SF3B3_DROME
ID SF3B3_DROME Reviewed; 1227 AA.
AC Q9W0M7; M9PDJ8; Q5BI86;
DT 20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Splicing factor 3B subunit 3 {ECO:0000312|FlyBase:FBgn0035162};
GN Name=Sf3b3 {ECO:0000312|FlyBase:FBgn0035162};
GN ORFNames=CG13900 {ECO:0000312|FlyBase:FBgn0035162};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:ADD20765.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAX33486.1, ECO:0000312|EMBL:AAX33572.1,
RC ECO:0000312|EMBL:ADD20765.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAX33486.1, ECO:0000312|EMBL:AAX33572.1};
RA Carlson J., Booth B., Chavez C., Frise E., George R., Pacleb J., Park S.,
RA Rubin G.M., Stapleton M., Wan K., Yu C., Celniker S.;
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, IDENTIFICATION IN THE SF3B
RP COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=18981222; DOI=10.1128/mcb.01415-08;
RA Herold N., Will C.L., Wolf E., Kastner B., Urlaub H., Luhrmann R.;
RT "Conservation of the protein composition and electron microscopy structure
RT of Drosophila melanogaster and human spliceosomal complexes.";
RL Mol. Cell. Biol. 29:281-301(2009).
RN [5] {ECO:0000305}
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SAGA COMPLEX,
RP AND SUBCELLULAR LOCATION.
RX PubMed=27185460; DOI=10.1016/j.jmb.2016.05.009;
RA Stegeman R., Spreacker P.J., Swanson S.K., Stephenson R., Florens L.,
RA Washburn M.P., Weake V.M.;
RT "The Spliceosomal Protein SF3B5 is a Novel Component of Drosophila SAGA
RT that Functions in Gene Expression Independent of Splicing.";
RL J. Mol. Biol. 428:3632-3649(2016).
CC -!- FUNCTION: Involved in pre-mRNA splicing as a component of the splicing
CC factor SF3B complex, a constituent of the spliceosome
CC (PubMed:18981222). SF3B complex is required for 'A' complex assembly
CC formed by the stable binding of U2 snRNP to the branchpoint sequence
CC (BPS) in pre-mRNA (PubMed:18981222). Sequence independent binding of
CC SF3A/SF3B complex upstream of the branch site is essential, it may
CC anchor U2 snRNP to the pre-mRNA (By similarity). May also be involved
CC in the assembly of the 'E' complex (By similarity). Belongs also to the
CC minor U12-dependent spliceosome, which is involved in the splicing of
CC rare class of nuclear pre-mRNA intron (By similarity).
CC {ECO:0000250|UniProtKB:Q15393, ECO:0000305|PubMed:18981222}.
CC -!- SUBUNIT: Identified in the spliceosome A complex; remains associated
CC with the spliceosome throughout the splicing process (By similarity).
CC Component of the spliceosome B complex (By similarity). Identified in
CC the spliceosome C complex (By similarity). Identified in the
CC spliceosome E complex (By similarity). Component of the U11/U12 snRNPs
CC that are part of the U12-type spliceosome (By similarity). Component of
CC splicing factor SF3B complex (PubMed:18981222). Identified in the SAGA
CC transcription regulatory histone acetylation (HAT) complex; the
CC interaction is RNA-independent (PubMed:27185460).
CC {ECO:0000250|UniProtKB:Q15393, ECO:0000269|PubMed:18981222,
CC ECO:0000269|PubMed:27185460}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18981222,
CC ECO:0000269|PubMed:27185460}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A {ECO:0000312|FlyBase:FBgn0035162};
CC IsoId=Q9W0M7-1; Sequence=Displayed;
CC Name=C {ECO:0000312|FlyBase:FBgn0035162};
CC IsoId=Q9W0M7-2; Sequence=VSP_059607, VSP_059608;
CC -!- SIMILARITY: Belongs to the RSE1 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF47416.2; -; Genomic_DNA.
DR EMBL; AE014296; AGB93918.1; -; Genomic_DNA.
DR EMBL; BT021338; AAX33486.1; -; mRNA.
DR EMBL; BT021424; AAX33572.1; -; mRNA.
DR EMBL; BT120391; ADD20765.1; -; mRNA.
DR RefSeq; NP_001261223.1; NM_001274294.2. [Q9W0M7-2]
DR RefSeq; NP_728546.1; NM_167842.3. [Q9W0M7-1]
DR AlphaFoldDB; Q9W0M7; -.
DR SMR; Q9W0M7; -.
DR IntAct; Q9W0M7; 1.
DR STRING; 7227.FBpp0072494; -.
DR PaxDb; Q9W0M7; -.
DR PRIDE; Q9W0M7; -.
DR DNASU; 38093; -.
DR EnsemblMetazoa; FBtr0072597; FBpp0072494; FBgn0035162. [Q9W0M7-1]
DR EnsemblMetazoa; FBtr0331404; FBpp0303821; FBgn0035162. [Q9W0M7-2]
DR GeneID; 38093; -.
DR KEGG; dme:Dmel_CG13900; -.
