BGLB_ACET2
ID BGLB_ACET2 Reviewed; 755 AA.
AC P14002; A3DEV9;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Thermostable beta-glucosidase B;
DE EC=3.2.1.21;
DE AltName: Full=Beta-D-glucoside glucohydrolase;
DE AltName: Full=Cellobiase;
DE AltName: Full=Gentiobiase;
GN Name=bglB; OrderedLocusNames=Cthe_1256;
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX PubMed=2505054; DOI=10.1007/bf00330944;
RA Graebnitz F., Ruecknagel K.P., Seiss M., Staudenbauer W.L.;
RT "Nucleotide sequence of the Clostridium thermocellum bgIB gene encoding
RT thermostable beta-glucosidase B: homology to fungal beta-glucosidases.";
RL Mol. Gen. Genet. 217:70-76(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC with release of beta-D-glucose.; EC=3.2.1.21;
CC -!- PATHWAY: Glycan metabolism; cellulose degradation.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 3 family. {ECO:0000305}.
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DR EMBL; X15644; CAA33665.1; -; Genomic_DNA.
DR EMBL; CP000568; ABN52488.1; -; Genomic_DNA.
DR PIR; S04381; S04381.
DR RefSeq; WP_003517480.1; NC_009012.1.
DR AlphaFoldDB; P14002; -.
DR SMR; P14002; -.
DR STRING; 203119.Cthe_1256; -.
DR CAZy; GH3; Glycoside Hydrolase Family 3.
DR EnsemblBacteria; ABN52488; ABN52488; Cthe_1256.
DR KEGG; cth:Cthe_1256; -.
DR eggNOG; COG1472; Bacteria.
DR HOGENOM; CLU_004542_4_1_9; -.
DR OMA; VEPAYEF; -.
DR OrthoDB; 949956at2; -.
DR UniPathway; UPA00696; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0008422; F:beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 3.20.20.300; -; 1.
DR Gene3D; 3.40.50.1700; -; 1.
DR InterPro; IPR026891; Fn3-like.
DR InterPro; IPR019800; Glyco_hydro_3_AS.
DR InterPro; IPR002772; Glyco_hydro_3_C.
DR InterPro; IPR036881; Glyco_hydro_3_C_sf.
DR InterPro; IPR001764; Glyco_hydro_3_N.
DR InterPro; IPR036962; Glyco_hydro_3_N_sf.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR Pfam; PF14310; Fn3-like; 1.
DR Pfam; PF00933; Glyco_hydro_3; 1.
DR Pfam; PF01915; Glyco_hydro_3_C; 1.
DR PRINTS; PR00133; GLHYDRLASE3.
DR SMART; SM01217; Fn3_like; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF52279; SSF52279; 1.
DR PROSITE; PS00775; GLYCOSYL_HYDROL_F3; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Cellulose degradation; Direct protein sequencing;
KW Glycosidase; Hydrolase; Polysaccharide degradation; Reference proteome.
FT CHAIN 1..755
FT /note="Thermostable beta-glucosidase B"
FT /id="PRO_0000210778"
FT ACT_SITE 231
FT /evidence="ECO:0000250"
FT CONFLICT 125
FT /note="S -> P (in Ref. 1; CAA33665)"
FT /evidence="ECO:0000305"
FT CONFLICT 287
FT /note="F -> I (in Ref. 1; CAA33665)"
FT /evidence="ECO:0000305"
FT CONFLICT 300..301
FT /note="DK -> EQ (in Ref. 1; CAA33665)"
FT /evidence="ECO:0000305"
FT CONFLICT 371
FT /note="G -> A (in Ref. 1; CAA33665)"
FT /evidence="ECO:0000305"
FT CONFLICT 481..493
FT /note="ALADVLFGEVNPS -> RWRMCYSVKSIV (in Ref. 1; CAA33665)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 755 AA; 83900 MW; 944A4D782E6AEAB8 CRC64;
MAVDIKKIIK QMTLEEKAGL CSGLDFWHTK PVERLGIPSI MMTDGPHGLR KQREDAEIAD
INNSVPATCF PSAAGLACSW DRELVERVGA ALGEECQAEN VSILLGPGAN IKRSPLCGRN
FEYFSEDPYL SSELAASHIK GVQSQGVGAC LKHFAANNQE HRRMTVDTIV DERTLREIYF
ASFENAVKKA RPWVVMCAYN KLNGEYCSEN RYLLTEVLKN EWMHDGFVVS DWGAVNDRVS
GLDAGLDLEM PTSHGITDKK IVEAVKSGKL SENILNRAVE RILKVIFMAL ENKKENAQYD
KDAHHRLARQ AAAESMVLLK NEDDVLPLKK SGTIALIGAF VKKPRYQGSG SSHITPTRLD
DIYEEIKKAG GDKVNLVYSE GYRLENDGID EELINEAKKA ASSSDVAVVF AGLPDEYESE
GFDRTHMSIP ENQNRLIEAV AEVQSNIVVV LLNGSPVEMP WIDKVKSVLE AYLGGQALGG
ALADVLFGEV NPSGKLAETF PVKLSHNPSY LNFPGEDDRV EYKEGLFVGY RYYDTKGIEP
LFPFGHGLSY TKFEYSDISV DKKDVSDNSI INVSVKVKNV GKMAGKEIVQ LYVKDVKSSV
RRPEKELKGF EKVFLNPGEE KTVTFTLDKR AFAYYNTQIK DWHVESGEFL ILIGRSSRDI
VLKESVRVNS TVKIRKRFTV NSAVEDVMSD SSAAAVLGPV LKEITDALQI DMDNAHDMMA
ANIKNMPLRS LVGYSQGRLS EEMLEELVDK INNVE
