SF3B1_XENLA
ID SF3B1_XENLA Reviewed; 1307 AA.
AC O57683;
DT 29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Splicing factor 3B subunit 1;
DE AltName: Full=146 kDa nuclear protein;
DE AltName: Full=Pre-mRNA-splicing factor SF3b 155 kDa subunit;
DE Short=SF3b155;
DE AltName: Full=Spliceosome-associated protein 155;
DE Short=SAP 155;
GN Name=sf3b1;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9436997; DOI=10.1091/mbc.9.1.143;
RA Schmidt-Zachmann M.S., Knecht S., Kraemer A.;
RT "Molecular characterization of a novel, widespread nuclear protein that
RT colocalizes with spliceosome components.";
RL Mol. Biol. Cell 9:143-160(1998).
CC -!- FUNCTION: Involved in pre-mRNA splicing as a component of the splicing
CC factor SF3B complex. SF3B complex is required for 'A' complex assembly
CC formed by the stable binding of U2 snRNP to the branchpoint sequence
CC (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex
CC upstream of the branch site is essential, it may anchor U2 snRNP to the
CC pre-mRNA. May also be involved in the assembly of the 'E' complex.
CC Belongs also to the minor U12-dependent spliceosome, which is involved
CC in the splicing of rare class of nuclear pre-mRNA intron.
CC {ECO:0000250|UniProtKB:O75533}.
CC -!- SUBUNIT: Component of splicing factor SF3B complex which is composed of
CC at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6,
CC PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a
CC 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex
CC (U2 snRNP). {ECO:0000250|UniProtKB:O75533}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O75533}.
CC -!- SIMILARITY: Belongs to the SF3B1 family. {ECO:0000305}.
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DR EMBL; Y08997; CAA70201.1; -; mRNA.
DR PIR; T30887; T30887.
DR RefSeq; NP_001084150.1; NM_001090681.1.
DR AlphaFoldDB; O57683; -.
DR BMRB; O57683; -.
DR SMR; O57683; -.
DR PRIDE; O57683; -.
DR DNASU; 399336; -.
DR GeneID; 399336; -.
DR KEGG; xla:399336; -.
DR CTD; 399336; -.
DR Xenbase; XB-GENE-959871; sf3b1.S.
DR OMA; WMRICHE; -.
DR OrthoDB; 245208at2759; -.
DR Proteomes; UP000186698; Chromosome 9_10S.
DR Bgee; 399336; Expressed in egg cell and 19 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; IEA:InterPro.
DR GO; GO:0000245; P:spliceosomal complex assembly; IEA:InterPro.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR015016; SF3b_su1.
DR InterPro; IPR038737; SF3b_su1-like.
DR PANTHER; PTHR12097; PTHR12097; 2.
DR Pfam; PF08920; SF3b1; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 2: Evidence at transcript level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat;
KW Spliceosome.
FT CHAIN 1..1307
FT /note="Splicing factor 3B subunit 1"
FT /id="PRO_0000174325"
FT REPEAT 532..571
FT /note="HEAT 1"
FT REPEAT 572..606
FT /note="HEAT 2"
FT REPEAT 607..644
FT /note="HEAT 3"
FT REPEAT 646..680
FT /note="HEAT 4"
FT REPEAT 683..721
FT /note="HEAT 5"
FT REPEAT 766..804
FT /note="HEAT 6"
FT REPEAT 846..884
FT /note="HEAT 7"
FT REPEAT 1013..1051
FT /note="HEAT 8"
FT REPEAT 1055..1093
FT /note="HEAT 9"
FT REPEAT 1125..1163
FT /note="HEAT 10"
FT REPEAT 1166..1204
FT /note="HEAT 11"
FT REGION 61..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 111..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 169..363
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..129
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 205..232
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 345..359
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1307 AA; 146215 MW; 6B226D5F036C69B3 CRC64;
MAKVAKTHED IEAQIREIQG KKAALDEEEG VGLDSTGYFD QEIYGGSDSR FTGYVTSIAA
NEQEDDDDDV SSATFEQKKP GYHAPVALLN DIPQSTEQYD PFAEHRPQKI ANREDEYKQQ
RRKMIISPER LDPFADGGKT PDPKLNARTF KDVMQEQYLT KEEREIRQQI AEKAKSGDLK
VVNGSSASSA AQPPSKRKRR WDQTGDQTPG STPKKLSSWD QAEVTPGHTP SSLRWDETPG
RAKGNETPGA TPGSKIWDPT PSHTPAGVAT PGRGDTPGHV TPGHSGATSS ARKNRWDETP
KTERDTPGHG SGWAETPRTD RGGDSIGETP TPGASKRKSR WDETPASQMG GSTPVLTPGK
TPIGTPAMNM ATPTPGHIMS MTPEQLQAWR WEREIDERNR PLSDEELDAM FPEGYKVLPP
PAGYVPIRTP ARKLTATPTP LGGLTGFHMP TEDRSMKSVS DQPSGNLPFL KPDDIQYFDK
LLVDVDESTL SPEEQKERKI MKLLLKIKNG TPPMRKAALR QITDKAREFG AGPLFNQILP
LLMSPTLEDQ ERHLLVKVID RILYKLDDLV RPYVHKILVV IEPLLIDEDY YARVEGREII
SNLAKAAGLA TMISTMRPDI DNMDEYVRNT TARAFAVVAS ALGIPSLLPF LKAVCKSKKS
WQARHTGIKI VQQIAILMGC AILPHLRSLV EIIEHGLVDE QQKVRTISAL AIAALAEAAT
PYGIESFDSV LKPLWKGIRQ HRGKGLAAFL KAIGYLIPLM DAEYANYYTR EVMLILIREF
QSPDEEMKKI VLKVVKQCCG TDGVEANYIK TEILPPFFKH FWQHRMALDR RNYRQLVDTT
VELANKVGAA EIISRIVDDL KDEAEQYRKM VMETIEKIMG NLGAADIDHK LEEQLIDGIL
YAFQEQTTED SVMLNGFGTV VNALGKRVKP YLPQICGTVL WRLNNKSAKV RQQAADLISR
TAVVMKTCQE EKLMGHLGVV LYEYLGEEYP EVLGSILGAL KAIVNVIGMH KMTPPIKDLL
PRLTPILKNR HEKVQENCID LVGRIADRGA EYVSAREWMR ICFELLELLK AHKKAIRRAT
VNTFGYIAKA IGPHDVLATL LNNLKVQERQ NRVCTTVAIA IVAETCSPFT VLPALMNEYR
VPELNVQNGV LKSLSFLFEY IGEMGKDYIY AVTPLLEDAL MDRDLVHRQT ASAVVQHMSL
GVYGFGCEDS LNHLLNYVWP NVFETSPHVI QAVMGALEGL RVAIGPCRMV QYCLQGLFHP
ARKVRDVYWK IYNSIYIGSQ DALIAHYPRI YNDEKNTYIR YELDYTL
