SF3B5_MOUSE
ID SF3B5_MOUSE Reviewed; 86 AA.
AC Q923D4;
DT 11-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Splicing factor 3B subunit 5;
DE Short=SF3b5;
DE AltName: Full=Pre-mRNA-splicing factor SF3b 10 kDa subunit;
GN Name=Sf3b5; Synonyms=Sf3b10;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in pre-mRNA splicing as a component of the splicing
CC factor SF3B complex, a constituent of the spliceosome. SF3B complex is
CC required for 'A' complex assembly formed by the stable binding of U2
CC snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence
CC independent binding of SF3A/SF3B complex upstream of the branch site is
CC essential, it may anchor U2 snRNP to the pre-mRNA.
CC {ECO:0000250|UniProtKB:Q9BWJ5}.
CC -!- SUBUNIT: Component of the spliceosome B complex. Component of splicing
CC factor SF3B complex which is composed of at least eight subunits;
CC SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B
CC associates with the splicing factor SF3A and a 12S RNA unit to form the
CC U2 small nuclear ribonucleoproteins complex (U2 snRNP). Within the SF3B
CC complex interacts directly with SF3B1 (via HEAT domain) and SF3B3. The
CC SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and
CC PHF5A interacts with U2AF2. Component of the U11/U12 snRNPs that are
CC part of the U12-type spliceosome. {ECO:0000250|UniProtKB:Q9BWJ5}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9BWJ5}.
CC -!- SIMILARITY: Belongs to the SF3B5 family. {ECO:0000305}.
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DR EMBL; AK003475; BAC25037.1; -; mRNA.
DR EMBL; AK009874; BAC25275.1; -; mRNA.
DR EMBL; BC006603; AAH06603.1; -; mRNA.
DR CCDS; CCDS35843.1; -.
DR RefSeq; NP_780311.2; NM_175102.4.
DR AlphaFoldDB; Q923D4; -.
DR SMR; Q923D4; -.
DR BioGRID; 211233; 11.
DR ComplexPortal; CPX-6803; SAGA complex, KAT2B variant.
DR ComplexPortal; CPX-920; SAGA complex, KAT2A variant.
DR IntAct; Q923D4; 3.
DR STRING; 10090.ENSMUSP00000100771; -.
DR iPTMnet; Q923D4; -.
DR PhosphoSitePlus; Q923D4; -.
DR SwissPalm; Q923D4; -.
DR CPTAC; non-CPTAC-3876; -.
DR EPD; Q923D4; -.
DR MaxQB; Q923D4; -.
DR PaxDb; Q923D4; -.
DR PeptideAtlas; Q923D4; -.
DR PRIDE; Q923D4; -.
DR ProteomicsDB; 261507; -.
DR Antibodypedia; 46681; 44 antibodies from 18 providers.
DR DNASU; 66125; -.
DR Ensembl; ENSMUST00000105139; ENSMUSP00000100771; ENSMUSG00000078348.
DR GeneID; 66125; -.
DR KEGG; mmu:66125; -.
DR UCSC; uc007eki.2; mouse.
DR CTD; 83443; -.
DR MGI; MGI:1913375; Sf3b5.
DR VEuPathDB; HostDB:ENSMUSG00000078348; -.
DR eggNOG; KOG3485; Eukaryota.
DR GeneTree; ENSGT00390000013215; -.
DR HOGENOM; CLU_138804_3_1_1; -.
DR InParanoid; Q923D4; -.
DR OMA; PMLSYMA; -.
DR OrthoDB; 1632322at2759; -.
DR PhylomeDB; Q923D4; -.
DR TreeFam; TF300117; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-MMU-72165; mRNA Splicing - Minor Pathway.
DR BioGRID-ORCS; 66125; 23 hits in 70 CRISPR screens.
DR ChiTaRS; Sf3b5; mouse.
DR PRO; PR:Q923D4; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q923D4; protein.
DR Bgee; ENSMUSG00000078348; Expressed in internal carotid artery and 257 other tissues.
DR Genevisible; Q923D4; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR GO; GO:0000124; C:SAGA complex; IC:ComplexPortal.
DR GO; GO:0005689; C:U12-type spliceosomal complex; ISO:MGI.
DR GO; GO:0005686; C:U2 snRNP; IBA:GO_Central.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0005684; C:U2-type spliceosomal complex; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; ISO:MGI.
DR GO; GO:1990935; F:splicing factor binding; ISO:MGI.
DR GO; GO:0043966; P:histone H3 acetylation; IC:ComplexPortal.
DR GO; GO:0035521; P:monoubiquitinated histone deubiquitination; ISO:MGI.
DR GO; GO:0035522; P:monoubiquitinated histone H2A deubiquitination; ISO:MGI.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IC:ComplexPortal.
DR GO; GO:0006282; P:regulation of DNA repair; IC:ComplexPortal.
DR GO; GO:0043484; P:regulation of RNA splicing; IC:ComplexPortal.
DR InterPro; IPR009846; SF3b5/RDS3-10.
DR InterPro; IPR017089; Splicing_factor_3B_subunit_5.
DR PANTHER; PTHR20978; PTHR20978; 1.
DR Pfam; PF07189; SF3b10; 1.
DR PIRSF; PIRSF037010; Splicing_factor_3B_subunit_5; 1.
PE 1: Evidence at protein level;
KW Acetylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; RNA-binding; Spliceosome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9BWJ5"
FT CHAIN 2..86
FT /note="Splicing factor 3B subunit 5"
FT /id="PRO_0000220758"
FT REGION 15..76
FT /note="Interaction with SF3B1 and SF3B3"
FT /evidence="ECO:0000250|UniProtKB:Q9BWJ5"
FT SITE 5
FT /note="Interaction with RNA"
FT /evidence="ECO:0000250|UniProtKB:Q9BWJ5"
FT SITE 20
FT /note="Interaction with RNA"
FT /evidence="ECO:0000250|UniProtKB:Q9BWJ5"
FT MOD_RES 2
FT /note="N-acetylthreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9BWJ5"
FT MOD_RES 9
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BWJ5"
FT MOD_RES 17
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9BWJ5"
SQ SEQUENCE 86 AA; 10119 MW; B8A21DBA9F548E8E CRC64;
MTDRYTIHSQ LEHLQSKYIG TGHADTTKWE WLVNQHRDSY CSYMGHFDLL NYFAIAENES
KARVRFNLME KMLQPSGPPA DKPEEN
