SF3B4_MOUSE
ID SF3B4_MOUSE Reviewed; 424 AA.
AC Q8QZY9;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Splicing factor 3B subunit 4;
GN Name=Sf3b4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Corpus striatum, and Muellerian duct;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Colon, and Jaw;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in pre-mRNA splicing as a component of the splicing
CC factor SF3B complex. SF3B complex is required for 'A' complex assembly
CC formed by the stable binding of U2 snRNP to the branchpoint sequence
CC (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex
CC upstream of the branch site is essential, it may anchor U2 snRNP to the
CC pre-mRNA. May also be involved in the assembly of the 'E' complex.
CC SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to
CC the minor U12-dependent spliceosome, which is involved in the splicing
CC of rare class of nuclear pre-mRNA intron.
CC {ECO:0000250|UniProtKB:Q15427}.
CC -!- SUBUNIT: Component of splicing factor SF3B complex which is composed of
CC at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6,
CC PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a
CC 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex
CC (U2 snRNP). Interacts directly with SF3B2. Found in a complex with
CC PRMT9, SF3B2 and SF3B4. The SF3B complex composed of SF3B1, SF3B2,
CC SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Component of
CC the U11/U12 snRNPs that are part of the U12-type spliceosome.
CC {ECO:0000250|UniProtKB:Q15427}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15427}.
CC -!- SIMILARITY: Belongs to the SF3B4 family. {ECO:0000305}.
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DR EMBL; AK047751; BAC33145.1; -; mRNA.
DR EMBL; AK135543; BAE22576.1; -; mRNA.
DR EMBL; BC024418; AAH24418.3; -; mRNA.
DR EMBL; BC026567; AAH26567.1; -; mRNA.
DR EMBL; BC085273; AAH85273.1; -; mRNA.
DR CCDS; CCDS17630.1; -.
DR RefSeq; NP_694693.1; NM_153053.4.
DR AlphaFoldDB; Q8QZY9; -.
DR SMR; Q8QZY9; -.
DR BioGRID; 223499; 5.
DR IntAct; Q8QZY9; 8.
DR STRING; 10090.ENSMUSP00000075709; -.
DR iPTMnet; Q8QZY9; -.
DR PhosphoSitePlus; Q8QZY9; -.
DR EPD; Q8QZY9; -.
DR MaxQB; Q8QZY9; -.
DR PaxDb; Q8QZY9; -.
DR PeptideAtlas; Q8QZY9; -.
DR PRIDE; Q8QZY9; -.
DR ProteomicsDB; 261177; -.
DR Antibodypedia; 20268; 336 antibodies from 37 providers.
DR DNASU; 107701; -.
DR Ensembl; ENSMUST00000076372; ENSMUSP00000075709; ENSMUSG00000068856.
DR GeneID; 107701; -.
DR KEGG; mmu:107701; -.
DR UCSC; uc008qme.1; mouse.
DR CTD; 10262; -.
DR MGI; MGI:109580; Sf3b4.
DR VEuPathDB; HostDB:ENSMUSG00000068856; -.
DR eggNOG; KOG0131; Eukaryota.
DR GeneTree; ENSGT00870000136537; -.
DR HOGENOM; CLU_012062_21_0_1; -.
DR InParanoid; Q8QZY9; -.
DR OMA; HWEQNKE; -.
DR OrthoDB; 1479417at2759; -.
DR PhylomeDB; Q8QZY9; -.
DR TreeFam; TF300890; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-MMU-72165; mRNA Splicing - Minor Pathway.
DR BioGRID-ORCS; 107701; 26 hits in 75 CRISPR screens.
DR PRO; PR:Q8QZY9; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8QZY9; protein.
DR Bgee; ENSMUSG00000068856; Expressed in embryonic post-anal tail and 92 other tissues.
DR Genevisible; Q8QZY9; MM.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005681; C:spliceosomal complex; ISO:MGI.
DR GO; GO:0005689; C:U12-type spliceosomal complex; ISO:MGI.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISO:MGI.
DR GO; GO:0005684; C:U2-type spliceosomal complex; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:1990935; F:splicing factor binding; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISO:MGI.
DR GO; GO:0048026; P:positive regulation of mRNA splicing, via spliceosome; IMP:MGI.
DR CDD; cd12334; RRM1_SF3B4; 1.
DR CDD; cd12335; RRM2_SF3B4; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR034158; SF3B4_RRM1.
DR InterPro; IPR034159; SF3B4_RRM2.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Acetylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; RNA-binding; Spliceosome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q15427"
FT CHAIN 2..424
FT /note="Splicing factor 3B subunit 4"
FT /id="PRO_0000328585"
FT DOMAIN 13..91
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 100..179
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 207..424
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 232..274
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 327..424
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q15427"
FT MOD_RES 56
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q15427"
SQ SEQUENCE 424 AA; 44356 MW; 7B6B69A2BD95F1E4 CRC64;
MAAGPISERN QDATVYVGGL DEKVSEPLLW ELFLQAGPVV NTHMPKDRVT GQHQGYGFVE
FLSEEDADYA IKIMNMIKLY GKPIRVNKAS AHNKNLDVGA NIFIGNLDPE IDEKLLYDTF
SAFGVILQTP KIMRDPDTGN SKGYAFINFA SFDASDAAIE AMNGQYLCNR PITVSYAFKK
DSKGERHGSA AERLLAAQNP LSQADRPHQL FADAPPPPSA PNPVVSSLGS GLPPPGMPPP
GSFPPPVPPP GALPPGIPPA MPPPPMPPGA GGHGPPAAGT PGAGHPGHGH SHPHPFPPGG
MPHPGMSQMQ LAHHGPHGLG HPHAGPPGSG GQPPPRPPPG MPHPGPPPMG MPPRGPPFGS
PMGHPGPMPP HGMRGPPPLM PPHGYTGPPR PPPYGYQRGP LPPPRPTPRP PVPPRGPLRG
PLPQ
