ID   SF3B4_DICDI             Reviewed;         359 AA.
AC   Q55GD6;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Splicing factor 3B subunit 4;
GN   Name=sf3b4; ORFNames=DDB_G0267714;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Subunit of the splicing factor SF3B required for 'A' complex
CC       assembly formed by the stable binding of U2 snRNP to the branchpoint
CC       sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B
CC       complex upstream of the branch site is essential, it may anchor U2
CC       snRNP to the pre-mRNA. May also be involved in the assembly of the 'E'
CC       complex. Has been found in complex 'B' and 'C' as well (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of splicing factor SF3B which is composed of at
CC       least eight subunits. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SF3B4 family. {ECO:0000305}.
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DR   EMBL; AAFI02000003; EAL73310.1; -; Genomic_DNA.
DR   RefSeq; XP_647248.1; XM_642156.1.
DR   AlphaFoldDB; Q55GD6; -.
DR   SMR; Q55GD6; -.
DR   STRING; 44689.DDB0233172; -.
DR   PaxDb; Q55GD6; -.
DR   EnsemblProtists; EAL73310; EAL73310; DDB_G0267714.
DR   GeneID; 8616053; -.
DR   KEGG; ddi:DDB_G0267714; -.
DR   dictyBase; DDB_G0267714; sf3b4.
DR   eggNOG; KOG0131; Eukaryota.
DR   HOGENOM; CLU_012062_21_0_1; -.
DR   InParanoid; Q55GD6; -.
DR   OMA; VDHTIMR; -.
DR   PhylomeDB; Q55GD6; -.
DR   PRO; PR:Q55GD6; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR   GO; GO:0005681; C:spliceosomal complex; ISS:dictyBase.
DR   GO; GO:0005686; C:U2 snRNP; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   CDD; cd12334; RRM1_SF3B4; 1.
DR   CDD; cd12335; RRM2_SF3B4; 1.
DR   Gene3D; 3.30.70.330; -; 2.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR034158; SF3B4_RRM1.
DR   InterPro; IPR034159; SF3B4_RRM2.
DR   Pfam; PF00076; RRM_1; 2.
DR   SMART; SM00360; RRM; 2.
DR   SUPFAM; SSF54928; SSF54928; 1.
DR   PROSITE; PS50102; RRM; 2.
PE   3: Inferred from homology;
KW   mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat;
KW   RNA-binding; Spliceosome.
FT   CHAIN           1..359
FT                   /note="Splicing factor 3B subunit 4"
FT                   /id="PRO_0000328584"
FT   DOMAIN          13..91
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          98..176
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          198..239
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          293..337
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        198..235
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        306..326
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   359 AA;  41184 MW;  7642075C01DB5948 CRC64;
     MASNLPQERN HEACLLIRDL DPMVTESLLM ELFIQAAPVV KVFIPKDKLT QQHSGRAYVE
     FQSSSDAEYA LKVMKFVRLF NKEIKIKKEN KDKVDIGANL FIGNLDADVD ERILHDTFSR
     FGTIIFTPKV MRDENGVSKG FAFINFDSFE ASDAAIEAMN SQFLCNKPIS VTYARKKDSN
     ERHGSSAERI IAASRNTGYL NQQQQQQSSS TSSTSTISTS TPQQQQQQQS QPLPPPIVTA
     GGIIHQQQIQ QQQQLQQQQQ QQLQLQQLQL QQLQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ
     QQHHPHHQHP IPHPHPLPHQ LRPHPHPHHP PPPPFNPMLM QFNPMMMPFG GMPPPPPHK