genomic_DNA.
DR EMBL; AP014965; BAT08914.1; -; Genomic_DNA.
DR EMBL; AK101420; BAG95059.1; -; mRNA.
DR RefSeq; XP_015612589.1; XM_015757103.1. [Q0J0G2-1]
DR AlphaFoldDB; Q0J0G2; -.
DR SMR; Q0J0G2; -.
DR STRING; 4530.OS09T0511700-01; -.
DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR PaxDb; Q0J0G2; -.
DR PRIDE; Q0J0G2; -.
DR EnsemblPlants; Os09t0511700-01; Os09t0511700-01; Os09g0511700. [Q0J0G2-2]
DR GeneID; 4347546; -.
DR Gramene; Os09t0511700-01; Os09t0511700-01; Os09g0511700. [Q0J0G2-2]
DR KEGG; osa:4347546; -.
DR eggNOG; KOG0626; Eukaryota.
DR InParanoid; Q0J0G2; -.
DR OMA; KYQVRPA; -.
DR OrthoDB; 408001at2759; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR GO; GO:0033907; F:beta-D-fucosidase activity; IEA:UniProt.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProt.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; PTHR10353; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..508
FT /note="Beta-glucosidase 32"
FT /id="PRO_0000390349"
FT ACT_SITE 196
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 404
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 50
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 150
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 195
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 339
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 451
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 458..459
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 222
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 441
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 215..223
FT /evidence="ECO:0000250"
FT VAR_SEQ 256..274
FT /note="AIQGGQIGITLLGWWYEPY -> LATGNSRRSDRDHSAGLVV (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_038514"
FT VAR_SEQ 275..508
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_038515"
SQ SEQUENCE 508 AA; 56799 MW; 52256C1319709A6C CRC64;
MAAGARALVP SPFIVVVFLL LAAAARDASA LTRHDFPEGF VFGAGTSAFQ VEGAAAEDGR
KPSIWDTFTH QGYSPGGAIA DVSADQYHHY KEDVKLMYDM GLDAYRFSIA WPRLIPDGRG
EINPKGLEYY NNLIDELIMH GIQPHVTIYH FDLPQALQDE YGGILSPRFI EDYTAYAEVC
FKNFGDRVKH WVTVNEPNIE PIGGYDAGVQ PPRRCSYPFG TNCTGGDSST EPYIVAHHLL
LAHASAVSIY RQKYQAIQGG QIGITLLGWW YEPYTDAVAD AAAAIRMNEF HIGWFMNPLV
HGDYPPVMRS RVGARLPSIT ASDSEKIRGS FDFIGINHYF VIFVQSSDAN HDQKLRDYYV
DAGVQENGGG GFDKEHYQLH PWALGKMLHH LKLKYGNPPV MIHENGDADS PETPGKIDYD
DDFRSDFLQS YLEVLHLSIR NGSNTRGYFV WSLLDGFEFL SGYGNRFGLC CVDFTAPART
RYVRSSARWY SDFLNGGELR PVKPFVAL
