ID   SFAA_STAA8              Reviewed;         397 AA.
AC   Q2FW71;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Staphyloferrin A transporter {ECO:0000305};
GN   Name=sfaA {ECO:0000303|PubMed:25680529};
GN   OrderedLocusNames=SAOUHSC_02435 {ECO:0000312|EMBL:ABD31457.1};
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=25680529; DOI=10.1016/j.febslet.2015.02.002;
RA   Hannauer M., Sheldon J.R., Heinrichs D.E.;
RT   "Involvement of major facilitator superfamily proteins SfaA and SbnD in
RT   staphyloferrin secretion in Staphylococcus aureus.";
RL   FEBS Lett. 589:730-737(2015).
CC   -!- FUNCTION: Involved in staphyloferrin A secretion.
CC       {ECO:0000269|PubMed:25680529}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the gene impacts growth in iron-
CC       depleted media. Mutant accumulates staphyloferrin A in the cytoplasm.
CC       Does not affect staphyloferrin B levels. {ECO:0000269|PubMed:25680529}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP000253; ABD31457.1; -; Genomic_DNA.
DR   RefSeq; WP_000170403.1; NZ_LS483365.1.
DR   RefSeq; YP_500904.1; NC_007795.1.
DR   AlphaFoldDB; Q2FW71; -.
DR   STRING; 1280.SAXN108_2428; -.
DR   EnsemblBacteria; ABD31457; ABD31457; SAOUHSC_02435.
DR   GeneID; 3919000; -.
DR   KEGG; sao:SAOUHSC_02435; -.
DR   PATRIC; fig|93061.5.peg.2196; -.
DR   eggNOG; COG2814; Bacteria.
DR   HOGENOM; CLU_034180_11_2_9; -.
DR   OMA; VQVWHVY; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR010290; TM_effector.
DR   Pfam; PF05977; MFS_3; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..397
FT                   /note="Staphyloferrin A transporter"
FT                   /id="PRO_0000447124"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        67..87
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        162..182
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        213..233
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        245..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        271..292
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        296..313
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        333..353
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        358..378
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   397 AA;  44337 MW;  08DD69DE5FD6FAD5 CRC64;
     MTKYFFSSSF LLFLGNWIGQ IGLNWFVLTT YHNAVYLGIV NFCRLVPILL LSVWAGAIAD
     KYDKGRLLRI TISSSFLVTA ILCVLTYSFT AIPISVIIIY ATLRGILSAV ETPLRQAILP
     DLSDKISTTQ AVSFHSFIIN ICRSIGPAIA GVILAVYHAP TTFLAQAICY FIAVLLCLPL
     HFKVTKIPED ASRYMPLKVI IDYFKLHMEG RQIFITSLLI MATGFSYTTL LPVLTNKVFP
     GKSEIFGIAM TMCAIGGIIA TLVLPKVLKY IGMVNMYYLS SFLFGIALLG VVFHNIVIMF
     ICITLIGLFS QWARTTNRVY FQNNVKDYER GKVLSIIMMD RGMIPLGSLL MSICADVFGI
     VRTFSIMGIS TICITMVFYF INRKLKLKLE ESNHGIS