SF33K_ADE40
ID SF33K_ADE40 Reviewed; 211 AA.
AC P11805;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Protein 33K {ECO:0000250|UniProtKB:P24939};
DE Short=L4-33K;
DE AltName: Full=Splicing factor 33K {ECO:0000305};
DE AltName: Full=Terminase, small subunit {ECO:0000250|UniProtKB:P24940};
GN ORFNames=L4;
OS Human adenovirus F serotype 40 (HAdV-40) (Human adenovirus 40).
OC Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC Rowavirales; Adenoviridae; Mastadenovirus; Human mastadenovirus F.
OX NCBI_TaxID=28284;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Dugan;
RX PubMed=8263936; DOI=10.1006/jmbi.1993.1687;
RA Davison A.J., Telford E.A., Watson M.S., McBride K., Mautner V.;
RT "The DNA sequence of adenovirus type 40.";
RL J. Mol. Biol. 234:1308-1316(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 176-211.
RX PubMed=3279700; DOI=10.1016/0042-6822(88)90227-9;
RA Vos H.L., der Lee F.M., Reemst A.M.C.B., van Loon A.E., Sussenbach J.S.;
RT "The genes encoding the DNA binding protein and the 23K protease of
RT adenovirus types 40 and 41.";
RL Virology 163:1-10(1988).
CC -!- FUNCTION: Promotes alternative splicing of late transcripts by
CC promoting splicing at weak 3' splice sites. Required for the temporal
CC activation of major late pre-mRNA splicing at late times of infection.
CC Induces the splicing and expression of the late capsid vertex protein
CC (By similarity). {ECO:0000250}.
CC -!- FUNCTION: Probably functions as the small terminase that is part of the
CC molecular motor that translocates genomic DNA in empty capsid during
CC DNA packaging. This motor is located at a unique vertex and comprises
CC at least the IVa2 ATPase, the small terminase 33K and probably a
CC portal. Forms a ring-like structure of about 17 nm in which genomic DNA
CC is translocated into the capsid. Stimulates IVa2 ATPase activity in the
CC presence of the viral genome. Once the DNA is packaged, the terminase
CC detaches: the 33K protein is present in the empty particles, but not in
CC the mature virions. Also involved in virion assembly.
CC {ECO:0000250|UniProtKB:P24940}.
CC -!- SUBUNIT: Homooligomer. Interacts with DBP; this interaction occurs at a
CC unique vertex during genome packaging. Interacts with IVa2; this
CC interaction occurs at a unique vertex during genome packaging and seems
CC to potentiate IVa2 and 33K oligomerization.
CC {ECO:0000250|UniProtKB:P24940}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250|UniProtKB:P24940}.
CC Note=At late time of infection, reorganized from the nuclear margin to
CC ring-like structures at viral replication centers.
CC {ECO:0000250|UniProtKB:P24940}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Protein 33K; Synonyms=Splicing factor 33K, L4-33K;
CC IsoId=P11805-1; Sequence=Displayed;
CC Name=Packaging protein 2; Synonyms=Packaging protein 22K, L4-22K;
CC IsoId=P11805-2; Sequence=Not described;
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC -!- DOMAIN: The tiny Arg-Ser repeat region (RS repeat) is necessary for the
CC splicing enhancer function. {ECO:0000250}.
CC -!- PTM: Phosphorylated in vitro by human PKA and PRKDC. PRKDC inhibits,
CC whereas PKA activates the splicing factor (By similarity).
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: All late proteins expressed from the major late promoter
CC are produced by alternative splicing and alternative polyadenylation of
CC the same gene giving rise to non-overlapping ORFs. Expression of
CC packaging protein 2 and splicing factor is controlled by a L4 promoter
CC distinct from the major late promoter (By similarity). {ECO:0000250}.
CC -!- MISCELLANEOUS: [Isoform Protein 33K]: Spliced isoform.
CC -!- MISCELLANEOUS: [Isoform Packaging protein 2]: Unspliced isoform.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the adenoviridae splicing factor family.
CC {ECO:0000305}.
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DR EMBL; L19443; AAC13956.1; -; Genomic_DNA.
DR EMBL; M19316; AAA52199.1; -; Genomic_DNA.
DR RefSeq; NP_040866.1; NC_001454.1.
DR DNASU; 2715920; -.
DR GeneID; 2715920; -.
DR Proteomes; UP000151954; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0019073; P:viral DNA genome packaging; IEA:InterPro.
DR InterPro; IPR021304; Adeno_L4-33K/L4-22K.
DR Pfam; PF11081; DUF2890; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Host nucleus; Host-virus interaction; Late protein;
KW mRNA processing; Phosphoprotein; Reference proteome; Viral capsid assembly;
KW Viral genome packaging; Viral release from host cell.
FT CHAIN 1..211
FT /note="Protein 33K"
FT /id="PRO_0000221913"
FT REGION 1..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 107..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 154..181
FT /note="Necessary for nuclear subcellular location"
FT /evidence="ECO:0000250"
FT REGION 160..180
FT /note="RS-repeat; required for splicing enhancer activity"
FT /evidence="ECO:0000250"
FT COMPBIAS 28..63
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 211 AA; 24162 MW; AAD90FC8B34EB3CA CRC64;
MPPKGNKHPI AQRQSQQKLQ KQWDEEETWD DSQAEEVSDE EAEEQMESWD SLDEEDLEDV
EEETIASDKA PSFKKPVRSQ PPKTIPPLPP QPCSLKASRR WDTVSIAGSP TAPAAPTKRL
EKTPRVRKTS SAIATRQDSP ATQELRKRIF PTLYAIFQQS RGQQLELKVK NRSLRSLTRS
CLYHRSEDQL QRTLEDAEAL FNKYCSVSLK D
