SETA_ECOLI
ID SETA_ECOLI Reviewed; 392 AA.
AC P31675; P75639; Q6BF91;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Sugar efflux transporter A;
GN Name=setA; Synonyms=yabM; OrderedLocusNames=b0070, JW0069;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1630901; DOI=10.1093/nar/20.13.3305;
RA Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K.,
RA Mizobuchi K., Nakata A.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 0-
RT 2.4 min region.";
RL Nucleic Acids Res. 20:3305-3308(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP SEQUENCE REVISION TO 128.
RX PubMed=16397293; DOI=10.1093/nar/gkj405;
RA Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H.,
RA Thomson N.R., Wishart D., Wanner B.L.;
RT "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT -- 2005.";
RL Nucleic Acids Res. 34:1-9(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO 56.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP CHARACTERIZATION.
RX PubMed=10209755; DOI=10.1046/j.1365-2958.1999.01321.x;
RA Liu J.Y., Miller P.F., Gosink M., Olson E.R.;
RT "The identification of a new family of sugar efflux pumps in Escherichia
RT coli.";
RL Mol. Microbiol. 31:1845-1851(1999).
RN [6]
RP CHARACTERIZATION.
RX PubMed=10438463; DOI=10.1074/jbc.274.33.22977;
RA Liu J.Y., Miller P.F., Willard J., Olson E.R.;
RT "Functional and biochemical characterization of Escherichia coli sugar
RT efflux transporters.";
RL J. Biol. Chem. 274:22977-22984(1999).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Involved in the efflux of sugars. The physiological role may
CC be the detoxification of non-metabolizable sugar analogs. Can transport
CC IPTG, lactose and glucose. Has broad substrate specificity, with
CC preferences for glucosides or galactosides with alkyl or aryl
CC substituents.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Set
CC transporter family. {ECO:0000305}.
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DR EMBL; U00096; AAT48123.1; -; Genomic_DNA.
DR EMBL; AP009048; BAB96639.2; -; Genomic_DNA.
DR PIR; F64728; F64728.
DR RefSeq; WP_000637847.1; NZ_LN832404.1.
DR RefSeq; YP_025293.1; NC_000913.3.
DR AlphaFoldDB; P31675; -.
DR SMR; P31675; -.
DR STRING; 511145.b0070; -.
DR TCDB; 2.A.1.20.1; the major facilitator superfamily (mfs).
DR PaxDb; P31675; -.
DR PRIDE; P31675; -.
DR EnsemblBacteria; AAT48123; AAT48123; b0070.
DR EnsemblBacteria; BAB96639; BAB96639; BAB96639.
DR GeneID; 944793; -.
DR KEGG; ecj:JW0069; -.
DR KEGG; eco:b0070; -.
DR PATRIC; fig|1411691.4.peg.2211; -.
DR EchoBASE; EB1704; -.
DR eggNOG; COG2814; Bacteria.
DR HOGENOM; CLU_055598_3_0_6; -.
DR InParanoid; P31675; -.
DR OMA; GAQPFWV; -.
DR PhylomeDB; P31675; -.
DR BioCyc; EcoCyc:B0070-MON; -.
DR BioCyc; MetaCyc:B0070-MON; -.
DR PRO; PR:P31675; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0016020; C:membrane; IDA:EcoliWiki.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0005351; F:carbohydrate:proton symporter activity; IBA:GO_Central.
DR GO; GO:0034219; P:carbohydrate transmembrane transport; IDA:EcoCyc.
DR GO; GO:0036448; P:cellular response to glucose-phosphate stress; IMP:EcoCyc.
DR GO; GO:1904659; P:glucose transmembrane transport; IDA:EcoliWiki.
DR GO; GO:0015767; P:lactose transport; IDA:EcoliWiki.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004750; Sugar_efflux.
DR PANTHER; PTHR23535:SF2; PTHR23535:SF2; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00899; 2A0120; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..392
FT /note="Sugar efflux transporter A"
FT /id="PRO_0000209359"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..48
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..81
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..106
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..149
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 171
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..251
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273..282
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304..308
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 330..342
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..365
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 387..392
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 392 AA; 42713 MW; 8CBD368BBC448460 CRC64;
MIWIMTMARR MNGVYAAFML VAFMMGVAGA LQAPTLSLFL SREVGAQPFW IGLFYTVNAI
AGIGVSLWLA KRSDSQGDRR KLIIFCCLMA IGNALLFAFN RHYLTLITCG VLLASLANTA
MPQLFALARE YADNSAREVV MFSSVMRAQL SLAWVIGPPL AFMLALNYGF TVMFSIAAGI
FTLSLVLIAF MLPSVARVEL PSENALSMQG GWQDSNVRML FVASTLMWTC NTMYIIDMPL
WISSELGLPD KLAGFLMGTA AGLEIPAMIL AGYYVKRYGK RRMMVIAVAA GVLFYTGLIF
FNSRMALMTL QLFNAVFIGI VAGIGMLWFQ DLMPGRAGAA TTLFTNSIST GVILAGVIQG
AIAQSWGHFA VYWVIAVISV VALFLTAKVK DV
