SF3A1_ARATH
ID SF3A1_ARATH Reviewed; 785 AA.
AC Q8RXF1; Q0WWB5; Q9MA20;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 2.
DT 25-MAY-2022, entry version 145.
DE RecName: Full=Probable splicing factor 3A subunit 1;
GN OrderedLocusNames=At1g14650; ORFNames=T5E21.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [6]
RP STRUCTURE BY NMR OF 683-780.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of ubiquitin-like domain in splicing factor AAL91182.";
RL Submitted (NOV-2004) to the PDB data bank.
CC -!- SUBUNIT: Component of splicing factor SF3A which is composed of three
CC subunits. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: SURP motif 2 mediates direct binding to SF3A3. {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF63169.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC010657; AAF63169.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29196.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29197.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM61051.1; -; Genomic_DNA.
DR EMBL; AY081293; AAL91182.1; -; mRNA.
DR EMBL; AK226440; BAE98583.1; -; mRNA.
DR PIR; G86280; G86280.
DR RefSeq; NP_001117289.1; NM_001123817.1.
DR RefSeq; NP_001319005.1; NM_001332131.1.
DR RefSeq; NP_172917.1; NM_101332.4.
DR PDB; 1WE6; NMR; -; A=683-780.
DR PDBsum; 1WE6; -.
DR AlphaFoldDB; Q8RXF1; -.
DR SMR; Q8RXF1; -.
DR BioGRID; 23267; 3.
DR IntAct; Q8RXF1; 1.
DR MINT; Q8RXF1; -.
DR STRING; 3702.AT1G14650.2; -.
DR iPTMnet; Q8RXF1; -.
DR PaxDb; Q8RXF1; -.
DR PRIDE; Q8RXF1; -.
DR ProteomicsDB; 234556; -.
DR EnsemblPlants; AT1G14650.1; AT1G14650.1; AT1G14650.
DR EnsemblPlants; AT1G14650.2; AT1G14650.2; AT1G14650.
DR EnsemblPlants; AT1G14650.3; AT1G14650.3; AT1G14650.
DR GeneID; 838027; -.
DR Gramene; AT1G14650.1; AT1G14650.1; AT1G14650.
DR Gramene; AT1G14650.2; AT1G14650.2; AT1G14650.
DR Gramene; AT1G14650.3; AT1G14650.3; AT1G14650.
DR KEGG; ath:AT1G14650; -.
DR Araport; AT1G14650; -.
DR TAIR; locus:2204528; AT1G14650.
DR eggNOG; KOG0007; Eukaryota.
DR HOGENOM; CLU_013259_1_0_1; -.
DR InParanoid; Q8RXF1; -.
DR OMA; MGEDQND; -.
DR OrthoDB; 1256232at2759; -.
DR PhylomeDB; Q8RXF1; -.
DR EvolutionaryTrace; Q8RXF1; -.
DR PRO; PR:Q8RXF1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8RXF1; baseline and differential.
DR Genevisible; Q8RXF1; AT.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005686; C:U2 snRNP; IBA:GO_Central.
DR GO; GO:0071004; C:U2-type prespliceosome; IBA:GO_Central.
DR GO; GO:0005684; C:U2-type spliceosomal complex; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:InterPro.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR CDD; cd01800; Ubl_SF3a120; 1.
DR Gene3D; 1.10.10.790; -; 2.
DR InterPro; IPR045146; SF3A1.
DR InterPro; IPR022030; SF3A1_dom.
DR InterPro; IPR035563; SF3As1_ubi.
DR InterPro; IPR000061; Surp.
DR InterPro; IPR035967; SWAP/Surp_sf.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR15316; PTHR15316; 1.
DR Pfam; PF12230; PRP21_like_P; 1.
DR Pfam; PF01805; Surp; 2.
DR Pfam; PF00240; ubiquitin; 1.
DR SMART; SM00648; SWAP; 2.
DR SMART; SM00213; UBQ; 1.
