SF3A1_DICDI
ID SF3A1_DICDI Reviewed; 760 AA.
AC Q86A14; Q1ZXM9;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Probable splicing factor 3A subunit 1;
GN Name=sf3a1; ORFNames=DDB_G0272676;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Involved in pre-mRNA splicing as a component of the splicing
CC factor SF3A complex that contributes to the assembly of the 17S U2
CC snRNP, and the subsequent assembly of the spliceosome.
CC {ECO:0000250|UniProtKB:Q15459}.
CC -!- SUBUNIT: Component of splicing factor SF3A which associates with the
CC splicing factor SF3B and a 12S RNA unit to form the mature 17S U2 small
CC nuclear ribonucleoprotein complex (17S U2 snRNP). Identified in the
CC spliceosome 'E' complex, a precursor of the spliceosome 'A' complex.
CC Identified in the spliceosome 'A' and 'B' complexes. Identified in the
CC spliceosome 'C' complex. {ECO:0000250|UniProtKB:Q15459}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15459}.
CC -!- SIMILARITY: Belongs to the SF3A1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAFI02000008; EAS66956.1; -; Genomic_DNA.
DR RefSeq; XP_001134622.1; XM_001134622.1.
DR AlphaFoldDB; Q86A14; -.
DR SMR; Q86A14; -.
DR STRING; 44689.DDB0233180; -.
DR PaxDb; Q86A14; -.
DR PRIDE; Q86A14; -.
DR EnsemblProtists; EAS66956; EAS66956; DDB_G0272676.
DR GeneID; 8618597; -.
DR KEGG; ddi:DDB_G0272676; -.
DR dictyBase; DDB_G0272676; sf3a1.
DR eggNOG; KOG0007; Eukaryota.
DR HOGENOM; CLU_013259_1_0_1; -.
DR InParanoid; Q86A14; -.
DR OMA; HFSARMP; -.
DR PhylomeDB; Q86A14; -.
DR Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR PRO; PR:Q86A14; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005681; C:spliceosomal complex; ISS:dictyBase.
DR GO; GO:0005686; C:U2 snRNP; IBA:GO_Central.
DR GO; GO:0071004; C:U2-type prespliceosome; IBA:GO_Central.
DR GO; GO:0005684; C:U2-type spliceosomal complex; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:InterPro.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR CDD; cd01800; Ubl_SF3a120; 1.
DR Gene3D; 1.10.10.790; -; 2.
DR InterPro; IPR045146; SF3A1.
DR InterPro; IPR022030; SF3A1_dom.
DR InterPro; IPR035563; SF3As1_ubi.
DR InterPro; IPR000061; Surp.
DR InterPro; IPR035967; SWAP/Surp_sf.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR15316; PTHR15316; 1.
DR Pfam; PF12230; PRP21_like_P; 1.
DR Pfam; PF01805; Surp; 2.
DR Pfam; PF00240; ubiquitin; 1.
DR SMART; SM00648; SWAP; 2.
DR SMART; SM00213; UBQ; 1.
DR SUPFAM; SSF109905; SSF109905; 2.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50128; SURP; 2.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 3: Inferred from homology;
KW Coiled coil; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Repeat; Spliceosome.
FT CHAIN 1..760
FT /note="Probable splicing factor 3A subunit 1"
FT /id="PRO_0000327906"
FT REPEAT 16..58
FT /note="SURP motif 1"
FT REPEAT 183..225
FT /note="SURP motif 2"
FT DOMAIN 680..757
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT REGION 77..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 309..348
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 360..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 442..464
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 487..509
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 544..583
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 624..657
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 655..677
FT /note="Required and sufficient for nuclear import"
FT /evidence="ECO:0000250|UniProtKB:Q15459"
FT COILED 259..290
FT /evidence="ECO:0000255"
FT COMPBIAS 81..144
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..162
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 371..388
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 446..464
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 544..560
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 632..653
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 760 AA; 84276 MW; 03C5ED04C5D17137 CRC64;
MANEIITPTE GELKTIIDKT AAYAAKLGES FENKVKQREG HNAKFNFMKE GDQYYPYYRN
KIVENKAKIQ ADAAAAAAAA NPKTPSTTTT TTTTTTATTP LPTATATTTS PTPTTSSTIV
PVPQTNSTQQ LQQQQQQQQQ TTPVFEKPQP PPPPPPKKEP TQPDPLLYIL DVPDFMTPLE
LDTIRLTAQF IAKNGDSFFM ELASREVKNS QFDFLKPTNH LYEWFRALVE SYAQIIYPPQ
GIKEQLKSNY FSNKQTILER AMNRCEYNQL KEIEEQKKEE REDEEKTIIA SIDWHDFVIV
DTIEFNEDDL DDLPQPRTFD QLIAGDTPFG GSDFDNDGHG SNGKGGQDME MEMDMEMEMD
DEDNNNNEDE IEESSLSSTN TTGNLPPKDQ SKLKIVKDYQ KSNSSVKSNA PTKLTQLCQF
CKQEIPLDEM QEHMRIELIQ KQQRDSRLGG GGSSNNNNNL TNTLTQDDDI ARNLQSFASK
RVDIFGETES SKKQDEQPTQ APKVIWDGHS GSIPRVQAAQ QAAQLAAQQA AQQKAAAIAA
QQASQQQASQ QQQQQLQPPQ PIGIHHHPQR HLPPGMMPPG MMPPGMMPPG MMPPGMVPPP
PFGMIPPGMV PPPPPGLMIP TAPPGLMIPP TQQQQLPPQT NTSPSSSSSI KVEEPQSKKL
KIDDVLIPES VWLQNNPNPV NLTVEMADKS NIYQITLQPT DSISLLKEKI KELNGMPTNK
QKLQAPGLSI LKDTCSIAFY NLKSLAIVTC GQKKKGGKKK
