ID   SFAS_DUNBI              Reviewed;         277 AA.
AC   P54214;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=SF-assemblin;
OS   Dunaliella bioculata (Green alga).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Dunaliellaceae; Dunaliella.
OX   NCBI_TaxID=13790;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=SAG 19-4;
RX   PubMed=8718674; DOI=10.1242/jcs.109.4.827;
RA   Lechtreck K.-F., Frins S., Bilski J., Teltenkoetter A., Weber K.,
RA   Melkonian M.;
RT   "The cruciated microtubule-associated fibers of the green alga Dunaliella
RT   bioculata consist of a 31 kDa SF-assemblin.";
RL   J. Cell Sci. 109:827-835(1996).
CC   -!- FUNCTION: Major component of the striated microtubule-associated fibers
CC       (SMAFs; system-I-fibers). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}.
CC   -!- DOMAIN: Consists of a small non-helical N-terminal domain and a rod
CC       domain with a 29 residue repeat pattern based on four heptads followed
CC       by a skip residue. This alpha-helical protein is characterized by the
CC       ability to form a special segmented coiled coil and to assemble into
CC       striated fibers of 2 nm protofilaments.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the SF-assemblin family. {ECO:0000305}.
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DR   EMBL; X90905; CAA62413.1; -; mRNA.
DR   AlphaFoldDB; P54214; -.
DR   SMR; P54214; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0005200; F:structural constituent of cytoskeleton; IEA:InterPro.
DR   InterPro; IPR008374; SF_assemblin/giardin_b.
DR   PANTHER; PTHR40412; PTHR40412; 1.
DR   Pfam; PF06705; SF-assemblin; 1.
DR   PRINTS; PR01799; SFASSEMBLIN.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Direct protein sequencing;
KW   Microtubule.
FT   CHAIN           1..277
FT                   /note="SF-assemblin"
FT                   /id="PRO_0000221442"
FT   REGION          1..27
FT                   /note="Nonhelical region"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          28..277
FT                   /note="Rod"
FT   COILED          70..90
FT                   /evidence="ECO:0000255"
FT   CONFLICT        75
FT                   /note="M -> Q (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        106
FT                   /note="C -> E (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   277 AA;  31412 MW;  618AAC306A7094B7 CRC64;
     MATSGMVSPT SGRPFSPMRS SVLTTTGSAI KLEHVSEKFA GLWTDLEQEK QARRIAEATR
     MQLFQESVLR LEKSMEAEVK RRAESDKQLQ AHFEGELRVL HERSACQVAE MHNSLKSAID
     SFSSRVQDLH AVIREERDQR RNDIEHLATS LVGKVNECVA ALDEERTIRM QEQAMALKRF
     GEDISGVNHR VDTEKAQREA DLSGLRVEIH DVLANRNIQD EQFKTVTLDE LNAFKAALAL
     EREERVAEDD EIVQAINDYT RALQEGLKMV NMQHNSA