DR UCSC; CG13900-RA; d. melanogaster. [Q9W0M7-1]
DR CTD; 23450; -.
DR FlyBase; FBgn0035162; Sf3b3.
DR VEuPathDB; VectorBase:FBgn0035162; -.
DR eggNOG; KOG1898; Eukaryota.
DR GeneTree; ENSGT00950000183151; -.
DR HOGENOM; CLU_043331_0_0_1; -.
DR InParanoid; Q9W0M7; -.
DR OMA; FFLVQTE; -.
DR OrthoDB; 97116at2759; -.
DR PhylomeDB; Q9W0M7; -.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DME-72165; mRNA Splicing - Minor Pathway.
DR SignaLink; Q9W0M7; -.
DR BioGRID-ORCS; 38093; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38093; -.
DR PRO; PR:Q9W0M7; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035162; Expressed in ovary and 34 other tissues.
DR ExpressionAtlas; Q9W0M7; baseline and differential.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0000124; C:SAGA complex; IDA:UniProtKB.
DR GO; GO:0030532; C:small nuclear ribonucleoprotein complex; ISS:FlyBase.
DR GO; GO:0005686; C:U2 snRNP; IDA:UniProtKB.
DR GO; GO:0030620; F:U2 snRNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:FlyBase.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR004871; Cleavage/polyA-sp_fac_asu_C.
DR InterPro; IPR018846; Cleavage/polyA-sp_fac_asu_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF03178; CPSF_A; 1.
DR Pfam; PF10433; MMS1_N; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Spliceosome.
FT CHAIN 1..1227
FT /note="Splicing factor 3B subunit 3"
FT /id="PRO_0000444532"
FT VAR_SEQ 468..469
FT /note="DE -> GA (in isoform C)"
FT /evidence="ECO:0000305"
FT /id="VSP_059607"
FT VAR_SEQ 470..1227
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000305"
FT /id="VSP_059608"
FT CONFLICT 182
FT /note="F -> L (in Ref. 3; AAX33486)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1227 AA; 136616 MW; 3E1C4527841321FB CRC64;
MYLYNLTLQK ATGVTHAVHG NFSGGKQQEV LLSRGKSLEL LRPDSNTGKV HTLLSTEIFG
CVRALMAFRL TGGTKDYIVV GSDSGRIVIL EYNPSKNALE KVHQETFGKS GCRRIVPGQY
FAIDPKGRAV MIGAVEKQKL AYIMNRDTQA RLTISSPLEA HKSNTLTYHM VGVDVGFDNP
MFACLEIDYE EADMDPSGDA AQRTQQTLTF YELDLGLNHV VRKYSEPLEE HANFLVSVPG
GNDGPSGVLI CSENYLTYKN LGDQHDIRCP IPRRRNDLDD PERGMIFICS ATHRTKSMYF
FLLQTEQGDI FKITLETDDD VVSEIKLKYF DTVPPATAMC VLKTGFLFVA SEFGNHYLYQ
IAHLGDDDDE PEFSSAMPLE EGETFFFAPR ALKNLVLVDE LPSFAPIITS QVADLANEDT
PQLYVLCGRG PRSTLRVLRH GLEVSEMAVS ELPGNPNAVW TVKKRADDEF DAYIIVSFVN
ATLVLSIGET VEEVTDSGFL GTTPTLCCAA LGDDALVQVY PDGIRHIRSD KRVNEWKAPG
KKSITKCAVN QRQVVITLSG RELVYFEMDP TGELNEYTER SEMPAEIMCM ALGTVPEGEQ
RSWFLAVGLA DNTVRILSLD PNNCLTPCSM QALPSPAESL CLVEMGHTES TTQGGLDDDA
PAQRSGNNKG TIYLNIGLSN GVLLRTVLDP VSGDLADTRT RYLGSRPVKL FRIKMQGSEA
VLAMSSRTWL SYYHQNRFHL TPLSYETLEY ASGFSSEQCS EGIVAISTNT LRILALEKLG
AVFNQVAFPL QYTPRTFVIH PDTGRMLIAE TDHNAYTEDT KSARKEQMAE EMRSAAGDEE
RELAREMANA FINEVLPEDV FSSPKAGLGL WASQIRCLDA MHGQTMFSVP LTQNEAIMSM
AMLKFSIAAD GRYYLAVGIA KDLQLNPRIS QGGCIDIYKI DPTCSSLEFM HRTDIDEIPG
ALCGFQGRLL AGCGRMLRIY DFGKKKMLRK CENKHIPYQI VNIQAMGHRV YVSDVQESVF
FIRYRRAENQ LIIFADDTHP RWVTATTLLD YDTIAIADKF GNLSIQRLPH SVTDDVDEDP
TGTKSLWDRG LLSGASQKSE NICSFHVGEI IMSLQKATLI PGGSEALIYA TLSGTVGAFV
PFTSREDYDF FQHLEMHMRN ENPPLCGRDH LSYRSSYYPV KNVLDGDLCE QYLSIEAAKQ
KSIAGDMFRT PNQICKKLED IRTRYAF
