BGL43_ARATH
ID BGL43_ARATH Reviewed; 501 AA.
AC Q9LV34; Q1PEP7;
DT 15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Beta-glucosidase 43;
DE Short=AtBGLU43;
DE EC=3.2.1.21;
DE Flags: Precursor;
GN Name=BGLU43; OrderedLocusNames=At3g18070; ORFNames=MRC8.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15604686; DOI=10.1007/s11103-004-0790-1;
RA Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R.,
RA Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.;
RT "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase
RT family 1.";
RL Plant Mol. Biol. 55:343-367(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC with release of beta-D-glucose.; EC=3.2.1.21;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9LV34-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9LV34-2; Sequence=VSP_038500;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02019.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB020749; BAB02019.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76042.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76043.1; -; Genomic_DNA.
DR EMBL; DQ446670; ABE65945.1; -; mRNA.
DR RefSeq; NP_001078176.1; NM_001084707.2. [Q9LV34-2]
DR RefSeq; NP_001325994.1; NM_001338322.1.
DR RefSeq; NP_188435.2; NM_112689.2. [Q9LV34-1]
DR AlphaFoldDB; Q9LV34; -.
DR SMR; Q9LV34; -.
DR STRING; 3702.AT3G18070.1; -.
DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR PaxDb; Q9LV34; -.
DR PRIDE; Q9LV34; -.
DR EnsemblPlants; AT3G18070.1; AT3G18070.1; AT3G18070. [Q9LV34-1]
DR EnsemblPlants; AT3G18070.2; AT3G18070.2; AT3G18070. [Q9LV34-2]
DR GeneID; 821332; -.
DR Gramene; AT3G18070.1; AT3G18070.1; AT3G18070. [Q9LV34-1]
DR Gramene; AT3G18070.2; AT3G18070.2; AT3G18070. [Q9LV34-2]
DR KEGG; ath:AT3G18070; -.
DR Araport; AT3G18070; -.
DR TAIR; locus:2092752; AT3G18070.
DR eggNOG; KOG0626; Eukaryota.
DR HOGENOM; CLU_001859_1_0_1; -.
DR InParanoid; Q9LV34; -.
DR OMA; YGDIKMY; -.
DR OrthoDB; 408001at2759; -.
DR PhylomeDB; Q9LV34; -.
DR BioCyc; ARA:AT3G18070-MON; -.
DR PRO; PR:Q9LV34; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LV34; baseline and differential.
DR Genevisible; Q9LV34; AT.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; PTHR10353; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Signal.
FT SIGNAL 1..14
FT /evidence="ECO:0000255"
FT CHAIN 15..501
FT /note="Beta-glucosidase 43"
FT /id="PRO_0000390316"
FT ACT_SITE 194
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 408
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 49
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 150
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 193
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 336
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 455
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 462..463
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 77
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 219
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 360
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 416
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 424
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 448
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 213..220
FT /evidence="ECO:0000250"
FT VAR_SEQ 92..168
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17147637"
FT /id="VSP_038500"
SQ SEQUENCE 501 AA; 57244 MW; 755B6F26B32B481E CRC64;
MFLFLLLLSA SRSGEESPSG DAVPLATGGL NRKSFPEGFL FGTATSAYQV EGETHQDGRG
PSIWDAFVKI PGKIANNATA EITVDQYHRY KEDVDLMQNL NIDAYRFSIS WSRIFPEGSG
KINSNGVAYY NRLIDYLIEK GITPYANLYH YDLPLALEQK YQGLLSKQGR FCGLRRVLFQ
TFGDRVKNWM TFNEPRVVAA LGYDNGIFAP GRCSEAFGNC TDGNSATEPY IVAHHLILAH
AAAVQRYRQN YQEKQKGRVG ILLDFVWFEP LTSSQADNDA AQRARDFHVG WFIHPIVYGE
YPNTLQNIVK ERLPKFTEEE VKMVKGSIDF VGINQYTTYF MSDPKISTTP KDLGYQQDWN
VTFNFAKNGT PIGPRAHSEW LYNVPWGMYK ALMYIEERYG NPTMILSENG MDDPGNITLT
QGLNDTTRVK YYRDYLVQLK KAVDDGANLT GYFAWSLLDN FEWLSGYTSR FGIVYVDYKD
LKRYPKMSAL WFKQLLKRDQ K
