SFGH1_ECOUT
ID SFGH1_ECOUT Reviewed; 277 AA.
AC Q1RFI8;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=S-formylglutathione hydrolase FrmB;
DE Short=FGH;
DE EC=3.1.2.12;
GN Name=frmB; OrderedLocusNames=UTI89_C0375;
OS Escherichia coli (strain UTI89 / UPEC).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=364106;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTI89 / UPEC;
RX PubMed=16585510; DOI=10.1073/pnas.0600938103;
RA Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A.,
RA Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S.,
RA Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J.,
RA Gordon J.I.;
RT "Identification of genes subject to positive selection in uropathogenic
RT strains of Escherichia coli: a comparative genomics approach.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006).
CC -!- FUNCTION: Serine hydrolase involved in the detoxification of
CC formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + S-formylglutathione = formate + glutathione + H(+);
CC Xref=Rhea:RHEA:14961, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:57688, ChEBI:CHEBI:57925; EC=3.1.2.12;
CC -!- SIMILARITY: Belongs to the esterase D family. {ECO:0000305}.
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DR EMBL; CP000243; ABE05876.1; -; Genomic_DNA.
DR RefSeq; WP_000419070.1; NC_007946.1.
DR AlphaFoldDB; Q1RFI8; -.
DR SMR; Q1RFI8; -.
DR ESTHER; ecoli-yaim; A85-EsteraseD-FGH.
DR MEROPS; S09.940; -.
DR EnsemblBacteria; ABE05876; ABE05876; UTI89_C0375.
DR KEGG; eci:UTI89_C0375; -.
DR HOGENOM; CLU_056472_0_0_6; -.
DR OMA; YFWRRMA; -.
DR Proteomes; UP000001952; Chromosome.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0018738; F:S-formylglutathione hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0046294; P:formaldehyde catabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000801; Esterase-like.
DR InterPro; IPR014186; S-formylglutathione_hydrol.
DR PANTHER; PTHR10061; PTHR10061; 1.
DR Pfam; PF00756; Esterase; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR TIGRFAMs; TIGR02821; fghA_ester_D; 1.
PE 3: Inferred from homology;
KW Hydrolase; Serine esterase.
FT CHAIN 1..277
FT /note="S-formylglutathione hydrolase FrmB"
FT /id="PRO_0000341657"
FT ACT_SITE 145
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 221
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 254
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
SQ SEQUENCE 277 AA; 31435 MW; A16D0455C3693BA0 CRC64;
MELIEKHASF GGWQNVYRHY SQSLKCEMNV GVYLPPKAEN EKLPVLYWLS GLTCNEQNFI
TKSGMQRYAA EHNIIVVAPD TSPRGSHVAD ADRYDLGQGA GFYLNATQAP WNEHYKMYDY
IRNELPNLVM HHFPATARKS ISGHSMGGLG ALVLALRNPD EYVSVSAFSP IVSPSQVPWG
QQAFAAYLGE NKDAWLDYDP VSLISQGQRV AEIMVDQGLS DDFYAEQLRT PNLEKICQEM
NIKTLIRYHE GYDHSYYFVS SFIGEHIAYH ANKLNMR