ID   SFGH_HAEIN              Reviewed;         275 AA.
AC   P44556;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=S-formylglutathione hydrolase;
DE            Short=FGH;
DE            EC=3.1.2.12;
GN   OrderedLocusNames=HI_0184;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=10675023;
RX   DOI=10.1002/(sici)1522-2683(20000101)21:2<411::aid-elps411>3.0.co;2-4;
RA   Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C.,
RA   Fountoulakis M.;
RT   "Two-dimensional map of the proteome of Haemophilus influenzae.";
RL   Electrophoresis 21:411-429(2000).
CC   -!- FUNCTION: Serine hydrolase involved in the detoxification of
CC       formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + S-formylglutathione = formate + glutathione + H(+);
CC         Xref=Rhea:RHEA:14961, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:57688, ChEBI:CHEBI:57925; EC=3.1.2.12;
CC   -!- SIMILARITY: Belongs to the esterase D family. {ECO:0000305}.
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DR   EMBL; L42023; AAC21853.1; -; Genomic_DNA.
DR   PIR; A64145; A64145.
DR   RefSeq; NP_438352.1; NC_000907.1.
DR   RefSeq; WP_005694102.1; NC_000907.1.
DR   AlphaFoldDB; P44556; -.
DR   SMR; P44556; -.
DR   STRING; 71421.HI_0184; -.
DR   ESTHER; haein-sfgh; A85-EsteraseD-FGH.
DR   EnsemblBacteria; AAC21853; AAC21853; HI_0184.
DR   KEGG; hin:HI_0184; -.
DR   PATRIC; fig|71421.8.peg.188; -.
DR   eggNOG; COG0627; Bacteria.
DR   HOGENOM; CLU_056472_0_0_6; -.
DR   OMA; TFMEDHL; -.
DR   PhylomeDB; P44556; -.
DR   BioCyc; HINF71421:G1GJ1-194-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0018738; F:S-formylglutathione hydrolase activity; IBA:GO_Central.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:InterPro.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000801; Esterase-like.
DR   InterPro; IPR014186; S-formylglutathione_hydrol.
DR   PANTHER; PTHR10061; PTHR10061; 1.
DR   Pfam; PF00756; Esterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   TIGRFAMs; TIGR02821; fghA_ester_D; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome; Serine esterase.
FT   CHAIN           1..275
FT                   /note="S-formylglutathione hydrolase"
FT                   /id="PRO_0000210344"
FT   ACT_SITE        145
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        221
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        254
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   275 AA;  31317 MW;  781F5C0411546D3D CRC64;
     MKLIEQHQIF GGSQQVWAHN AQTLQCEMKF AVYLPNNPEN RPLGVIYWLS GLTCTEQNFI
     TKSGFQRYAA EHQVIVVAPD TSPRGEQVPN DAAYDLGQGA GFYLNATEQP WATNYQMYDY
     ILNELPDLIE ANFPTNGKRS IMGHSMGGHG ALVLALRNRE RYQSVSAFSP ILSPSLVPWG
     EKAFSAYLGE DREKWQQYDA SSLIQQGYKV QGMRIDQGLE DEFLPTQLRT EDFIETCRVA
     NQPVDVRFHK GYDHSYYFIA SFIGEHIAYH AEFLK