SF3B6_DROME
ID SF3B6_DROME Reviewed; 121 AA.
AC Q9VRV7;
DT 11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Splicing factor 3B subunit 6 {ECO:0000312|FlyBase:FBgn0035692};
DE AltName: Full=Pre-mRNA branch site p14-like protein;
GN Name=Sf3b6 {ECO:0000312|FlyBase:FBgn0035692};
GN ORFNames=CG13298 {ECO:0000312|FlyBase:FBgn0035692};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAL90341.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SF3B COMPLEX,
RP AND ELECTRON MICROSCOPY OF THE SF3B COMPLEX.
RX PubMed=18981222; DOI=10.1128/mcb.01415-08;
RA Herold N., Will C.L., Wolf E., Kastner B., Urlaub H., Luhrmann R.;
RT "Conservation of the protein composition and electron microscopy structure
RT of Drosophila melanogaster and human spliceosomal complexes.";
RL Mol. Cell. Biol. 29:281-301(2009).
CC -!- FUNCTION: Involved in pre-mRNA splicing as a component of the splicing
CC factor SF3B complex (Probable). SF3B complex is required for 'A'
CC complex assembly formed by the stable binding of U2 snRNP to the
CC branchpoint sequence (BPS) in pre-mRNA (By similarity). Directly
CC contacts the pre-mRNA branch site adenosine for the first catalytic
CC step of splicing (By similarity). Enters the spliceosome and associates
CC with the pre-mRNA branch site as part of the 17S U2 or, in the case of
CC the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and
CC thus may facilitate the interaction of these snRNP with the branch
CC sites of U2 and U12 respectively (By similarity).
CC {ECO:0000250|UniProtKB:Q9Y3B4, ECO:0000305|PubMed:18981222}.
CC -!- SUBUNIT: Component of splicing factor SF3B complex (PubMed:18981222).
CC Component of the U11/U12 snRNPs that are part of the U12-type
CC spliceosome (By similarity). {ECO:0000250|UniProtKB:Q9Y3B4,
CC ECO:0000269|PubMed:18981222}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18981222}.
CC -!- SIMILARITY: Belongs to the SF3B6 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF50675.1; -; Genomic_DNA.
DR EMBL; AY089603; AAL90341.1; -; mRNA.
DR RefSeq; NP_648037.1; NM_139780.3.
DR AlphaFoldDB; Q9VRV7; -.
DR SMR; Q9VRV7; -.
DR BioGRID; 64173; 6.
DR DIP; DIP-21174N; -.
DR IntAct; Q9VRV7; 1.
DR STRING; 7227.FBpp0076723; -.
DR PaxDb; Q9VRV7; -.
DR PRIDE; Q9VRV7; -.
DR DNASU; 38720; -.
DR EnsemblMetazoa; FBtr0077015; FBpp0076723; FBgn0035692.
DR GeneID; 38720; -.
DR KEGG; dme:Dmel_CG13298; -.
DR UCSC; CG13298-RA; d. melanogaster.
DR CTD; 51639; -.
DR FlyBase; FBgn0035692; Sf3b6.
DR VEuPathDB; VectorBase:FBgn0035692; -.
DR eggNOG; KOG0114; Eukaryota.
DR GeneTree; ENSGT00390000005908; -.
DR HOGENOM; CLU_012062_25_2_1; -.
DR InParanoid; Q9VRV7; -.
DR OMA; GYNFMDR; -.
DR OrthoDB; 1603846at2759; -.
DR PhylomeDB; Q9VRV7; -.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DME-72165; mRNA Splicing - Minor Pathway.
DR BioGRID-ORCS; 38720; 1 hit in 1 CRISPR screen.
DR ChiTaRS; Sf3b6; fly.
DR GenomeRNAi; 38720; -.
DR PRO; PR:Q9VRV7; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035692; Expressed in wing disc and 64 other tissues.
DR ExpressionAtlas; Q9VRV7; baseline and differential.
DR Genevisible; Q9VRV7; DM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0005689; C:U12-type spliceosomal complex; IBA:GO_Central.
DR GO; GO:0005686; C:U2 snRNP; IBA:GO_Central.
DR GO; GO:0005684; C:U2-type spliceosomal complex; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; ISS:FlyBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd12241; RRM_SF3B14; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR034150; SF3B6_RRM.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..121
FT /note="Splicing factor 3B subunit 6"
FT /id="PRO_0000081728"
FT DOMAIN 15..90
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 12..25
FT /note="Interaction with pre-mRNA branch site"
FT /evidence="ECO:0000250"
SQ SEQUENCE 121 AA; 14194 MW; 926D5B5199CF6652 CRC64;
MNKRNHIRLP PEVNRLLYVR NLPYKITSDE MYDIFGKFGA IRQIRVGNTP ETRGTAFVVY
EDIFDAKNAC DHLSGFNVCN RYLVVLYYQS NKAFKRVDMD KKQEELNNIK AKYNLKTPEA
P
