ID   SFAS_SPESI              Reviewed;         284 AA.
AC   P55925;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=SF-assemblin;
OS   Spermatozopsis similis (Green alga).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Dunaliellaceae; Spermatozopsis.
OX   NCBI_TaxID=3192;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=8491776; DOI=10.1083/jcb.121.4.837;
RA   Weber K., Geisler N., Plessmann U., Bremerich A., Lechtreck K.-F.,
RA   Melkonian M.;
RT   "SF-assemblin, the structural protein of the 2-nm filaments from striated
RT   microtubule associated fibers of algal flagellar roots, forms a segmented
RT   coiled coil.";
RL   J. Cell Biol. 121:837-845(1993).
CC   -!- FUNCTION: Major component of the striated microtubule-associated fibers
CC       (SMAFs; system-I-fibers).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC   -!- DOMAIN: Consists of a small non-helical N-terminal domain and a rod
CC       domain with a 29 residue repeat pattern based on four heptads followed
CC       by a skip residue. This alpha-helical protein is characterized by the
CC       ability to form a special segmented coiled coil and to assemble into
CC       striated fibers of 2 nm protofilaments.
CC   -!- PTM: Consists of at least four isoforms including two phosphorylated.
CC   -!- SIMILARITY: Belongs to the SF-assemblin family. {ECO:0000305}.
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DR   PIR; A40689; A40689.
DR   AlphaFoldDB; P55925; -.
DR   SMR; P55925; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0005200; F:structural constituent of cytoskeleton; IEA:InterPro.
DR   InterPro; IPR008374; SF_assemblin/giardin_b.
DR   PANTHER; PTHR40412; PTHR40412; 1.
DR   Pfam; PF06705; SF-assemblin; 1.
DR   PRINTS; PR01799; SFASSEMBLIN.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Direct protein sequencing;
KW   Microtubule; Phosphoprotein.
FT   CHAIN           1..284
FT                   /note="SF-assemblin"
FT                   /id="PRO_0000221443"
FT   REGION          1..38
FT                   /note="Nonhelical region"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          39..284
FT                   /note="Rod"
FT   COILED          56..102
FT                   /evidence="ECO:0000255"
FT   COILED          239..268
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   284 AA;  32076 MW;  0BB27A43477B8FCF CRC64;
     PTPSPEARVA SRPFLDSPLP GSPRSGSPTG YITATKAISA GKLEHVAEKF SNFYNEIELE
     KQQRRMADAA RFQMLTDSIA KLEKSLEAEI KRRAESDKQI QVHFESEVKG LQERTALQLA
     DLQAAFKTSV DGLSRTMQDL HTIIKEEREQ RRSDIEHLAG SLVNKVNECV AALDEERISR
     MDAESKILKQ IATDVFRVQE KIDTEKGTRE AELATLRSEI HEVLGNRNLS DEKFQTLVLD
     EINNLKSAVQ MEREERISED DEIVQAVNDY TRALQDGLRI VNNS