SEY1_ASPOR
ID SEY1_ASPOR Reviewed; 860 AA.
AC Q2UNK6;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 2.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Protein sey1 {ECO:0000255|HAMAP-Rule:MF_03109};
DE EC=3.6.5.- {ECO:0000255|HAMAP-Rule:MF_03109};
GN Name=sey1; ORFNames=AO090001000326;
OS Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=510516;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 42149 / RIB 40;
RX PubMed=16372010; DOI=10.1038/nature04300;
RA Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA Kikuchi H.;
RT "Genome sequencing and analysis of Aspergillus oryzae.";
RL Nature 438:1157-1161(2005).
CC -!- FUNCTION: Cooperates with the reticulon proteins and tubule-shaping DP1
CC family proteins to generate and maintain the structure of the tubular
CC endoplasmic reticulum network. Has GTPase activity, which is required
CC for its function in ER organization. {ECO:0000255|HAMAP-Rule:MF_03109}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000255|HAMAP-Rule:MF_03109}; Multi-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_03109}. Note=Enriched in the cortical ER.
CC Concentrated in punctae along the ER tubules. {ECO:0000255|HAMAP-
CC Rule:MF_03109}.
CC -!- SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase
CC superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU01052}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE56859.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP007154; BAE56859.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; Q2UNK6; -.
DR SMR; Q2UNK6; -.
DR STRING; 510516.Q2UNK6; -.
DR Proteomes; UP000006564; Chromosome 2.
DR GO; GO:0032541; C:cortical endoplasmic reticulum; IEA:EnsemblFungi.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0048309; P:endoplasmic reticulum inheritance; IEA:EnsemblFungi.
DR GO; GO:0016320; P:endoplasmic reticulum membrane fusion; IEA:EnsemblFungi.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_03109; Sey1; 1.
DR InterPro; IPR030386; G_GB1_RHD3_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR008803; RHD3/Sey1.
DR PANTHER; PTHR45923; PTHR45923; 1.
DR Pfam; PF05879; RHD3; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51715; G_GB1_RHD3; 1.
PE 3: Inferred from homology;
KW Coiled coil; Endoplasmic reticulum; GTP-binding; Hydrolase; Membrane;
KW Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..860
FT /note="Protein sey1"
FT /id="PRO_0000384973"
FT TOPO_DOM 1..742
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03109"
FT TRANSMEM 743..763
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03109"
FT TOPO_DOM 764..766
FT /note="Lumenal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03109"
FT TRANSMEM 767..787
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03109"
FT TOPO_DOM 788..860
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03109"
FT DOMAIN 51..300
FT /note="GB1/RHD3-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01052"
FT REGION 818..860
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 443..501
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03109"
FT BINDING 61..68
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03109"
SQ SEQUENCE 860 AA; 96683 MW; BCBBAC895CC7D234 CRC64;
MATNGHFASI GNSASDTTAY EHGVQVIDEN KEFKNPNLSQ YLSLENVTPS GFNYHLISVF
GSQSTGKSTL LNHLFGTHFS VMSELERRQT TKGIWMSKNK NESSSMASNI LVMDVEGTDG
RERGEDQDFE RKSALFALAT SEVLIVNIWE HQVGLYQGAN MGLLKTVFEV NLQLFLKDKN
TTHRSLLFFV IRDYSGMTPL QNLQKTLMED MARLWDSISK PGGLENSNVH DYFDFQFYGL
PHKGYQPEKF VEETQKLSLR FCDGQRDPNL DARKGEFSDG GVFLPEYHRR IPADGFSRYA
EGIWDQIVNN KDLDLPTQQE LLAQFRCDEI LREVMVAFDE TIVPFEDKQS QAARLGEPEI
LGGLGAAMRS SRTKAVKAFE SEASRYHKGV YQRKRAELES KADTRLKTLF QGQLNAAHKS
GISEFSEAVT AAVKSGQKKG TGYDFAEIVN EEAKKAVDKF EEVARATVVD GTSWSDYKQE
LALYEKELAE VSARLRRDEM RRLASRVERW VQSRLGESVG LEFNALGSGR AGGGAPEKGD
QPTEKKFWDR VWNVFVETVL DAERRFTDRA SSFDASLEEV DVGLWRLRRK SWGVLRAKID
EEMIEGNLLL KLRENFEDKF RYDDAGVPRI WRPTDDIEGI YTRARESTLT LIPLLSKFRL
DETSAPPPLD RWIGHTPSSA TSADEEDLAP IGGVDEEEGK SLEEEMTIVS DAKRQELTVR
FKKAADGVYV EAKRSAIGGM TQVPLYFYGL LLALGWNEII AVLRNPAYFF LLFVCAVGAY
ITYQLNLWGP IIKMTEAASN QAVTEGKKRL REFLESSDTG RQAIAMSTPG GSGRGGEEHE
MSRLNQQGKS AAADEDVDDL