SF3B1_YEAST
ID SF3B1_YEAST Reviewed; 971 AA.
AC P49955; D6W0B5;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=U2 snRNP component HSH155;
GN Name=HSH155; OrderedLocusNames=YMR288W; ORFNames=YM8021.14;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP IDENTIFICATION IN THE CWC COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT "Proteomics analysis reveals stable multiprotein complexes in both fission
RT and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT splicing factors, and snRNAs.";
RL Mol. Cell. Biol. 22:2011-2024(2002).
RN [4]
RP INTERACTION WITH RDS3.
RX PubMed=14517302; DOI=10.1128/mcb.23.20.7339-7349.2003;
RA Wang Q., Rymond B.C.;
RT "Rds3p is required for stable U2 snRNP recruitment to the splicing
RT apparatus.";
RL Mol. Cell. Biol. 23:7339-7349(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Contacts pre-mRNA on both sides of the branch site early in
CC spliceosome assembly. {ECO:0000250}.
CC -!- SUBUNIT: Belongs to the CWC complex (or CEF1-associated complex), a
CC spliceosome sub-complex reminiscent of a late-stage spliceosome
CC composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2,
CC CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24,
CC CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9,
CC PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3,
CC SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts
CC with RDS3. {ECO:0000269|PubMed:11884590, ECO:0000269|PubMed:14517302}.
CC -!- INTERACTION:
CC P49955; Q04693: RSE1; NbExp=3; IntAct=EBI-664, EBI-519;
CC P49955; P0C074: YSF3; NbExp=3; IntAct=EBI-664, EBI-970846;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- MISCELLANEOUS: Present with 521 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SF3B1 family. {ECO:0000305}.
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DR EMBL; Z49704; CAA89786.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10189.1; -; Genomic_DNA.
DR PIR; S54595; S54595.
DR RefSeq; NP_014015.1; NM_001182795.1.
DR PDB; 5GM6; EM; 3.50 A; G=1-971.
DR PDB; 5LQW; EM; 5.80 A; Q=1-971.
DR PDB; 5NRL; EM; 7.20 A; O=1-971.
DR PDB; 5ZWM; EM; 3.40 A; 1=1-971.
DR PDB; 5ZWO; EM; 3.90 A; 1=1-971.
DR PDB; 6G90; EM; 4.00 A; O=1-971.
DR PDB; 7OQB; EM; 9.00 A; O=1-971.
DR PDB; 7OQE; EM; 5.90 A; O=1-971.
DR PDBsum; 5GM6; -.
DR PDBsum; 5LQW; -.
DR PDBsum; 5NRL; -.
DR PDBsum; 5ZWM; -.
DR PDBsum; 5ZWO; -.
DR PDBsum; 6G90; -.
DR PDBsum; 7OQB; -.
DR PDBsum; 7OQE; -.
DR AlphaFoldDB; P49955; -.
DR SMR; P49955; -.
DR BioGRID; 35468; 77.
DR ComplexPortal; CPX-1647; SF3B complex.
DR ComplexPortal; CPX-1651; PRP19-associated complex.
DR ComplexPortal; CPX-26; U2 small nuclear ribonucleoprotein complex.
DR DIP; DIP-2628N; -.
DR IntAct; P49955; 30.
DR MINT; P49955; -.
DR STRING; 4932.YMR288W; -.
DR iPTMnet; P49955; -.
DR MaxQB; P49955; -.
DR PaxDb; P49955; -.
DR PRIDE; P49955; -.
DR EnsemblFungi; YMR288W_mRNA; YMR288W; YMR288W.
DR GeneID; 855332; -.
DR KEGG; sce:YMR288W; -.
DR SGD; S000004901; HSH155.
DR VEuPathDB; FungiDB:YMR288W; -.
DR eggNOG; KOG0213; Eukaryota.
DR GeneTree; ENSGT00390000018393; -.
DR HOGENOM; CLU_002242_0_1_1; -.
