SF3B5_DROME
ID SF3B5_DROME Reviewed; 85 AA.
AC Q9VHI4;
DT 11-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Splicing factor 3B subunit 5 {ECO:0000312|FlyBase:FBgn0040534};
DE AltName: Full=Pre-mRNA-splicing factor SF3b 10 kDa subunit;
GN Name=Sf3b5 {ECO:0000312|FlyBase:FBgn0040534};
GN ORFNames=CG11985 {ECO:0000312|FlyBase:FBgn0040534};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, IDENTIFICATION IN THE SF3B
RP COMPLEX, SUBCELLULAR LOCATION, AND ELECTRON MICROSCOPY OF THE SF3B COMPLEX.
RX PubMed=18981222; DOI=10.1128/mcb.01415-08;
RA Herold N., Will C.L., Wolf E., Kastner B., Urlaub H., Luhrmann R.;
RT "Conservation of the protein composition and electron microscopy structure
RT of Drosophila melanogaster and human spliceosomal complexes.";
RL Mol. Cell. Biol. 29:281-301(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, IDENTIFICATION IN THE SAGA
RP COMPLEX, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=27185460; DOI=10.1016/j.jmb.2016.05.009;
RA Stegeman R., Spreacker P.J., Swanson S.K., Stephenson R., Florens L.,
RA Washburn M.P., Weake V.M.;
RT "The Spliceosomal Protein SF3B5 is a Novel Component of Drosophila SAGA
RT that Functions in Gene Expression Independent of Splicing.";
RL J. Mol. Biol. 428:3632-3649(2016).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27035939; DOI=10.1073/pnas.1513682113;
RA Wu H., Sun L., Wen Y., Liu Y., Yu J., Mao F., Wang Y., Tong C., Guo X.,
RA Hu Z., Sha J., Liu M., Xia L.;
RT "Major spliceosome defects cause male infertility and are associated with
RT nonobstructive azoospermia in humans.";
RL Proc. Natl. Acad. Sci. U.S.A. 113:4134-4139(2016).
CC -!- FUNCTION: Involved in pre-mRNA splicing as component of spliceosome
CC (PubMed:27185460, PubMed:18981222). As part of the spliceosome complex,
CC plays a role in the regulation of spermatogonial differentiation
CC (PubMed:27035939). When associated with the SAGA transcription
CC regulatory histone acetylation (HAT) complex, might be involved in the
CC transcriptional activation of a subset of SAGA-regulated genes
CC (PubMed:27185460). {ECO:0000269|PubMed:27035939,
CC ECO:0000269|PubMed:27185460, ECO:0000305|PubMed:18981222}.
CC -!- SUBUNIT: Component of the SF3B complex (PubMed:27185460,
CC PubMed:18981222). SF3B complex associates with the splicing factor SF3A
CC complex and a 12S RNA unit to form the U2 small nuclear
CC ribonucleoproteins complex (U2 snRNP)(PubMed:27185460,
CC PubMed:18981222). Identified in the SAGA transcription regulatory
CC histone acetylation (HAT) complex; the interaction is RNA-independent
CC (PubMed:27185460). {ECO:0000269|PubMed:18981222,
CC ECO:0000269|PubMed:27185460}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18981222,
CC ECO:0000269|PubMed:27185460}.
CC -!- DISRUPTION PHENOTYPE: Lethal at the first instar larval stage
CC (PubMed:27185460). RNAi-mediated knockdown in germ cells results in
CC male sterility (PubMed:27035939). {ECO:0000269|PubMed:27035939,
CC ECO:0000269|PubMed:27185460}.
CC -!- SIMILARITY: Belongs to the SF3B5 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014297; AAF54327.1; -; Genomic_DNA.
DR RefSeq; NP_652189.1; NM_143932.3.
DR AlphaFoldDB; Q9VHI4; -.
DR SMR; Q9VHI4; -.
DR BioGRID; 72500; 45.
DR DIP; DIP-20303N; -.
DR IntAct; Q9VHI4; 1.
DR STRING; 7227.FBpp0081484; -.
DR PaxDb; Q9VHI4; -.
DR DNASU; 50007; -.
DR EnsemblMetazoa; FBtr0082006; FBpp0081484; FBgn0040534.
DR GeneID; 50007; -.
DR KEGG; dme:Dmel_CG11985; -.
DR UCSC; CG11985-RA; d. melanogaster.
DR CTD; 83443; -.
DR FlyBase; FBgn0040534; Sf3b5.
DR VEuPathDB; VectorBase:FBgn0040534; -.
DR eggNOG; KOG3485; Eukaryota.
DR GeneTree; ENSGT00390000013215; -.
DR HOGENOM; CLU_138804_3_1_1; -.
DR InParanoid; Q9VHI4; -.
DR OMA; PMLSYMA; -.
DR OrthoDB; 1632322at2759; -.
DR PhylomeDB; Q9VHI4; -.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DME-72165; mRNA Splicing - Minor Pathway.
DR BioGRID-ORCS; 50007; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 50007; -.
DR PRO; PR:Q9VHI4; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0040534; Expressed in imaginal disc and 16 other tissues.
DR ExpressionAtlas; Q9VHI4; baseline and differential.
DR Genevisible; Q9VHI4; DM.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0000124; C:SAGA complex; IDA:UniProtKB.
DR GO; GO:0005686; C:U2 snRNP; IDA:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR InterPro; IPR009846; SF3b5/RDS3-10.
DR InterPro; IPR017089; Splicing_factor_3B_subunit_5.
DR PANTHER; PTHR20978; PTHR20978; 1.
DR Pfam; PF07189; SF3b10; 1.
DR PIRSF; PIRSF037010; Splicing_factor_3B_subunit_5; 1.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Spliceosome.
FT CHAIN 1..85
FT /note="Splicing factor 3B subunit 5"
FT /id="PRO_0000220759"
SQ SEQUENCE 85 AA; 9971 MW; 91128BC196462BF2 CRC64;
MGERYNIHSQ LEHLQSKYIG TGHADTTKFE WLTNQHRDSL ASYMGHYDIL NYFAIAENES
KARVRFNLME RMLQPCGPPP EKLED