DR SUPFAM; SSF109905; SSF109905; 2.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50128; SURP; 2.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Repeat; Spliceosome.
FT CHAIN 1..785
FT /note="Probable splicing factor 3A subunit 1"
FT /id="PRO_0000114919"
FT REPEAT 71..113
FT /note="SURP motif 1"
FT REPEAT 193..235
FT /note="SURP motif 2"
FT DOMAIN 707..782
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 124..175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 522..554
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 639..713
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..144
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 540..554
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 649..672
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 673..688
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 196
FT /note="Critical for binding to SF3A3"
FT /evidence="ECO:0000250"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CONFLICT 289
FT /note="D -> G (in Ref. 3; AAL91182)"
FT /evidence="ECO:0000305"
FT HELIX 686..688
FT /evidence="ECO:0007829|PDB:1WE6"
FT HELIX 692..698
FT /evidence="ECO:0007829|PDB:1WE6"
FT STRAND 703..707
FT /evidence="ECO:0007829|PDB:1WE6"
FT STRAND 713..715
FT /evidence="ECO:0007829|PDB:1WE6"
FT STRAND 718..723
FT /evidence="ECO:0007829|PDB:1WE6"
FT STRAND 725..728
FT /evidence="ECO:0007829|PDB:1WE6"
FT HELIX 729..739
FT /evidence="ECO:0007829|PDB:1WE6"
FT TURN 744..746
FT /evidence="ECO:0007829|PDB:1WE6"
FT STRAND 747..750
FT /evidence="ECO:0007829|PDB:1WE6"
FT STRAND 752..755
FT /evidence="ECO:0007829|PDB:1WE6"
FT TURN 762..766
FT /evidence="ECO:0007829|PDB:1WE6"
FT STRAND 768..770
FT /evidence="ECO:0007829|PDB:1WE6"
FT STRAND 772..776
FT /evidence="ECO:0007829|PDB:1WE6"
SQ SEQUENCE 785 AA; 87594 MW; 917E388B77472F8D CRC64;
MFSSMQILPL EAPPTDGKLG PLPPSQLTDQ EVEERELQAE QNNSNLAPPA AVATHTRTIG
IIHPPPDIRT IVEKTAQFVS KNGLEFEKRI IVSNEKNAKF NFLKSSDPYH AFYQHKLTEY
RAQNKDGAQG TDDSDGTTDP QLDTGAADES EAGDTQPDLQ AQFRIPSKPL EAPEPEKYTV
RLPEGITGEE LDIIKLTAQF VARNGKSFLT GLSNRENNNP QFHFMKPTHS MFTFFTSLVD
AYSEVLMPPK DLKEKLRKSA ADLTTVLERC LHRLEWDRSQ EQQKKKEEDE KELERVQMAM
IDWHDFVVVE SIDFADEEDE ELPPPMTLDE VIRRSKASAM EEDEIVEPGK EVEMEMDEEE
VKLVAEGMRA ANLEENVKIE NVHDEEAPMR IVKNWKRPED RIPTERDPTK VVISPITGEL
IPINEMSEHM RISLIDPKFK EQKDRMFAKI RETTLAQDDE IAKNIVGLAR LRPDIFGTTE
EEVSNAVKAE IEKKKDEQPK QVIWDGHTGS IGRTANQALS QNANGEEQGD GVYGDPNSFP
GPAALPPPRP GVPIVRPLPP PPNLALNLPR PPPSAQYPGA PRPLGVPMMQ PMHQQHQLTM
PGPPGHPQMM MNRPPQMQPG MHVPPPPGSQ FAHHMQIPRP YGQLPPSAMG MMQPPPMPGM
APPPPPEEAP PPLPEEPEAK RQKFDESALV PEDQFLAQHP GPATIRVSKP NENDGQFMEI
TVQSLSENVG SLKEKIAGEI QIPANKQKLS GKAGFLKDNM SLAHYNVGAG EILTLSLRER
GGRKR