DR InParanoid; P49955; -.
DR OMA; LWHPARK; -.
DR BioCyc; YEAST:G3O-32958-MON; -.
DR PRO; PR:P49955; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P49955; protein.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0005681; C:spliceosomal complex; IC:ComplexPortal.
DR GO; GO:0005686; C:U2 snRNP; IDA:SGD.
DR GO; GO:0071004; C:U2-type prespliceosome; IDA:SGD.
DR GO; GO:0005684; C:U2-type spliceosomal complex; IPI:ComplexPortal.
DR GO; GO:0003729; F:mRNA binding; IDA:SGD.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IPI:SGD.
DR GO; GO:0000245; P:spliceosomal complex assembly; IPI:SGD.
DR GO; GO:1903241; P:U2-type prespliceosome assembly; IC:ComplexPortal.
DR Gene3D; 1.25.10.10; -; 3.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR038737; SF3b_su1-like.
DR PANTHER; PTHR12097; PTHR12097; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Repeat; Spliceosome.
FT CHAIN 1..971
FT /note="U2 snRNP component HSH155"
FT /id="PRO_0000174327"
FT REPEAT 199..237
FT /note="HEAT 1"
FT REPEAT 273..310
FT /note="HEAT 2"
FT REPEAT 350..387
FT /note="HEAT 3"
FT REPEAT 513..550
FT /note="HEAT 4"
FT REPEAT 596..633
FT /note="HEAT 5"
FT REPEAT 680..717
FT /note="HEAT 6"
FT REPEAT 722..759
FT /note="HEAT 7"
FT REPEAT 792..829
FT /note="HEAT 8"
FT REPEAT 832..870
FT /note="HEAT 9"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 54..118
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..75
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..107
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 971 AA; 110028 MW; 27D26E4252A788E2 CRC64;
MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE KMETRTVQNR
EDSYHKRRFD MKFEPDSDTQ TVTSSENTQD AVVPRKRKSR WDVKGYEPPD ESSTAVKENS
DSALVNVEGI HDLMFFKPSD HKYFADVISK KPIDELNKDE KKERTLSMLL LKIKNGNTAS
RRTSMRILTD KAVTFGPEMI FNRLLPILLD RSLEDQERHL MIKTIDRVLY QLGDLTKPYV
HKILVVAAPL LIDEDPMVRS TGQEIITNLS TVAGLKTILT VMRPDIENED EYVRNVTSRA
AAVVAKALGV NQLLPFINAA CHSRKSWKAR HTGIKIVQQI GILLGIGVLN HLTGLMSCIK
DCLMDDHVPV RIVTAHTLST LAENSYPYGI EVFNVVLEPL WKGIRSHRGK VLSSFLKAVG
SMIPLMDPEY AGYYTTEAMR IIRREFDSPD DEMKKTILLV LQKCSAVESI TPKFLREEIA
PEFFQKFWVR RVALDRPLNK VVTYTTVTLA KKLGCSYTID KLLTPLRDEA EPFRTMAVHA
VTRTVNLLGT ADLDERLETR LIDALLIAFQ EQTNSDSIIF KGFGAVTVSL DIRMKPFLAP
IVSTILNHLK HKTPLVRQHA ADLCAILIPV IKNCHEFEML NKLNIILYES LGEVYPEVLG
SIINAMYCIT SVMDLDKLQP PINQILPTLT PILRNKHRKV EVNTIKFVGL IGKLAPTYAP
PKEWMRICFE LLELLKSTNK EIRRSANATF GFIAEAIGPH DVLVALLNNL KVQERQLRVC
TAVAIGIVAK VCGPYNVLPV IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP
LLEDALTDRD LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIFE TSPHAIMRIL
EGLEALSQAL GPGLFMNYIW AGLFHPAKNV RKAFWRVYNN MYVMYQDAMV PFYPVTPDNN
EEYIEELDLV L
