ID   SFH8_ARATH              Reviewed;         633 AA.
AC   F4IHJ0; Q67YM1; Q9SJS7;
DT   18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Phosphatidylinositol/phosphatidylcholine transfer protein SFH8;
DE   AltName: Full=Protein SEC FOURTEEN HOMOLOGS 8;
DE            Short=AtSFH8;
GN   Name=SFH8; OrderedLocusNames=At2g21520; ORFNames=F3K23.28;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 62-633.
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY.
RX   PubMed=15728190; DOI=10.1083/jcb.200412074;
RA   Vincent P., Chua M., Nogue F., Fairbrother A., Mekeel H., Xu Y., Allen N.,
RA   Bibikova T.N., Gilroy S., Bankaitis V.A.;
RT   "A Sec14p-nodulin domain phosphatidylinositol transfer protein polarizes
RT   membrane growth of Arabidopsis thaliana root hairs.";
RL   J. Cell Biol. 168:801-812(2005).
RN   [5]
RP   REVIEW.
RX   PubMed=17051233; DOI=10.1038/nchembio835;
RA   Ile K.E., Schaaf G., Bankaitis V.A.;
RT   "Phosphatidylinositol transfer proteins and cellular nanoreactors for lipid
RT   signaling.";
RL   Nat. Chem. Biol. 2:576-583(2006).
CC   -!- FUNCTION: Required for transport of secretory proteins from the Golgi
CC       complex. Catalyzes the transfer of phosphatidylinositol and
CC       phosphatidylcholine between membranes in vitro (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}. Cell membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=F4IHJ0-1; Sequence=Displayed;
CC   -!- SIMILARITY: Belongs to the SFH family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD23696.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC006841; AAD23696.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC07189.1; -; Genomic_DNA.
DR   EMBL; AK176447; BAD44210.1; -; mRNA.
DR   PIR; C84602; C84602.
DR   RefSeq; NP_179747.4; NM_127724.7. [F4IHJ0-1]
DR   AlphaFoldDB; F4IHJ0; -.
DR   SMR; F4IHJ0; -.
DR   STRING; 3702.AT2G21520.2; -.
DR   PaxDb; F4IHJ0; -.
DR   PRIDE; F4IHJ0; -.
DR   EnsemblPlants; AT2G21520.1; AT2G21520.1; AT2G21520. [F4IHJ0-1]
DR   GeneID; 816691; -.
DR   Gramene; AT2G21520.1; AT2G21520.1; AT2G21520. [F4IHJ0-1]
DR   KEGG; ath:AT2G21520; -.
DR   Araport; AT2G21520; -.
DR   eggNOG; KOG1471; Eukaryota.
DR   HOGENOM; CLU_014001_11_1_1; -.
DR   OMA; ASPKEEF; -.
DR   PRO; PR:F4IHJ0; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; F4IHJ0; baseline and differential.
DR   Genevisible; F4IHJ0; AT.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   CDD; cd00170; SEC14; 1.
DR   Gene3D; 3.40.525.10; -; 1.
DR   InterPro; IPR001251; CRAL-TRIO_dom.
DR   InterPro; IPR036865; CRAL-TRIO_dom_sf.
DR   InterPro; IPR011074; CRAL/TRIO_N_dom.
DR   InterPro; IPR036273; CRAL/TRIO_N_dom_sf.
DR   Pfam; PF00650; CRAL_TRIO; 1.
DR   Pfam; PF03765; CRAL_TRIO_N; 1.
DR   SMART; SM01100; CRAL_TRIO_N; 1.
DR   SMART; SM00516; SEC14; 1.
DR   SUPFAM; SSF46938; SSF46938; 1.
DR   SUPFAM; SSF52087; SSF52087; 1.
DR   PROSITE; PS50191; CRAL_TRIO; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell membrane; Coiled coil; Golgi apparatus;
KW   Membrane; Protein transport; Reference proteome; Transport.
FT   CHAIN           1..633
FT                   /note="Phosphatidylinositol/phosphatidylcholine transfer
FT                   protein SFH8"
FT                   /id="PRO_0000423468"
FT   DOMAIN          152..326
FT                   /note="CRAL-TRIO"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00056"
FT   REGION          19..70
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          503..534
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          571..593
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        19..42
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        49..66
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        503..518
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   633 AA;  71725 MW;  952657466C42E065 CRC64;
     MSGPLDRFAR PCFEGFLSSD EKKERKSDFE NSEDERRTRI GSLKKKAINA STKFKHSLKK
     KSGRRKSDGR VSSVSIEDVR DVEELQAVDA FRQSLLMDEL LPDRHDDYHM MLRFLKARKF
     DVEKAKQMWA DMIQWRKEFG TDTIIQDFDF EEINEVLKHY PQCYHGVDKE GRPIYIERLG
     KVDPNRLMQV TSMDRYVRYH VKEFERSFMI KFPSCTISAK RHIDSSTTIL DVQGVGLKNF
     NKSARDLITR LQKIDGDNYP ETLHQMFIIN AGPGFRLLWN TVKSFLDPKT SAKIHVLGYK
     YLSKLLEVID VNELPEFLGG ACTCADQGGC MLSDKGPWKN PEIVKMVLHG GAHRARQVVK
     VLNSEGKVIA YAKPSYTWIK GSDTSTAESG SDAEDIGSPK AIKSFSHLRL TPVREEAKIA
     GETSLAGSFP GYDEYVPMVD KAVDATWKVK PAIQRVASRG ALMSPTVPKD HEGIKARVLV
     MFMAFLMAVF TFFRTVTKKL PATTTSSPAE TQGNAIELGS NGEGVKEECR PPSPVPDLTE
     TDLLNCVTKK LTELEGKIGT LQSKPNEMPY EKEELLNAAV CRVDALEAEL IATKKALYEA
     LMRQEELLAY IDRQEEAQFQ KMKKKKKKHL FCF