SFGH1_SHISS
ID SFGH1_SHISS Reviewed; 277 AA.
AC Q3Z551;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=S-formylglutathione hydrolase FrmB;
DE Short=FGH;
DE EC=3.1.2.12;
GN Name=frmB; OrderedLocusNames=SSON_0334;
OS Shigella sonnei (strain Ss046).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=300269;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ss046;
RX PubMed=16275786; DOI=10.1093/nar/gki954;
RA Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J.,
RA Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J.,
RA Xu J., Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J.,
RA Jin Q.;
RT "Genome dynamics and diversity of Shigella species, the etiologic agents of
RT bacillary dysentery.";
RL Nucleic Acids Res. 33:6445-6458(2005).
CC -!- FUNCTION: Serine hydrolase involved in the detoxification of
CC formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + S-formylglutathione = formate + glutathione + H(+);
CC Xref=Rhea:RHEA:14961, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:57688, ChEBI:CHEBI:57925; EC=3.1.2.12;
CC -!- SIMILARITY: Belongs to the esterase D family. {ECO:0000305}.
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DR EMBL; CP000038; AAZ87111.1; -; Genomic_DNA.
DR AlphaFoldDB; Q3Z551; -.
DR SMR; Q3Z551; -.
DR ESTHER; shiss-yaim; A85-EsteraseD-FGH.
DR EnsemblBacteria; AAZ87111; AAZ87111; SSON_0334.
DR KEGG; ssn:SSON_0334; -.
DR HOGENOM; CLU_056472_0_0_6; -.
DR OMA; YFWRRMA; -.
DR Proteomes; UP000002529; Chromosome.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0018738; F:S-formylglutathione hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0046294; P:formaldehyde catabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000801; Esterase-like.
DR InterPro; IPR014186; S-formylglutathione_hydrol.
DR PANTHER; PTHR10061; PTHR10061; 1.
DR Pfam; PF00756; Esterase; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR TIGRFAMs; TIGR02821; fghA_ester_D; 1.
PE 3: Inferred from homology;
KW Hydrolase; Serine esterase.
FT CHAIN 1..277
FT /note="S-formylglutathione hydrolase FrmB"
FT /id="PRO_0000341664"
FT ACT_SITE 145
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 221
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 254
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
SQ SEQUENCE 277 AA; 31399 MW; D87B291FAFA5AA9A CRC64;
MELIEKHASF GGWQNVYRHY SQSLKCEMNV GVYLPPKAAN EKLPVLYWLS GLTCNEQNFI
TKSGMQRYAA EHNIIVVAPD TSPRGSHVAD ADRYDLGQGA GFYLNATQAP WNEHYKMYDY
IRNELPDLVM QHFPATTRKS ISGHSMGGLG ALVLALRNPD EYVSVSAFSP IVSPSQVPWG
QQAFAAYLGE NKDAWLDYDP VSLISQGQRV AEIMVDQGLS DDFYAEQLRT PNLEKICQEM
NIKTLIRYHE GYDHSYYFVS SFIGEHIAYH ANKLNMR