ID   SF3B3_DANRE             Reviewed;        1217 AA.
AC   Q1LVE8; Q802W7;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Splicing factor 3B subunit 3;
GN   Name=sf3b3; ORFNames=zgc:55440;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in pre-mRNA splicing as a component of the splicing
CC       factor SF3B complex, a constituent of the spliceosome. SF3B complex is
CC       required for 'A' complex assembly formed by the stable binding of U2
CC       snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence
CC       independent binding of SF3A/SF3B complex upstream of the branch site is
CC       essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved
CC       in the assembly of the 'E' complex. Belongs also to the minor U12-
CC       dependent spliceosome, which is involved in the splicing of rare class
CC       of nuclear pre-mRNA intron. {ECO:0000250|UniProtKB:Q15393}.
CC   -!- SUBUNIT: Identified in the spliceosome A complex; remains associated
CC       with the spliceosome throughout the splicing process. Component of the
CC       spliceosome B complex. Identified in the spliceosome C complex.
CC       Identified in the spliceosome E complex. Component of the U11/U12
CC       snRNPs that are part of the U12-type spliceosome. Component of splicing
CC       factor SF3B complex which is composed of at least eight subunits;
CC       SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B
CC       associates with the splicing factor SF3A and a 12S RNA unit to form the
CC       U2 small nuclear ribonucleoproteins complex (U2 snRNP).
CC       {ECO:0000250|UniProtKB:Q15393}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15393}.
CC   -!- DOMAIN: The core of the protein consists of three beta-propeller
CC       domains. {ECO:0000250|UniProtKB:Q15393}.
CC   -!- SIMILARITY: Belongs to the RSE1 family. {ECO:0000305}.
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DR   EMBL; BX784024; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC047171; AAH47171.1; -; mRNA.
DR   RefSeq; NP_998668.1; NM_213503.1.
DR   AlphaFoldDB; Q1LVE8; -.
DR   SMR; Q1LVE8; -.
DR   STRING; 7955.ENSDARP00000123676; -.
DR   PaxDb; Q1LVE8; -.
DR   PRIDE; Q1LVE8; -.
DR   Ensembl; ENSDART00000159274; ENSDARP00000134109; ENSDARG00000103553.
DR   Ensembl; ENSDART00000191973; ENSDARP00000153577; ENSDARG00000103553.
DR   GeneID; 406824; -.
DR   KEGG; dre:406824; -.
DR   CTD; 23450; -.
DR   ZFIN; ZDB-GENE-040426-2901; sf3b3.
DR   eggNOG; KOG1898; Eukaryota.
DR   GeneTree; ENSGT00950000183151; -.
DR   HOGENOM; CLU_003246_0_0_1; -.
DR   InParanoid; Q1LVE8; -.
DR   OMA; FFLVQTE; -.
DR   OrthoDB; 97116at2759; -.
DR   PhylomeDB; Q1LVE8; -.
DR   TreeFam; TF105685; -.
DR   Reactome; R-DRE-72163; mRNA Splicing - Major Pathway.
DR   Reactome; R-DRE-72165; mRNA Splicing - Minor Pathway.
DR   PRO; PR:Q1LVE8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 18.
DR   Bgee; ENSDARG00000103553; Expressed in gastrula and 30 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005686; C:U2 snRNP; IBA:GO_Central.
DR   GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR   GO; GO:0030620; F:U2 snRNA binding; IBA:GO_Central.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 3.
DR   InterPro; IPR004871; Cleavage/polyA-sp_fac_asu_C.
DR   InterPro; IPR018846; Cleavage/polyA-sp_fac_asu_N.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF03178; CPSF_A; 1.
DR   Pfam; PF10433; MMS1_N; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   2: Evidence at transcript level;
KW   mRNA processing; mRNA splicing; Nucleus; Reference proteome; Spliceosome.
FT   CHAIN           1..1217
FT                   /note="Splicing factor 3B subunit 3"
FT                   /id="PRO_0000276757"
FT   CONFLICT        132
FT                   /note="I -> M (in Ref. 2; AAH47171)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        432
FT                   /note="R -> G (in Ref. 2; AAH47171)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        661
FT                   /note="G -> C (in Ref. 2; AAH47171)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1217 AA;  135622 MW;  33E96E66373E65F0 CRC64;
     MFLYNITLQR ATGISHAIHG NFSGTKQQEI VVSRGKILEL LRPDANTGKV HTLLTMEVFG
     VVRSLMAFRL TGGTKDYVVV GSDSGRIVIL EYHPSKNMFE KIHQETFGKS GCRRIVPGQF
     LAVDPKGRAV MIGATEKQKL VYILNRDAAA RLTISSPLEA HKANTLVYHV VGVDVGFENP
     MFACLEMDYE EADNDPTGEA AANTQQTLTF YELDLGLNHV VRKYSEALEE HGNFLITVPG
     GSDGPSGVLI CSENYITYKN FGDQPDIRCP IPRRRNDLDD PERGMIFVCS ATHKTKSMFF
     FLAQTEQGDI FKVTLETDEE MVTEIRMKYF DTIPVATAMC VLKTGFLFVS SEFGNHYLYQ
     IAHLGDDDEE PEFSSAMPLE EGDTFFFQPR PLKNLVLVDE QESLSPIMSC QIADLANEDT
     PQLYVACGRG PRSTLRVLRH GLEVSEMAVS ELPGNPNAVW TVRRHVEDEF DAYIIVSFVN
     ATLVLSIGET VEEVTDSGFL GTTPTLSCSL LGEDALVQVY PDGIRHIRAD KRVNEWKTPG
     KKTIIRCAVN QRQVVIALTG GELVYFEMDP SGQLNEYTER KEMSADVVCM SLANVPPGEQ
     RSRFLAVGLV DNTVRIISLD PSDCLQPLSM QALPAQPESL CIVEMGGVEK QDELGEKGTI
     GFLYLNIGLQ NGVLLRTVLD PVTGDLSDTR TRYLGSRPVK LFRVRMQGQE AVLAMSSRSW
     LSYSYQSRFH LTPLSYETLE YASGFASEQC PEGIVAISTN TLRILALEKL GAVFNQVAFP
     LQYTPRKFVI HPETNNLILI ETDHNAYTEA TKAQRKQQMA EEMVEAAGED ERELAAEMAA
     AFLNENLPEA IFGAPKAGSG QWASLVRLIN PIQGNTLDLV QLEQNEAAFS VAICRFLNGG
     DDWYVLVGVA RDMILNPRSV GGGYIYTYRI VGGGDKLEFL HKTPVEDVPL AIAPFQGRVL
     VGVGKLLRIY DLGKKKLLRK CENKHVPNLV TGIHTIGQRV IVSDVQESLF WVRYRRNENQ
     LIIFADDTYP RWITTACLLD YDTMASADKF GNICVVRLPP NTSDDVDEDP TGNKALWDRG
     LLNGASQKAE IIINYHIGET VLSLQKTTLI PGGSESLVYT TLSGGIGILV PFTSHEDHDF
     FQHLEMHMRS EFPPLCGRDH LSFRSYYFPV KNVIDGDLCE QFNSMDPHKQ KSVSEELDRT
     PPEVSKKLED IRTRYAF