SFC1_YEAST
ID SFC1_YEAST Reviewed; 322 AA.
AC P33303; D6VWR4;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=Succinate/fumarate mitochondrial transporter;
DE AltName: Full=Regulator of acetyl-CoA synthase activity;
GN Name=SFC1; Synonyms=ACR1; OrderedLocusNames=YJR095W; ORFNames=J1921;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7908717; DOI=10.1007/bf00283428;
RA Fernandez M., Fernandez E., Rodicio R.;
RT "ACR1, a gene encoding a protein related to mitochondrial carriers, is
RT essential for acetyl-CoA synthetase activity in Saccharomyces cerevisiae.";
RL Mol. Gen. Genet. 242:727-735(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP CHARACTERIZATION.
RX PubMed=9395087; DOI=10.1016/s0014-5793(97)01269-6;
RA Palmieri L., Lasorsa F.M., De Palma A., Palmieri F., Runswick M.J.,
RA Walker J.E.;
RT "Identification of the yeast ACR1 gene product as a succinate-fumarate
RT transporter essential for growth on ethanol or acetate.";
RL FEBS Lett. 417:114-118(1997).
CC -!- FUNCTION: Transports cytoplasmic succinate, derived from isocitrate by
CC the action of isocitrate lyase in the cytosol, into the mitochondrial
CC matrix in exchange for fumarate.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC protein.
CC -!- INDUCTION: By ethanol or acetate as sole carbon sources. Repressed by
CC glucose.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; Z25485; CAA80973.1; -; Genomic_DNA.
DR EMBL; Z49595; CAA89624.1; -; Genomic_DNA.
DR EMBL; AY692924; AAT92943.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08880.1; -; Genomic_DNA.
DR PIR; S57116; S57116.
DR RefSeq; NP_012629.1; NM_001181753.1.
DR AlphaFoldDB; P33303; -.
DR BioGRID; 33850; 77.
DR DIP; DIP-8019N; -.
DR IntAct; P33303; 2.
DR MINT; P33303; -.
DR STRING; 4932.YJR095W; -.
DR TCDB; 2.A.29.13.1; the mitochondrial carrier (mc) family.
DR MaxQB; P33303; -.
DR PaxDb; P33303; -.
DR PRIDE; P33303; -.
DR TopDownProteomics; P33303; -.
DR EnsemblFungi; YJR095W_mRNA; YJR095W; YJR095W.
DR GeneID; 853558; -.
DR KEGG; sce:YJR095W; -.
DR SGD; S000003856; SFC1.
DR VEuPathDB; FungiDB:YJR095W; -.
DR eggNOG; KOG0756; Eukaryota.
DR HOGENOM; CLU_015166_5_0_1; -.
DR InParanoid; P33303; -.
DR OMA; YEWYKQL; -.
DR BioCyc; YEAST:G3O-31723-MON; -.
DR PRO; PR:P33303; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P33303; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005469; F:succinate:fumarate antiporter activity; IDA:SGD.
DR GO; GO:0015741; P:fumarate transport; IDA:SGD.
DR GO; GO:0015744; P:succinate transport; IDA:SGD.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..322
FT /note="Succinate/fumarate mitochondrial transporter"
FT /id="PRO_0000090679"
FT TRANSMEM 11..31
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..193
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..235
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..295
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 8..99
FT /note="Solcar 1"
FT REPEAT 111..202
FT /note="Solcar 2"
FT REPEAT 212..303
FT /note="Solcar 3"
FT CONFLICT 300..322
FT /note="VREHLENLGIFKKNDTPKPKPLK -> RKGAFQKIWVYSRRMTHQSQSH
FT (in Ref. 1; CAA80973)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 322 AA; 35340 MW; EC29718A0F5011A7 CRC64;
MSQKKKASHP AINLMAGGTA GLFEALCCHP LDTIKVRMQI YRRVAGIEHV KPPGFIKTGR
TIYQKEGFLA LYKGLGAVVI GIIPKMAIRF SSYEFYRTLL VNKESGIVST GNTFVAGVGA
GITEAVLVVN PMEVVKIRLQ AQHLTPSEPN AGPKYNNAIH AAYTIVKEEG VSALYRGVSL
TAARQATNQG ANFTVYSKLK EFLQNYHQMD VLPSWETSCI GLISGAIGPF SNAPLDTIKT
RLQKDKSISL EKQSGMKKII TIGAQLLKEE GFRALYKGIT PRVMRVAPGQ AVTFTVYEYV
REHLENLGIF KKNDTPKPKP LK
