ID   SF3B4_CAEEL             Reviewed;         388 AA.
AC   Q09442; Q8T8N6;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 2.
DT   03-AUG-2022, entry version 144.
DE   RecName: Full=Splicing factor 3B subunit 4;
DE   AltName: Full=Spliceosome-associated protein 49;
GN   Name=sap-49; ORFNames=C08B11.5;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Subunit of the splicing factor SF3B required for 'A' complex
CC       assembly formed by the stable binding of U2 snRNP to the branchpoint
CC       sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B
CC       complex upstream of the branch site is essential, it may anchor U2
CC       snRNP to the pre-mRNA. May also be involved in the assembly of the 'E'
CC       complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs
CC       also to the minor U12-dependent spliceosome, which is involved in the
CC       splicing of rare class of nuclear pre-mRNA intron (By similarity).
CC       {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q09442; G5EC32: sorb-1; NbExp=16; IntAct=EBI-2316106, EBI-325337;
CC       Q09442; G5EE56: src-1; NbExp=3; IntAct=EBI-2316106, EBI-6538807;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SF3B4 family. {ECO:0000305}.
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DR   EMBL; Z46676; CAB60993.2; -; Genomic_DNA.
DR   PIR; T19069; T19069.
DR   RefSeq; NP_001021932.1; NM_001026761.2.
DR   AlphaFoldDB; Q09442; -.
DR   SMR; Q09442; -.
DR   BioGRID; 606934; 15.
DR   IntAct; Q09442; 15.
DR   STRING; 6239.C08B11.5; -.
DR   iPTMnet; Q09442; -.
DR   EPD; Q09442; -.
DR   PaxDb; Q09442; -.
DR   PeptideAtlas; Q09442; -.
DR   EnsemblMetazoa; C08B11.5.1; C08B11.5.1; WBGene00004723.
DR   UCSC; C08B11.5.1; c. elegans.
DR   WormBase; C08B11.5; CE36374; WBGene00004723; sap-49.
DR   eggNOG; KOG0131; Eukaryota.
DR   GeneTree; ENSGT00870000136537; -.
DR   HOGENOM; CLU_012062_21_0_1; -.
DR   InParanoid; Q09442; -.
DR   OMA; HWEQNKE; -.
DR   OrthoDB; 1479417at2759; -.
DR   PhylomeDB; Q09442; -.
DR   SignaLink; Q09442; -.
DR   PRO; PR:Q09442; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00004723; Expressed in embryo and 4 other tissues.
DR   GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR   GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR   GO; GO:0005686; C:U2 snRNP; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   CDD; cd12334; RRM1_SF3B4; 1.
DR   CDD; cd12335; RRM2_SF3B4; 1.
DR   Gene3D; 3.30.70.330; -; 2.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR034158; SF3B4_RRM1.
DR   InterPro; IPR034159; SF3B4_RRM2.
DR   Pfam; PF00076; RRM_1; 2.
DR   SMART; SM00360; RRM; 2.
DR   SUPFAM; SSF54928; SSF54928; 1.
DR   PROSITE; PS50102; RRM; 2.
PE   1: Evidence at protein level;
KW   mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat;
KW   RNA-binding; Spliceosome.
FT   CHAIN           1..388
FT                   /note="Splicing factor 3B subunit 4"
FT                   /id="PRO_0000082013"
FT   DOMAIN          13..91
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          100..179
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          244..388
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        259..388
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   388 AA;  41008 MW;  0D6B0CF04CB24C3D CRC64;
     MSAGPIVERN QDATIYVGGL DEKVSESILW ELMVQAGPVV SVNMPKDRVT ANHQGFGFVE
     FMGEEDADYA IKILNMIKLY GKPIKVNKAS AHEKNMDVGA NIFVGNLDPE VDEKLLYDTF
     SAFGVILQVP KIMRDVDSGT SKGFAFINFA SFEASDTALE AMNGQFLCNR AITVSYAFKR
     DSKGERHGTA AERMLAAQNP LFPKDRPHQV FSDVPLGVPA NTPLAMPGVH AAIAAHATGR
     PGYQPPPLMG MAQSGYQGQY PPVPPPPPSV TPMPPPMPPT PGMTPRPPPP PSSGMWPPPP
     PPPPGRTPGP PGMPGMPPPP PPSRFGPPGM GGMPPPPPPG MRYPGGMPPP PPPRYPSAGP
     GMYPPPPPSR PPAPPSGHGM IPPPPPPS