SF33K_ADE41
ID SF33K_ADE41 Reviewed; 217 AA.
AC P19416; Q64818;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 02-JUN-2021, entry version 53.
DE RecName: Full=Protein 33K {ECO:0000250|UniProtKB:P24939};
DE Short=L4-33K;
DE AltName: Full=Splicing factor 33K {ECO:0000305};
DE AltName: Full=Terminase, small subunit {ECO:0000250|UniProtKB:P24940};
GN ORFNames=L4;
OS Human adenovirus F serotype 41 (HAdV-41) (Human adenovirus 41).
OC Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC Rowavirales; Adenoviridae; Mastadenovirus; Human mastadenovirus F.
OX NCBI_TaxID=10524;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Tak;
RX PubMed=2349115; DOI=10.1093/nar/18.10.3069;
RA Slemenda S.B., Pieniazek N.J., Velarde J. Jr., Pieniazek D., Luftig R.B.;
RT "Nucleotide sequence of the region coding for 100K and 33K proteins of
RT human enteric adenovirus type 41 (Tak).";
RL Nucleic Acids Res. 18:3069-3069(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 203-217.
RC STRAIN=Tak;
RX PubMed=2762136; DOI=10.1093/nar/17.13.5398;
RA Pieniazek N.J., Velarde J. Jr., Pieniazek D., Luftig R.B.;
RT "Nucleotide sequence of human enteric adenovirus type 41 hexon-associated
RT protein VIII precursor (pVIII) including the early region E3 promoter.";
RL Nucleic Acids Res. 17:5398-5398(1989).
CC -!- FUNCTION: Promotes alternative splicing of late transcripts by
CC promoting splicing at weak 3' splice sites. Required for the temporal
CC activation of major late pre-mRNA splicing at late times of infection.
CC Induces the splicing and expression of the late capsid vertex protein
CC (By similarity). {ECO:0000250}.
CC -!- FUNCTION: Probably functions as the small terminase that is part of the
CC molecular motor that translocates genomic DNA in empty capsid during
CC DNA packaging. This motor is located at a unique vertex and comprises
CC at least the IVa2 ATPase, the small terminase 33K and probably a
CC portal. Forms a ring-like structure of about 17 nm in which genomic DNA
CC is translocated into the capsid. Stimulates IVa2 ATPase activity in the
CC presence of the viral genome. Once the DNA is packaged, the terminase
CC detaches: the 33K protein is present in the empty particles, but not in
CC the mature virions. Also involved in virion assembly.
CC {ECO:0000250|UniProtKB:P24940}.
CC -!- SUBUNIT: Homooligomer. Interacts with DBP; this interaction occurs at a
CC unique vertex during genome packaging. Interacts with IVa2; this
CC interaction occurs at a unique vertex during genome packaging and seems
CC to potentiate IVa2 and 33K oligomerization.
CC {ECO:0000250|UniProtKB:P24940}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250|UniProtKB:P24940}.
CC Note=At late time of infection, reorganized from the nuclear margin to
CC ring-like structures at viral replication centers.
CC {ECO:0000250|UniProtKB:P24940}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Protein 33K; Synonyms=Splicing factor 33K, L4-33K;
CC IsoId=P19416-1; Sequence=Displayed;
CC Name=Packaging protein 2; Synonyms=Packaging protein 22K, L4-22K;
CC IsoId=P19416-2; Sequence=Not described;
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC -!- DOMAIN: The tiny Arg-Ser repeat region (RS repeat) is necessary for the
CC splicing enhancer function. {ECO:0000250}.
CC -!- PTM: Phosphorylated in vitro by human PKA and PRKDC. PRKDC inhibits,
CC whereas PKA activates the splicing factor (By similarity).
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: All late proteins expressed from the major late promoter
CC are produced by alternative splicing and alternative polyadenylation of
CC the same gene giving rise to non-overlapping ORFs. Expression of
CC packaging protein 2 and splicing factor is controlled by a L4 promoter
CC distinct from the major late promoter (By similarity). {ECO:0000250}.
CC -!- MISCELLANEOUS: [Isoform Protein 33K]: Spliced isoform.
CC -!- MISCELLANEOUS: [Isoform Packaging protein 2]: Unspliced isoform.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the adenoviridae splicing factor family.
CC {ECO:0000305}.
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DR EMBL; X52532; CAB38632.1; -; Genomic_DNA.
DR EMBL; X15137; CAA33236.1; -; Genomic_DNA.
DR PIR; S10212; S10212.
DR PRIDE; P19416; -.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0019073; P:viral DNA genome packaging; IEA:InterPro.
DR InterPro; IPR021304; Adeno_L4-33K/L4-22K.
DR Pfam; PF11081; DUF2890; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Host nucleus; Host-virus interaction; Late protein;
KW mRNA processing; Phosphoprotein; Viral capsid assembly;
KW Viral genome packaging; Viral release from host cell.
FT CHAIN 1..217
FT /note="Protein 33K"
FT /id="PRO_0000221914"
FT REGION 1..142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 160..187
FT /note="Necessary for nuclear subcellular location"
FT /evidence="ECO:0000250"
FT REGION 166..186
FT /note="RS-repeat; required for splicing enhancer activity"
FT /evidence="ECO:0000250"
FT COMPBIAS 10..25
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..62
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..139
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 217 AA; 24741 MW; B6ECDA6B3C24EC3E CRC64;
MPPKGNKQAI ADRRSQKQQK LQEQWDEEEE SWDDSQAEEV SDEEEMESWE SLDEELEDKP
PKDEEEEIIA SAAAPSSKEP ARSQPPTGKV GPSPPRPGLL KASRRWDTVS IAGSPPAPVA
PTKRSEKTTR PRKEKTSAIA TRQDTPVAQE LRKRIFPTLY AIFQQSRGQQ LELKVKNRSL
RSLTRSCLYH RREDQLQRTL EDAEALFNKY CSVSLKD
